Tag: 100-200

Huang, He;Yuan, Min;Seitzer, Phillip;Ludwigsen, Susan;Asara, John M. published 《IsoSearch: an untargeted and unbiased metaboliteand lipid isotopomer tracing strategy fromHR-LC-MS/MS datasets》. The research results were published in《Methods and Protocols》 in 2020.Recommanded Product: 6-Aminonicotinamide The article conveys some information:

Stable isotopic tracer anal. is a technique used to determine carbon or nitrogen atom incorporation into biol. systems. A number of mass spectrometry based approaches have been developed for this purpose, including high-resolution tandem mass spectrometry (HR-LC-MS/MS), selected reaction monitoring (SRM) and parallel reaction monitoring (PRM). We have developed an approach for analyzing untargeted metabolomic and lipidomic datasets using high-resolution mass spectrometry with polarity switching and implemented our approach in the open-source R script IsoSearch and in Scaffold Elements software. Using our strategy, which requires an unlabeled reference dataset and isotope labeled datasets across various biol. conditions, we traced metabolic isotopomer alterations in breast cancer cells (MCF-7) treated with the metabolic drugs 2-deoxy-glucose, 6-aminonicotinamide, compound 968, and rapamycin. Metabolites and lipids were first identified by the com. software Scaffold Elements and LipidSearch, then IsoSearch successfully profiled the 13C-isotopomers extracted metabolites and lipids from 13C-glucose labeled MCF-7 cells. The results interpreted known models, such as glycolysis and pentose phosphate pathway inhibition, but also helped to discover new metabolic/lipid flux patterns, including a reactive oxygen species (ROS) defense mechanism induced by 6AN and triglyceride accumulation in rapamycin treated cells. The results suggest the IsoSearch/Scaffold Elements platform is effective for studying metabolic tracer anal. in diseases, drug metabolism, and metabolic engineering for both polar metabolites and non-polar lipids. The experimental procedure involved many compounds, such as 6-Aminonicotinamide (cas: 329-89-5) .

6-Aminonicotinamide (cas:329-89-5)Recommanded Product: 6-Aminonicotinamide induces apoptosis in tumor cells. It is clinically used in disseminated neoplastic disease. It also acts as 6-phosphogluconate dehydrogenase inhibitor. It aids in the treatment of psoriasis. It is used as cancer chemotherapeutic drug in animals.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Miao, Yongchao;Wen, Shuming;Feng, Qicheng;Liao, Runpeng published 《Enhanced adsorption of salicylhydroxamic acid on ilmenite surfaces modified by Fenton and its effect on floatability》 in 2021. The article was appeared in 《Colloids and Surfaces, A: Physicochemical and Engineering Aspects》. They have made some progress in their research.Name: N,2-Dihydroxybenzamide The article mentions the following:

This work reports a new method of using Fenton to oxidize and modify the ilmenite surface using salicylhydroxamic acid (SHA) as the collector. The activation mechanism of this process was studied by analyzing changes that occurred on the ilmenite surface. Micro-flotation showed that the maximum recovery of ilmenite after treatment with Fenton increased by 37% and attributed to the increase in the adsorbed SHA amount on the ilmenite surface. Zeta potential and XPS measurement data showed that Fenton promoted the conversion of Fe2+→Fe3+ on the ilmenite surface that enhanced SHA adsorption. After adding Fenton, the Fe-SHA complex (formed in the pulp) interacted with the O sites of ilmenite surfaces, and improved the ilmenite floatability. The IR spectra of the ilmenite+Fenton+SHA system showed several intense bands that indicated enhanced SHA chemisorption on the ilmenite surface. Thus, ilmenite treatment with the Fenton promoted its surface reactivity caused SHA to bind with the ilmenite surface and enhanced the ilmenite floatability.N,2-Dihydroxybenzamide (cas: 89-73-6) were involved in the experimental procedure.

N,2-Dihydroxybenzamide(cas: 89-73-6) is widely used for a variety of roles in biology and medicine as a chelating therapy.Name: N,2-DihydroxybenzamideIt inhibits bacterial or fungi growth by interfering with iron uptake. It is also active as a inhibitor of enzyme involved in tumour growths.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Liu, Ying;Zhang, Hua;Cui, Yan;Zheng, Yanli;Chen, Hui;Hu, Zhangli;Wu, Qingyu published 《Distinct roles of alternative oxidase pathway during the greening process of etiolated algae》 in 2021. The article was appeared in 《Science China: Life Sciences》. They have made some progress in their research.Safety of N,2-Dihydroxybenzamide The article mentions the following:

The vital function of mitochondrial alternative oxidase (AOX) pathway in optimizing photosynthesis during plant de-etiolation has been well recognized. However, whether and how AOX impacts the chloroplast biogenesis in algal cells remains unclear. In the present study, the role of AOX in regulating the reassembly of chloroplast in algal cells was investigated by treating Auxenochlorella protothecoides with salicylhydroxamic acid (SHAM), the specific inhibitor to AOX, in the heterotrophy to autotrophy transition process. Several lines of evidences including delayed chlorophyll accumulation, lagged reorganization of chloroplast structure, altered PSI/PSII stoichiometry, and declined photosynthetic activities in SHAM treated cells indicated that the impairment in AOX activity dramatically hindered the development of functioning chloroplast in algal cells. Besides, the cellular ROS levels and antioxidant enzymes activities were increased by SHAM treatment, and the perturbation on the balance of NAD⁺/NADH and NADP⁺/NADPH ratios was also observed in A. protothecoides lacking AOX activity, indicating that AOX was essential in promoting ROS scavenging and keeping the redox homeostasis for algal chloroplast development during greening. Our study revealed the essentiality of mitochondrial AOX pathway in sustaining algal photosynthetic performance and provided novel insights into the physiol. roles of AOX on the biogenesis of photosynthetic organelle in algae.N,2-Dihydroxybenzamide (cas: 89-73-6) were involved in the experimental procedure.

N,2-Dihydroxybenzamide(cas: 89-73-6) has also been shown to be active against wild-type strains of Candida glabrata, but not against resistant mutants. Safety of N,2-Dihydroxybenzamide This drug may have therapeutic potential for bone cancer and metabolic disorders such as obesity.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Recommanded Product: N,2-DihydroxybenzamideIn 2020, Salerno, Elvin V.;Eliseeva, Svetlana V.;Schneider, Bernadette L.;Kampf, Jeff W.;Petoud, Stephane;Pecoraro, Vincent L. published 《Visible, Near-Infrared, and Dual-Range Luminescence Spanning the 4f Series Sensitized by a Gallium(III)/Lanthanide(III) Metallacrown Structure》. 《Journal of Physical Chemistry A》published the findings. The article contains the following contents:

Ga³⁺/Ln³⁺ metallacrowns (MCs) [LnGa₈(shi)₈(OH)₄]Na·xMeOH·yH₂O (Ln-1, Ln = Pr³⁺, Nd³⁺, Sm³⁺-Yb³⁺ and analog Y³⁺; H₃shi = salicylhydroxamic acid) is presented. Ln-1 were obtained by reacting Ga³⁺ and Ln³⁺ nitrate salts with the H₃shi ligand. X-ray single crystal unit cell anal. confirmed that all MCs are isostructural. The crystal structure was solved for the Nd³⁺ analog and revealed that Nd³⁺ is centered between 2 [12-MC_Ga^III_N(shi)-4] MC rings and bound to 8 hydroximate O ions (4 from each ring) in a pseudosquare antiprismatic fashion adopting a pseudo-D_4h symmetry. Pulsed gradient spin echo diffusion ordered ¹H NMR spectroscopy and electrospray ionization mass spectrometry confirmed that the structure of Ln-1 remains intact in MeOH solutions while mass spectrometry suggests that 4 OH^– bridges are exchanged with MeO^–/CD₃O^–. An exceptional ability of this series of MCs to sensitize the characteristic emission of Ln³⁺ was confirmed with the observation of bright red and green emission signals of Eu-1 and Tb-1, NIR emissions of Yb-1 and Nd-1, and dual-range emissions of Pr-1, Sm-1, Dy-1, Ho-1, Er-1, and Tm-1 in the solid state upon excitation into ligand-centered bands at 340 nm. The luminescence properties of Ln-1 (Ln = Nd³⁺, Sm³⁺, Eu³⁺, Tb³⁺, Dy³⁺, and Yb³⁺) were also studied in MeOH and CD₃OD solutions For Eu-1 and Yb-1 MCs, more extensive analyses of the photophys. properties were performed, which included the determination of radiative lifetimes, intrinsic quantum yields, and sensitization efficiencies. The absolute quantum yields (Q_Ln^L) of Ln-1 in the visible and NIR ranges were determined In the case of Sm-1, the values of Q_Ln^L in MeOH and CD₃OD solutions are exceptionally high, i.e., 10.1(5) and 83(1)%. Values obtained for Yb-1, i.e., 0.78(4)% in MeOH and 8.4(1)% in CD₃OD, are among the highest ones reported today for Yb³⁺ complexes formed with nondeuterated and nonhalogenated ligands. The experimental procedure involved many compounds, such as N,2-Dihydroxybenzamide (cas: 89-73-6) .

N,2-Dihydroxybenzamide(cas: 89-73-6) can be used:To prepare phenylboronic acid-based bioconjugates for chromatographic applications;As a ligand to synthesize Fe(III), Cu(II), Ni(II) and Zn(II) complexes.Recommanded Product: N,2-Dihydroxybenzamide

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Nagahori, Hirohisa;Suzuki, Noriyuki;Le Coz, Florian;Omori, Takashi;Saito, Koichi published 《Prediction of in vivo developmental toxicity by combination of Hand1-Luc embryonic stem cell test and metabolic stability test with clarification of metabolically inapplicable candidates》. The research results were published in《Toxicology Letters》 in 2016.Recommanded Product: 329-89-5 The article conveys some information:

Hand1-Luc Embryonic Stem Cell Test (Hand1-Luc EST) is a promising alternative method for evaluation of developmental toxicity. However, the problems of predictivity have remained due to appropriateness of the solubility, metabolic system, and prediction model. Therefore, we assessed the usefulness of rat liver S9 metabolic stability test using LC-MS/MS to develop new prediction model. A total of 71 chems. were analyzed by measuring cytotoxicity and differentiation toxicity, and highly reproducible (CV = 20%) results were obtained. The first prediction model was developed by discriminant anal. performed on a full dataset using Hand1-Luc EST, and 66.2% of the chems. were correctly classified by the cross-validated classification. A second model was developed with addnl. descriptors obtained from the metabolic stability test to calculate hepatic availability, and an accuracy of 83.3% was obtained with applicability domain of 50.7% (=36/71) after exclusion of 22 metabolically inapplicable candidates, which potentially have a metabolic activation property. A step-wise prediction scheme with combination of Hand1-Luc EST and metabolic stability test was therefore proposed. The current results provide a promising in vitro test method for accurately predicting in vivo developmental toxicity. And 6-Aminonicotinamide (cas: 329-89-5) was used in the research process.

6-Aminonicotinamide (cas:329-89-5)Recommanded Product: 329-89-5 is an aminopyridine, which is a specific pentose inhibitor and thus inhibits the NADP production.It can be used as a reactant for the synthesis of 6-substituted imidazo[1,2-a]pyridines with potential application as chemotherapeutic drugs.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Application of 89-73-6In 2021, Chen, Tomer;Cohen, Dekel;Itkin, Maxim;Malitsky, Sergey;Fluhr, Robert published 《Lipoxygenase functions in ¹O₂ production during root responses to osmotic stress》. 《Plant Physiology》published the findings. The article contains the following contents:

Drought induces osmotic stress in roots, a condition simulated by the application of high-mol.-weight polyethylene glycol. Osmotic stress results in the reduction of Arabidopsis thaliana root growth and production of 1O2 from an unknown non-photosynthetic source. Reduced root growth can be alleviated by application of the 1O2 scavenger histidine (HIS). Here, we examined the possibility that 1O2 production involves Russell reactions occurring among the enzymic products of lipoxygenases (LOXs), the fatty acid hydroperoxides. LOX activity was measured for purified soybean (Glycine max) LOX1 and in crude Arabidopsis root extracts using linoleic acid as substrate. Formation of the 13(S)-Hydroperoxy-9(Z),11(E)-octadecadienoic acid product was inhibited by salicylhdroxamic acid, which is a LOX inhibitor, but not by HIS, whereas 1O2 production was inhibited by both. D2O, which specifically extends the half-life of 1O2, augmented the LOX-dependent generation of 1O2, as expected from a Russell-type reaction. The addition of linoleic acid to roots stimulated 1O2 production and inhibited growth, suggesting that the availability of LOX substrate is a rate-limiting step. Indeed, water stress rapidly increased linoleic and linolenic acids by 2.5-fold in roots. Mutants with root-specific microRNA repression of LOXs showed downregulation of LOX protein and activity. The lines with downregulated LOX displayed significantly less 1O2 formation, improved root growth in osmotic stress, and an altered transcriptome response compared with wild type. The results show that LOXs can serve as an enzymic source of dark 1O2 during osmotic stress and demonstrate a role for 1O2 in defining the physiol. response. To complete the study, the researchers used N,2-Dihydroxybenzamide (cas: 89-73-6) .

N,2-Dihydroxybenzamide(cas: 89-73-6) can be used:To prepare phenylboronic acid-based bioconjugates for chromatographic applications;As a ligand to synthesize Fe(III), Cu(II), Ni(II) and Zn(II) complexes.Application of 89-73-6

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Zhou, Yanhong;Xia, Xiaojian;Yu, Gaobo;Wang, Jitao;Wu, Jingxue;Wang, Mengmeng;Yang, Youxin;Shi, Kai;Yu, Yunlong;Chen, Zhixiang;Gan, Jay;Yu, Jingquan published 《Brassinosteroids play a critical role in the regulation of pesticide metabolism in crop plants》 in 2015. The article was appeared in 《Scientific Reports》. They have made some progress in their research.Recommanded Product: 329-89-5 The article mentions the following:

Pesticide residues in agricultural produce pose a threat to human health worldwide. Although the detoxification mechanisms for xenobiotics have been extensively studied in mammalian cells, information about the regulation network in plants remains elusive. Here we show that brassinosteroids (BRs), a class of natural plant hormones, decreased residues of common organophosphorus, organochlorine and carbamate pesticides by 30-70% on tomato, rice, tea, broccoli, cucumber, strawberry, and other plants when treated externally. Genome-wide microarray anal. showed that fungicide chlorothalonil (CHT) and BR co-upregulated 301 genes, including a set of detoxifying genes encoding cytochrome P 450, oxidoreductase, hydrolase and transferase in tomato plants. The level of BRs was closely related to the respiratory burst oxidase 1 (RBOH1)-encoded NADPH oxides-dependent H₂O₂ production, glutathione biosynthesis and the redox homeostasis, and the activity of glutathione S-transferase (GST). Gene silencing treatments showed that BRs decreased pesticide residues in plants likely by promoting their metabolism through a signaling pathway involving BRs-induced H₂O₂ production and cellular redox change. Our study provided a novel approach for minimizing pesticide residues in crops by exploiting plants’ own detoxification mechanisms. The experimental procedure involved many compounds, such as 6-Aminonicotinamide (cas: 329-89-5) .

6-Aminonicotinamide (cas:329-89-5)Recommanded Product: 329-89-5 induces apoptosis in tumor cells. It is clinically used in disseminated neoplastic disease. It also acts as 6-phosphogluconate dehydrogenase inhibitor. It aids in the treatment of psoriasis. It is used as cancer chemotherapeutic drug in animals.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Mazorra Morales, Luis Miguel;Cosme Silva, Glaucia Michelle;Santana, Diederson Bortolini;Pireda, Saulo F.;Dorighetto Cogo, Antonio Jesus;Heringer, Angelo Schuabb;de Oliveira, Tadeu dos Reis;Reis, Ricardo S.;dos Santos Prado, Luis Alfredo;de Oliveira, Andre Vicente;Silveira, Vanildo;Da Cunha, Maura;Barros, Claudia F.;Facanha, Arnoldo R.;Baldet, Pierre;Bartoli, Carlos G.;da Silva, Marcelo Gomes;Oliveira, Jurandi G. published 《Mitochondrial dysfunction associated with ascorbate synthesis in plants》 in 2022. The article was appeared in 《Plant Physiology and Biochemistry (Issy-les-Moulineaux, France)》. They have made some progress in their research.Product Details of 89-73-6 The article mentions the following:

Mitochondria are the major organelles of energy production; however, active mitochondria can decline their energetic role and show a dysfunctional status. Mitochondrial dysfunction was induced by high non-physiol. level of L-galactone-1,4-lactone (L-GalL), the precursor of ascorbate (AsA), in plant mitochondria. The dysfunction induced by L-GalL was associated with the fault in the mitochondrial electron partition and reactive oxygen species (ROS) over-production Using mitochondria from RNAi-plant lines harbouring silenced L-galactone-1,4-lactone dehydrogenase (L-GalLDH) activity, it was demonstrated that such dysfunction is dependent on this enzyme activity. The capacity of alternative respiration was strongly decreased by L-GalL, probably mediated by redox-inactivation of the alternative oxidase (AOX) enzyme. Although, alternative respiration was shown to be the key factor that helps support AsA synthesis in dysfunctional mitochondria. Experiments with respiratory inhibitors showed that ROS formation and mitochondrial dysfunction were more associated with the decline in the activities of COX (cytochrome oxidase) and particularly AOX than with the lower activities of respiratory complexes I and III. The application of high L-GalL concentrations induced proteomic changes that indicated alterations in proteins related to oxidative stress and energetic status. However, supra-optimal L-GalL concentration was not deleterious for plants. Instead, the L-GalLDH activity could be pos. Indeed, it was found that wild type plants performed better growth than L-GalLDH-RNAi plants in response to high non-physiol. L-GalL concentrations The experimental procedure involved many compounds, such as N,2-Dihydroxybenzamide (cas: 89-73-6) .

N,2-Dihydroxybenzamide(cas: 89-73-6) has also been shown to be active against wild-type strains of Candida glabrata, but not against resistant mutants. Product Details of 89-73-6 This drug may have therapeutic potential for bone cancer and metabolic disorders such as obesity.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

COA of Formula: C6H7N3O《LEADS-FRAG: A Benchmark Data Set for Assessment of Fragment Docking Performance》 was published in 2020. The authors were Chachulski, Laura;Windshuegel, Bjoern, and the article was included in《Journal of Chemical Information and Modeling》. The author mentioned the following in the article:

Fragment-based drug design is a popular approach in drug discovery, which makes use of computational methods such as mol. docking. To assess fragment placement performance of mol. docking programs, we constructed LEADS-FRAG, a benchmark data set containing 93 high-quality protein-fragment complexes that were selected from the Protein Data Bank using a rational and unbiased process. The data set contains fully prepared protein and fragment structures and is publicly available. Moreover, we used LEADS-FRAG for evaluating the small-mol. docking programs AutoDock, AutoDock Vina, FlexX, and GOLD for their fragment docking performance. GOLD in combination with the scoring function ChemPLP and AutoDock Vina performed best and generated near-native conformations (root mean square deviation <1.5 Å) for more than 50% of the data set considering the top-ranked docking pose. Taking into account all docking poses, the tested programs generated near-native conformations for up to 86% of the fragments in LEADS-FRAG. By rescoring all docking poses with the GOLD scoring functions and the Protein-Ligand Informatics force field, the number of near-native conformations increased up to 40% with respect to the top-rescored poses. Our results show that conventional small-mol. docking programs achieve a satisfactory fragment docking performance when utilizing rescoring. The experimental procedure involved many compounds, such as 6-Aminonicotinamide (cas: 329-89-5) .

6-Aminonicotinamide (cas:329-89-5)COA of Formula: C6H7N3O is an aminopyridine, which is a specific pentose inhibitor and thus inhibits the NADP production.It can be used as a reactant for the synthesis of 6-substituted imidazo[1,2-a]pyridines with potential application as chemotherapeutic drugs.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

SDS of cas: 89-73-6《Significance of dimeric surfactant on kinetic study of organophosphorus compounds》 was published in 2022. The authors were Lakra, Jyotsna;Tikariha, Deepti;Kumar, Birendra, and the article was included in《International Journal of Chemical Kinetics》. The author mentioned the following in the article:

Dimeric surfactants have shown significant role on hydrolysis of organo-phosphorus compounds In this study, we have studied the kinetic hydrolysis of p-nitrophenyl acetate (PNPA), p-nitrophenyl benzoate (PNPB), and p-dinitrophenyl di-Ph phosphate (PNPDPP) in presence of novel dimeric surfactants 12-4-12, 2Br- (butanediyl-1,4-bis(dimethyldodecylammonium bromide)), 12-4(OH)-12, 2Br- (2-butanol-1,4-bis(dimethyldodecylammonium bromide)) with conventional nucleophiles, benzohydroxamic acid (BHA), and salicylhydroxamic acid (SHA) by spectrophotometry at 27°C. Kinetic study on effect of micelles on reaction rates have been investigated and rationalization effects of micelles determination using kinetic model. The exptl. kinetic data were fitted with micellar pseudophase model for determination micellar substrate binding and parameters. The second order rate constant is processed for comparing the reactivities in aqueous and micellar pseudophase. And N,2-Dihydroxybenzamide (cas: 89-73-6) was used in the research process.

N,2-Dihydroxybenzamide(cas: 89-73-6) is a hydroxamic acid that inhibits the activity of p-hydroxybenzoic acid (PHBA) reductase, an enzyme involved in the conversion of PHBA to benzoic acid. SDS of cas: 89-73-6 The compound has been shown to inhibit mitochondrial membrane potential and mitochondrial functions, leading to cell death.

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics