Tag: 0-100

On August 20, 2020, Vinogradova, Ekaterina V.; Zhang, Xiaoyu; Remillard, David; Lazar, Daniel C.; Suciu, Radu M.; Wang, Yujia; Bianco, Giulia; Yamashita, Yu; Crowley, Vincent M.; Schafroth, Michael A.; Yokoyama, Minoru; Konrad, David B.; Lum, Kenneth M.; Simon, Gabriel M.; Kemper, Esther K.; Lazear, Michael R.; Yin, Sifei; Blewett, Megan M.; Dix, Melissa M.; Nguyen, Nhan; Shokhirev, Maxim N.; Chin, Emily N.; Lairson, Luke L.; Melillo, Bruno; Schreiber, Stuart L.; Forli, Stefano; Teijaro, John R.; Cravatt, Benjamin F. published an article.Application of 79-07-2 The title of the article was An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. And the article contained the following:

Electrophilic compounds originating from nature or chem. synthesis have profound effects on immune cells. These compounds are thought to act by cysteine modification to alter the functions of immune-relevant proteins; however, our understanding of electrophile-sensitive cysteines in the human immune proteome remains limited. Here, we present a global map of cysteines in primary human T cells that are susceptible to covalent modification by electrophilic small mols. More than 3,000 covalently liganded cysteines were found on functionally and structurally diverse proteins, including many that play fundamental roles in immunol. We further show that electrophilic compounds can impair T cell activation by distinct mechanisms involving the direct functional perturbation and/or degradation of proteins. Our findings reveal a rich content of ligandable cysteines in human T cells and point to electrophilic small mols. as a fertile source for chem. probes and ultimately therapeutics that modulate immunol. processes and their associated disorders. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Application of 79-07-2

The Article related to electrophile cysteine interaction primary human t cell immunol, birc3, itk, t cells, activity-based protein profiling, chemical proteomics, covalent, cysteine, electrophiles, human, protein degradation and other aspects.Application of 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On August 31, 2020, Sanad, Sherif M. H.; Mekky, Ahmed E. M. published an article.Recommanded Product: 2-Chloroacetamide The title of the article was Piperazine-mediated tandem synthesis of bis(thieno[2,3-b]pyridines): Versatile precursors for related fused [1,2,4]triazolo[4,3-a]pyrimidines. And the article contained the following:

In this study, the utility of bis(cyanoacetamides) as versatile precursors to the piperazine-mediated synthesis of a wide spectrum of bis(thieno[2,3-b]pyridine) derivatives, linked to aliphatic spacers via thioethers was reported. The proposed tandem protocol involved the reaction of bis(cyanoacetamides) with two equivalent of the appropriate cinnamonitriles in dioxane in the presence of six equivalent of piperazine was refluxed for 4 h which on further addition of halogen-containing reagents afforded bis(thieno[2,3-b]pyridine) derivatives I [Ar = 4-MeOC6H4, 4-ClC6H4; Y = CN, C(O)Me, C(O)NH2, etc.; Z = CH2, (CH2)3]. Compounds I were taken as a key intermediates and reacted with formic acid/acetic anhydride/carbon disulfide afforded bis(oxopyrido[3′,2′:4,5]thieno[3,2-d]pyrimidine) derivatives II [R = H, Me; R1 = CH2, (CH2)3; Ar1 = 4-MeOC6H4, 4-ClC6H4] and III [R2 = CH2, (CH2)3]. Compounds III were reacted with the appropriate hydrazonyl chloride derivatives in dioxane in the presence of triethylamine afforded the corresponding bis([1,2,4]triazoles) with related fused pyridothienopyrimidine moiety IV [R3 = CH2, (CH2)3; Ar2 = Ph, 4-MeC6H4, 4-ClC6H4]. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Recommanded Product: 2-Chloroacetamide

The Article related to bis thienopyridine preparation, cyanoacetamide cinnamonitrile halogen reagent tandem four component piperazine mediated, oxopyridothienopyrimidine bis preparation, acetyl pyridothienotriazolopyrimidine bis preparation and other aspects.Recommanded Product: 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On August 16, 2022, Liang, Chao; Zhang, Shan; Robinson, David; Ploeg, Matthew Vander; Wilson, Rebecca; Nah, Jiemin; Taylor, Dale; Beh, Sheryl; Lim, Radiance; Sun, Lei; Muoio, Deborah M.; Stroud, David A.; Ho, Lena published an article.Product Details of 79-07-2 The title of the article was Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis. And the article contained the following:

Electron transport chain (ETC) biogenesis is tightly coupled to energy levels and availability of ETC subunits. Complex III (CIII), controlling ubiquinol:ubiquinone ratio in ETC, is an attractive node for modulating ETC levels during metabolic stress. Here, we report the discovery of mammalian Co-ordinator of mitochondrial CYTB (COM) complexes that regulate the stepwise CIII biogenesis in response to nutrient and nuclear-encoded ETC subunit availability. The COMA complex, consisting of UQCC1/2 and membrane anchor C16ORF91, facilitates translation of CIII enzymic core subunit CYTB. Subsequently, microproteins SMIM4 and BRAWNIN together with COMA subunits form the COMB complex to stabilize nascent CYTB. Finally, UQCC3-containing COMC facilitates CYTB hemylation and association with downstream CIII subunits. Furthermore, when nuclear CIII subunits are limiting, COMB is required to chaperone nascent CYTB to prevent OXPHOS collapse. Our studies highlight CYTB synthesis as a key regulatory node of ETC biogenesis and uncover the roles of microproteins in maintaining mitochondrial homeostasis. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Product Details of 79-07-2

The Article related to respiratory chain biogenesis mitochondrial microprotein homeostasis, cp: metabolism, cytb, seps, smim4, uqcc1, uqcc2, complex iii, electron transport chain, microproteins, nuclear-mitochondrial coordination, smorfs and other aspects.Product Details of 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On June 20, 2022, Wang, Zheng; Liao, Yufeng; Li, Xiuwen; Shuang, Chendong; Pan, Yang; Li, Yan; Li, Aimin published an article.Recommanded Product: 2-Chloroacetamide The title of the article was Effect of ammonia on acute toxicity and disinfection byproducts formation during chlorination of secondary wastewater effluents. And the article contained the following:

Ammonia nitrogen (NH3-N) significantly affects the occurrence of disinfection byproducts (DBPs) and residual chlorine in chlorinated wastewater, thereby affecting the acute toxicity to aquatic organisms. In this paper, the formation of thirty-five halogenated DBPs and the changes in acute toxicity of luminescent bacteria and zebrafish embryos were evaluated after chlorination of seven secondary wastewater effluents with different NH3-N concentrations Results showed that NH3-N significantly reduced the formation of most DBPs by 82-100%. The acute toxicity was enhanced after chlorination and increased linearly with increasing NH3-N concentration for luminescent bacteria (r = 0.986, p < 0.05) and zebrafish embryos (r = 0.972, p < 0.05) due to the coexistence of DBPs and monochloramine. According to the toxicity classification system of wastewater, the fitting results indicated that the toxicity level was acceptable for chlorinated wastewater with NH3-N concentration below 1.00 mg-N/L. DBPs might be the main toxicant to luminescent bacteria in the wastewater with low NH3-N concentrations (0.06-0.31 mg-N/L), which accounted for 68-97% of the toxicity contribution. By contrast, monochloramine contributed over 80% to the toxicity of luminescent bacteria and zebrafish embryos in the wastewater with high NH3-N concentrations (2.66-7.17 mg-N/L). Compared to chlorination, chlorine dioxide and UV disinfection unaffected by NH3-N could reduce acute toxicity by nearly 100%, primarily due to the lack of residual disinfectant. In view of the high toxicity caused by chlorination, chlorination-dechlorination or chlorine dioxide and UV disinfection are highly recommended for the treatment of wastewater with high NH3-N concentration The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Recommanded Product: 2-Chloroacetamide

The Article related to ammonia acute toxicity disinfection byproduct secondary wastewater effluent chlorination, alternative disinfection process, ammonia-containing wastewater, dechlorination, residual chlorine, toxicity contribution and other aspects.Recommanded Product: 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On November 1, 2020, Qian, Yunkun; Hu, Yue; Chen, Yanan; An, Dong; Westerhoff, Paul; Hanigan, David; Chu, Wenhai published an article.SDS of cas: 79-07-2 The title of the article was Haloacetonitriles and haloacetamides precursors in filter backwash and sedimentation sludge water during drinking water treatment. And the article contained the following:

Haloacetonitriles (HANs) and haloacetamides (HAMs) are nitrogenous disinfection byproducts that are present in filter backwash water (FBW) and sedimentation sludge water (SSW). In many cases FBW and SSW are recycled to the head of drinking water treatment plants. HAN and HAM concentrations in FBW and SSW, without addnl. oxidants, ranged from 6.8 to 11.6 nM and 2.9 to 3.6 nM of three HANs and four HAMs, resp. Upon oxidant addition to FBW and SSW under formation potential conditions, concentrations for six HANs and six HAMs ranged from 92.2 to 190.4 nM and 42.2 to 95.5 nM, resp. Therefore, at common FBW and SSW recycle rates (2 to 10% of treated water flows), the precursor levels in these recycle waters should not be ignored because they are comparable to levels present in finished water. Brominated HAN and chlorinated HAM were the dominant species in FBW and SSW, resp. The lowest mol. weight ultrafiltration fraction (< 3 kDa) contributed the most to HAN and HAM formations. The hydrophilic (HPI) organic fraction contributed the greatest to HAN precursors in sand-FBW and SSW and were the most reactive HAM precursors in both sand- or carbon-FBWs. Fluorescence revealed that aromatic protein-like compounds were dominant HAN and HAM precursors. Therefore, strategies that remove low mol. weight hydrophilic organic matter and aromatic protein-like compounds will minimize HAN and HAM formations in recycled FBW and SSW. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).SDS of cas: 79-07-2

The Article related to haloacetonitrile haloacetamide filter backwash sedimentation sludge water, drinking water treatment, filter backwash water, haloacetamides, disinfection byproducts, haloacetonitriles, sedimentation sludge water and other aspects.SDS of cas: 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On August 16, 2021, Kuskov, Andrey; Selina, Oxana; Kulikov, Pavel; Imatdinov, Ilnaz; Balysheva, Vera; Kryukov, Alexander; Shtilman, Mikhail; Markvicheva, Elena published an article.Recommanded Product: 2-Chloroacetamide The title of the article was Amphiphilic Poly(N-Vinylpyrrolidone) Nanoparticles Loaded with DNA Plasmids Encoding Gn and Gc Glycoproteins of the Rift Valley Fever Virus: Preparation and In Vivo Evaluation. And the article contained the following:

The aim of the study was to develop amphiphilic poly(N-vinylpyrrolidone) (PVP) nanoparticles (NPs) loaded with DNA plasmids encoding Gn and Gc glycoproteins of the Rift Valley fever virus (RVFV) and to study the humoral response in vivo. DNA plasmids were protected from extracellular nucleases by loading in NPs from PVP derivatives modified with amino acids β-alanine (Ala7-PVPOD4000) or glycine (Gly7.5-PVP-OD4000) fabricated by the original self-assembly technique. The obtained NPs were administered in mice and the enhancement of humoral response compared to this one in case of immunization with native DNA plasmids was demonstrated. The NPs loaded with DNA plasmids are promising for the fabrication of various DNA particulate vaccines. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Recommanded Product: 2-Chloroacetamide

The Article related to alanine glycine modified polyvinylpyrrolidone nanoparticle fabrication self assembly cytotoxicity, dna vaccines, gn and gc glycoproteins, rift valley fever virus, amphiphilic polymers, humoral response, nanoparticles, poly(n-vinylpyrrolidone) and other aspects.Recommanded Product: 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On February 20, 2020, Klann, Kevin; Tascher, Georg; Muench, Christian published an article.Application In Synthesis of 2-Chloroacetamide The title of the article was Functional Translatome Proteomics Reveal Converging and Dose-Dependent Regulation by mTORC1 and eIF2α. And the article contained the following:

Regulation of translation is essential during stress. However, the precise sets of proteins regulated by the key translational stress responses-the integrated stress response (ISR) and mTORC1-remain elusive. We developed multiplexed enhanced protein dynamics (mePROD) proteomics, adding signal amplification to dynamic-SILAC and multiplexing, to enable measuring acute changes in protein synthesis. Treating cells with ISR/mTORC1-modulating stressors, we showed extensive translatome modulation with ∼20% of proteins synthesized at highly reduced rates. Comparing translation-deficient sub-proteomes revealed an extensive overlap demonstrating that target specificity is achieved on protein level and not by pathway activation. Titrating cap-dependent translation inhibition confirmed that synthesis of individual proteins is controlled by intrinsic properties responding to global translation attenuation. This study reports a highly sensitive method to measure relative translation at the nascent chain level and provides insight into how the ISR and mTORC1, two key cellular pathways, regulate the translatome to guide cellular survival upon stress. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Application In Synthesis of 2-Chloroacetamide

The Article related to proteomics translatome mtorc1 eif2alpha protein dynamics integrated stress response, silac, tmt, cap-dependent translation, integrated stress response, mtor, proteomics, pulse labeling, stress response, translation, unfolded protein response and other aspects.Application In Synthesis of 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Mohi El-Deen, Eman M.; Anwar, Manal M.; El-Gwaad, Amina A. Abd; Karam, Eman A.; El-Ashrey, Mohamed K.; Kassab, Rafika R. published an article in 2022, the title of the article was Novel Pyridothienopyrimidine Derivatives: Design, Synthesis and Biological Evaluation as Antimicrobial and Anticancer Agents.COA of Formula: C2H4ClNO And the article contains the following content:

The growing risk of antimicrobial resistance besides the continuous increase in the number of cancer patients represents a great threat to global health, which requires intensified efforts to discover new bioactive compounds to use as antimicrobial and anticancer agents. Thus, a new set of pyridothienopyrimidine derivatives 2a,b-9a,b was synthesized via cyclization reactions of 3-amino-thieno[2,3-b]pyridine-2-carboxamides 1a,b with different reagents. All new compounds were evaluated against five bacterial and five fungal strains. Many of the target compounds showed significant antimicrobial activity. In addition, the new derivatives were further subjected to cytotoxicity evaluation against HepG-2 and MCF-7 cancer cell lines. The most potent cytotoxic candidates (3a, 4a, 5a, 6b, 8b and 9b) were examined as EGFR kinase inhibitors. Mol. docking study was also performed to explore the binding modes of these derivatives at the active site of EGFR-PK. Compounds 3a, 5a and 9b displayed broad spectrum antimicrobial activity with MIC ranges of 4-16 μg/mL and potent cytotoxic activity with IC50 ranges of 1.17-2.79 μM. In addition, they provided suppressing activity against EGFR with IC50 ranges of 7.27-17.29 nM, higher than that of erlotinib, IC50 = 27.01 nM. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).COA of Formula: C2H4ClNO

The Article related to pyridothienopyrimidine derivative biol evaluation antimicrobial anticancer agent, egfr-pk inhibition, hepg-2 cells, mcf-7 cells, antimicrobial activity, cyclization reactions, molecular docking, pyridothienopyrimidines, thieno[2,3-b]pyridine and other aspects.COA of Formula: C2H4ClNO

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On January 31, 2020, Miao, Xiuqi; Xing, Lingyun; Guo, Ming; Zhang, Hong; Liu, Sicong; Yin, Shiliang; Gong, Ping; Zhang, Dajun; Zhai, Xin published an article.Application In Synthesis of 2-Chloroacetamide The title of the article was Design, synthesis and biological evaluation of 2-arylaminopyrimidine derivatives bearing 1,3,8-triazaspiro[4,5]decan-4-one or piperidine-3-carboxamide moiety as novel Type-I1/2 ALK inhibitors. And the article contained the following:

Aiming to develop novel Type-I1/2 inhibitors of ALK to overcome extensive resistance mutations, especially the L1196M mutation surrounding the ATP pocket, two series of 2-arylaminopyrimidine derivatives (I (R1 = pyrrolidin-1-yl, piperidin-1-yl, 4-methylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl), II (R2 = 4-methylpiperazin-1-yl, 4-(2-hydroxyethyl)piperazin-1-yl) and III (R3 = ethoxycarbonylmethylamino, 2-(piperidin-1-yl)acetamido, 2-(4-methylpiperazin-1-yl)acetamido, etc.), IV (R4 = allyl, cyclopropanecarbonyl, 2-carboxyethyl, etc.)) were designed based on scaffold hopping. The extensive structural elaboration discovered compound IV ((A), R4 = 3-ethoxy-3-oxopropyl) which possessed excellent IC50 values of 0.06 and 0.23μM against ALK-pos. Karpas299 and H2228 cell lines, resp. Meanwhile, (A) displayed encouraging inhibitory potency in the ALKWT (2.5 nM) and ALKL1196M (6.5 nM) enzymic assays. Furthermore, the AO/EB and Hoechst 33258 assays illustrated that (A) could induce cell apoptosis in a dose-dependent manner. Eventually, the mol. docking of (A) with ALK clearly presented the vital interactions within the active site, which was in accordance with Type-I1/2 inhibitor binding mode. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Application In Synthesis of 2-Chloroacetamide

The Article related to arylaminopyrimidine triazaspirodecanone preparation alk inhibitor antitumor docking, piperidine carboxamide arylaminopyrimidine preparation alk inhibitor antitumor docking, imidazolidin-2-one, l1196m mutants, piperidine-3-carboxamide, type-i(1/2) alk inhibitor and other aspects.Application In Synthesis of 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On January 31, 2020, Doellinger, Joerg; Schneider, Andy; Hoeller, Marcell; Lasch, Peter published an article.Application of 79-07-2 The title of the article was Sample preparation by easy extraction and digestion (SPEED) – a universal, rapid, and detergent-free protocol for proteomics based on acid extraction. And the article contained the following:

The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a manner that enables efficient protein digestion for subsequent mass spectrometric anal. Today’s sample preparation strategies are commonly conceptualized around the removal of detergents, which are essential for extraction but strongly interfere with digestion and LC-MS. These multi-step preparations contribute to a lack of reproducibility as they are prone to losses, biases and contaminations, while being time-consuming and labor-intensive. We report a detergent-free method, named Sample Preparation by Easy Extraction and Digestion (SPEED), which consists of three mandatory steps, acidification, neutralization and digestion. SPEED is a universal method for peptide generation from various sources and is easily applicable even for lysis-resistant sample types as pure trifluoroacetic acid (TFA) is used for highly efficient protein extraction by complete sample dissolution The protocol is highly reproducible, virtually loss-less, enables very rapid sample processing and is superior to the detergent/chaotropic agent-based methods FASP, ISD-Urea and SP3 for quant. proteomics. SPEED holds the potential to dramatically simplify and standardize sample preparation while improving the depth of proteome coverage especially for challenging samples. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Application of 79-07-2

The Article related to escherichia staphylococcus hela proteomics acid extraction speed, tfa, automation, bacteria, detergent-free, digestion, label-free quantification, lysis, mass spectrometry, microbiome, pathogens, protein denaturation, proteomics, sample preparation and other aspects.Application of 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics