Tag: 0-100

On March 9, 2020, Yamashita, Yu; Vinogradova, Ekaterina V.; Zhang, Xiaoyu; Suciu, Radu M.; Cravatt, Benjamin F. published an article.Formula: C2H4ClNO The title of the article was A Chemical Proteomic Probe for the Mitochondrial Pyruvate Carrier Complex. And the article contained the following:

Target engagement assays are crucial for establishing the mechanism-of-action of small mols. in living systems. Integral membrane transporters can present a challenging protein class for assessing cellular engagement by small mols. The chem. proteomic discovery of alpha-chloroacetamide (αCA) compounds that covalently modify cysteine-54 (C54) of the MPC2 subunit of the mitochondrial pyruvate carrier (MPC) is presented. This finding is used to create an alkyne-modified αCA, YY4-yne, that serves as a cellular engagement probe for MPC2 in click chem.-enabled western blotting or global mass spectrometry-based proteomic experiments Studies with YY4-yne revealed that UK-5099, an alpha-cyanocinnamate inhibitor of the MPC complex, engages MPC2 with remarkable selectivity in human cells. These findings support a model where UK-5099 inhibits the MPC complex by binding to C54 of MPC2 in a covalent reversible manner that can be quantified in cells using the YY4-yne probe. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Formula: C2H4ClNO

The Article related to chem proteomic probe mitochondrial pyruvate carrier complex mcp2 uk5099, chemical proteomics, cysteine, mitochondrial pyruvate complex, target engagement, α-chloroacetamide and other aspects.Formula: C2H4ClNO

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On November 8, 2020, Lajin, Bassam; Goessler, Walter published an article.Recommanded Product: 79-07-2 The title of the article was Fluorinated carboxylic acids as “ion repelling agents” in reversed-phase chromatography. And the article contained the following:

Fluorinated carboxylic acids have been in use as ion-pairing reagents for over three decades. It has been observed that ion-pairing reagents not only increase the retention of oppositely charged analytes on reversed-phase HPLC columns but also decrease the retention of similarly charged analytes; these latter effects, however, have not been thoroughly investigated for the fluorinated carboxylic acids, and the application of these reagents has been rather restricted to their ion-pairing capacity to sep. basic analytes. In the present study, we report a systematic investigation about the effects of three fluorinated carboxylic acids (trifluoroacetic acid (TFA), pentafluoropropionic acid (PFPA), and heptafluorobutyric acid (HFBA)) on the retention and selectivity of the separation of halogenated carboxylic acids and sulfonic acids by reversed-phase chromatog. with an inductively coupled plasma mass spectrometry detector (ICPMS). Several eluents were tested and compared at different concentrations (0-100 mM) and pH values, including sulfate, nitrate, phosphate, oxalate, TFA, PFPA, and HFBA. The fluorinated carboxylic acids resulted in a consistent decrease in the retention factors (up to ca. 9-fold with HFBA) in a concentration dependent manner, which plateaued at around 50 mM. Significant improvement of the peak symmetry of the chromatographed acids was also observed We highlight the advantages of incorporating the fluorinated carboxylic acids in modifying the selectivity and retention of organic acids in reversed phase chromatog. in general, and particularly when employing chromatog. detectors with limited compatibility with organic mobile phases such as the ICPMS. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Recommanded Product: 79-07-2

The Article related to fluorinated carboxylic acid ion repelling agent reversed phase chromatog, alkyl sulfonate, fluorinated acetic acids, heptafluorobutyric acid, icpms, ion-pair chromatography and other aspects.Recommanded Product: 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On September 30, 2022, Liman, Recep; Ali, Muhammad Muddassir; Istifli, Erman Salih; Cigerci, Ibrahim Hakki; Bonciu, Elena published an article.Formula: C2H4ClNO The title of the article was Genotoxic and cytotoxic effects of pethoxamid herbicide on Allium cepa cells and its molecular docking studies to unravel genotoxicity mechanism. And the article contained the following:

Pethoxamid is chloroacetamide herbicide. Pethoxamid is commonly used to kill different weeds in various crops. Pethoxamid can leach in the water and soil and can cause toxic effects to other non-target species. Current study is therefore aimed to perform the investigation of the cytotoxic and genotoxic effects of pethoxamid on Allium cepa cells. The root growth, mitotic index (MI), chromosomal aberrations (CAs), and DNA damage were assessed through root growth inhibition, A. cepa ana-telophase, and alk. comet assays, resp. Furthermore, mol. docking was performed to evaluate binding affinity of pethoxamid on DNA and very-long-chain fatty acid (VLCFA) synthases. In root growth inhibition test, onion root length was statistically significantly decreased in a concentration dependent manner. Concentration- and time-dependent decreases in MI were observed, whereas increase in CAs such as disturbed ana-telophase, chromosome laggards, stickiness, anaphase bridges, and DNA damage was caused by the pethoxamid on A. cepa root cells. Mol. docking revealed that pethoxamid binds selectively to GC-rich regions in the minor groove of the DNA structure and showed remarkable binding affinity against all synthases taking part in the sequential biosynthesis of VLCFAs. It was concluded that the pethoxamid-induced genotoxicity and cytotoxicity may be through multiple binding ability of this herbicide with DNA and VLCFA synthases. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Formula: C2H4ClNO

The Article related to pethoxamid herbicide allium cepa cell mol docking genotoxicity, binding affinity, chromosomal aberrations, dna damage, long-chain fatty acids, mitotic index, molecular docking, pethoxamid and other aspects.Formula: C2H4ClNO

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On November 30, 2021, Yang, Jing; Chen, Xiaoqian; Yin, Daqiang; Yin, Haowen; Zhang, Ying; Liu, Min; Zhao, Weigang; Zhang, Lu; Liu, Yanan; Yang, Xichen; Zhong, Zhijie published an article.Quality Control of 2-Chloroacetamide The title of the article was Applicability of Enchytraeus bulbosus as a model species in ecotoxicology and risk assessment. And the article contained the following:

Enchytraeus bulbosus is listed in the ISO and OECD standard guidelines as a possible test species of enchytraeid. However, in contrast to other listed species, its applicability in ecotoxicol. studies as well as its sensitivity is widely unknown. Therefore, copper, pentachlorophenol(PCP), carbendazim, and chloroacetamide have been investigated by performing two-generation studies with multiple endpoints. Comparable toxicity trends to the existing studies were shown for copper and PCP in the two-generation studies of E. bulbosus. Dose-related abnormal swelling of clitella were found for the first time with PCP and chloroacetamide treatments. Sensitivity comparisons of E. bulbosus to other terrestrial test species were also conducted. E. bulbosus showed high sensitivity, it has comparable sensitivity as other sensitive species of genus Enchytraeus ( E. albidus or E. luxuriosus)to different chems., and was more sensitive than E. crypticus and earthworm species ( Eisenia fetida or Eisenia andrei). Combined with the phylogenetic and biol. characterization, the results lead to the conclusion that E.bulbosus is a suitable model species in ecotoxicol. and the chem. risk assessment (especially in multi-generation) because it has a short generation time, comparatively moderate fecundity, ideal and reasonable sensitivity. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Quality Control of 2-Chloroacetamide

The Article related to copper pentachlorophenol carbendazim chloroacetamide enchytraeus ecotoxicol risk assessment, carbendazim, chloroacetamide, copper, enchytraeus bulbosus, pentachlorophenol, two generations and other aspects.Quality Control of 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On March 31, 2020, Paronikyan, Ervand G.; Dashyan, Shushanik Sh.; Mamyan, Suren S. published an article.HPLC of Formula: 79-07-2 The title of the article was Synthesis of pyrano[3,4-c]thieno[3,2-e][1,2,4]triazolo[4,3-a]pyridines, representatives of a new fused heterocyclic system. And the article contained the following:

New effective synthesis of functionalized pyrano[3,4-c]thieno-[3,2-e][1,2,4]triazolo[4,3-a]pyridines I (Y = COOEt, C(O)NH2, CN) is described. The synthesis comprises nucleophilic substitution of 8-morpholino-6-thioxopyrano[3,4-c]pyridine-5-carbonitrile with hydrazine hydrate followed by sequential heterocyclization with formic acid and chloroacetic acid derivatives The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).HPLC of Formula: 79-07-2

The Article related to pyranothienotriazolopyridine preparation, morpholino thioxopyranopyridine carbonitrile hydrazine hydrate nucleophilic substitution, formic chloroacetic acid derivative heterocyclization and other aspects.HPLC of Formula: 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On November 30, 2020, Zhang, Nan; Hu, Xiaoling; Guan, Ping; Xu, Yarong; Liu, Zhuangjian; Cheng, Yuan published an article.Application of 79-07-2 The title of the article was Effect of surface functionality of molecularly imprinted composite nanospheres on specific recognition of proteins. And the article contained the following:

The surface functionality of biomaterial plays a primary role in determining its application in biorecognition and drug delivery. In our work, three types of synthetic tailoring polymer nanospheres with hierarchical architecture were constructed to obtain functional polymer layer with disparate chem. motifs for protein adsorption via surface imprinting and grafting copolymerization In this polymerization system, the structure stability of template protein bovine serum albumin (BSA) is well maintained within a certain range, which facilitated the accurate imprinting and precise identification. A comprehensive protocol for screening different functional layer is proposed through comparing the adsorption behavior, selectivity, identification and responsiveness to medium pH of three functional layers. Our study demonstrates that surface functionality greatly influences the adsorption capacity and selectivity of adsorption material. The functional layer with ionic liquid structure that could only provide multiple non-covalent binding sites is beneficial to the proteins aggregation and extraction, while the anti-nonspecific binding functional layer of biomaterial with zwitterionic structure for specific protein capture is promising to serve as a preferable antigen-antibody communication network, which shows great potential for protein recognition and separation In summary, our proposed strategy provides a systematic selection criterion of biomaterials for effective application in biosensors. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Application of 79-07-2

The Article related to molecularly imprinted polymer nanocomposite nanosphere surface functionalization, composite nanospheres, molecularly imprinted nanospheres, specific recognition and separation, surface functionality and other aspects.Application of 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On February 5, 2022, Han, Lingxi; Liu, Tong; Fang, Kuan; Li, Xianxu; You, Xiangwei; Li, Yiqiang; Wang, Xiuguo; Wang, Jun published an article.Electric Literature of 79-07-2 The title of the article was Indigenous functional microbial communities for the preferential degradation of chloroacetamide herbicide S-enantiomers in soil. And the article contained the following:

This study investigated indigenous functional microbial communities associated with the degradation of chloroacetamide herbicides acetochlor (ACE), S-metolachlor (S-MET) and their enantiomers in repeatedly treated soils. The results showed that biodegradation was the main process for the degradation of ACE, S-MET and their enantiomers. Eight dominant bacterial genera associated with the degradation were found: Amycolatopsis, Saccharomonospora, Mycoplasma, Myroides, Mycobacterium, Burkholderia, Afipia, and Kribbella. The S-enantiomers of ACE and S-MET were preferentially degraded, which mainly relied on Amycolatopsis, Saccharomonospora and Kribbella for the ACE S-enantiomer and Amycolatopsis and Saccharomonospora for the S-MET S-enantiomer. Importantly, the relative abundances of Amycolatopsis and Saccharomonospora increased by 146.3%-4467.2% in the S-enantiomer treatments of ACE and S-MET compared with the control, which were significantly higher than that in the corresponding R-enantiomer treatments (25.3%-4168.2%). Both metagenomic and qPCR analyses demonstrated that four genes, ppah, alkb, benA, and P 450, were the dominant biodegradation genes (BDGs) potentially involved in the preferential degradation of the S-enantiomers of ACE and S-MET. Furthermore, network anal. suggested that Amycolatopsis, Saccharomonospora, Mycoplasma, Myroides, and Mycobacterium were the potential hosts of these four BDGs. Our findings indicated that Amycolatopsis and Saccharomonospora might play pivotal roles in the preferential degradation of the S-enantiomers of ACE and S-MET. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Electric Literature of 79-07-2

The Article related to chloroacetamide enantiomer microbial community biodegradation wastewater treatment china, chloroacetamide herbicides, degradation, enantiomers, functional microbial communities, network analysis and other aspects.Electric Literature of 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On January 10, 2022, Xu, Xiaolei; Gujarati, Priyansh D.; Okwor, Neechi; Sivey, John D.; Reber, Keith P.; Xu, Wenqing published an article.Reference of 2-Chloroacetamide The title of the article was Reactivity of chloroacetamides toward sulfide + black carbon: Insights from structural analogues and dynamic NMR spectroscopy. And the article contained the following:

Chloroacetamides are commonly used in herbicide formulations, and their occurrence has been reported in soils and groundwater. However, how their chem. structures affect transformation kinetics and pathways in the presence of environmental reagents such as hydrogen sulfide species and black carbon has not been investigated. In this work, we assessed the impact of increasing Cl substituents on reaction kinetics and pathways of six chloroacetamides. The contribution of individual pathways (reductive dechlorination vs. nucleophilic substitution) to the overall decay of selected chloroacetamides was differentiated using various exptl. setups; both the transformation rates and product distributions were characterized. Our results suggest that the number of Cl substituents affected reaction pathways and kinetics: trichloroacetamides predominantly underwent reductive dechlorination whereas mono- and dichloroacetamides transformed via nucleophilic substitution. Furthermore, we synthesized eight dichloroacetamide analogs (Cl2CHC(=O)NRR′) with differing R groups and characterized their transformation kinetics. Dynamic NMR spectroscopy was employed to quantify the rotational energy barriers of dichloroacetamides. Our results suggest that adsorption of dichloroacetamides on black carbon constrained R groups from approaching the dichloromethyl carbon and subsequently favored nucleophilic attack. This study provides new insights to better predict the fate of chloroacetamides in subsurface environments by linking their structural characteristics to transformation kinetics and pathways. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Reference of 2-Chloroacetamide

The Article related to black carbon chloroacetamide transformation kinetics structural analog dynamic nmr, dechlorination, dynamic nmr spectroscopy, halogenated agrochemical fate, polysulfides, subsurface environments and other aspects.Reference of 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On June 30, 2022, Liu, Junwei; Bao, Yixuan; Zhang, Xuan; Zhao, Shiyu; Qiu, Jiguo; Li, Na; He, Jian published an article.Reference of 2-Chloroacetamide The title of the article was Anaerobic biodegradation and detoxification of chloroacetamide herbicides by a novel Proteiniclasticum sediminis BAD-10T. And the article contained the following:

Chloroacetamide herbicides (CAAHs) are important herbicides that were widely used to control agricultural weeds. However, their mass applications have seriously contaminated environment, and they are toxic to living beings. CAAHs are easy to enter anoxic environments such as subsoil, wetland sediment, and groundwater, where CAAHs are mainly degraded by anaerobic organisms. To date, there are no research on the anaerobic degradation of CAAHs by pure isolate and toxicity of anaerobic metabolites of CAAHs. In this study, the anaerobic degradation kinetics and metabolites of CAAHs by an anaerobic isolate BAD-10T and the toxicity of anaerobic metabolites were studied. Isolate BAD-10T could degrade alachlor, acetochlor, propisochlor, butachlor, pretilachlor and metolachlor with the degradation kinetics fitting the pseudo-first-order kinetics equation. The degradation rates of CAAHs were significantly affected by the length of N-alkoxyalkyl groups, the shorter the N-alkoxyalkyl groups, the higher the degradation rates. Four metabolites 2-ethyl-6-methyl-N-(ethoxymethyl)-acetanilide (EMEMA), N-(2-methyl-6-ethylphenyl)-acetamide (MEPA), N-2-ethylphenyl acetamide and 2-ethyl-N-carboxyl aniline were identified during acetochlor degradation, and an anaerobic catabolic pathway of acetochlor was proposed. The toxicity of EMEMA and EMPA for zebrafish, Arabidopsis and Chlorella ellipsoidea were obviously lower than that of acetochlor, indicating that the anaerobic degradation of acetochlor by isolate BAD-10T is a detoxification process. The work reveals the anaerobic degradation kinetics and catabolic pathway of CAAHs and highlights a potential application of Proteiniclasticum sediminis BAD-10T for bioremediation of CAAHs residue-contaminated environment. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Reference of 2-Chloroacetamide

The Article related to chloroacetamide herbicide anaerobic biodegradation detoxification, acetochlor, anaerobic biodegradation, catabolic pathway, chloroacetamide herbicides, detoxification, proteiniclasticum sediminis bad-10(t) and other aspects.Reference of 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On December 1, 2020, Verdugo, Edgard M.; Gifford, Mac; Glover, Caitlin; Cuthbertson, Amy A.; Trenholm, Rebecca A.; Kimura, Susana Y.; Liberatore, Hannah K.; Richardson, Susan D.; Stanford, Benjamin D.; Summers, R. Scott; Dickenson, Eric R. V. published an article.Application of 79-07-2 The title of the article was Controlling disinfection byproducts from treated wastewater using adsorption with granular activated carbon: Impact of pre-ozonation and pre-chlorination. And the article contained the following:

This study measured chlorine- and chloramine-reactive precursors using formation potential (FP) tests of nine U. S. Environmental Protection Agency (EPA) regulated and 57 unregulated disinfection byproducts (DBPs) in tertiary-filtered wastewater before and after pilot-scale granular activated carbon (GAC) adsorption. Using breakthrough of precursor concentration and of concentration associated calculated cytotoxicity and genotoxicity (by correlating known lethal concentrations reported elsewhere), the performance of three parallel GAC treatment trains were compared against tertiary-filtered wastewater: ozone/GAC, chlorine/GAC, and GAC alone. Results show GAC alone was the primary process, vs. ozone or chlorine alone, to remove the largest fraction of total chlorine- and chloramine-reactive DBP precursors and calculated cytotoxicity and genotoxicity potencies. GAC with pre-ozonation removed the most chlorine- and chloramine-reactive DBP precursors followed by GAC with pre-chlorination and lastly GAC without pre-treatment. GAC with pre-ozonation produced an effluent with cytotoxicity and genotoxicity of DBPs from FP that generally matched that of GAC without pre-oxidation; meanwhile removal of toxicity was greater by GAC with pre-chlorination. The cytotoxicity and genotoxicity of DBPs from FP tests did not scale with DBP concentration; for example, more than 90% of the calculated cytotoxicity resulted from 20% of the DBPs, principally from haloacetaldehydes, haloacetamides, and haloacetonitriles. The calculated cytotoxicity and genotoxicity from DBPs associated with FP-chloramination were at times higher than with FP-chlorination though the concentration of DBPs was five times higher with FP-chlorination. The removal of DBP precursors using GAC based treatment was at least as effective as removal of DOC (except for halonitromethanes for GAC without pre-oxidation and with pre-chlorination), indicating DOC can be used as an indicator for DBP precursor adsorption efficacy. However, the DOC was not a good surrogate for total cytotoxicity and genotoxicity breakthrough behavior, therefore, unregulated DBPs could have neg. health implications that are disconnected from general water quality parameters, such as DOC, and regulated classes of DBPs. Instead, cytotoxicity and genotoxicity correlate with the concentration of specific classes of unregulated DBPs. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Application of 79-07-2

The Article related to carbon pre ozonation chlorination cytotoxicity adsorption, chlorine and chloramine disinfection byproducts, cytotoxicity, genotoxicity, granular activated carbon adsorption, potable reuse, preoxidation and other aspects.Application of 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics