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Effects of non-euphoric plant cannabinoids on muscle quality and performance of dystrophic mdx mice was written by Iannotti, Fabio Arturo;Pagano, Ester;Moriello, Aniello Schiano;Alvino, Filomena Grazia;Sorrentino, Nicolina Cristina;D’Orsi, Luca;Gazzerro, Elisabetta;Capasso, Raffaele;De Leonibus, Elvira;De Petrocellis, Luciano;Di Marzo, Vincenzo. And the article was included in British Journal of Pharmacology in 2019.Recommanded Product: 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid This article mentions the following:

We explored the possibility of using non-euphoric compounds present in Cannabis sativa, cannabidiol (CBD), cannabidivarin (CBDV) and tetrahydrocannabidivarin (THCV), to reduce inflammation, restore functional autophagy and pos. enhance muscle function in vivo. Exptl. Approach : Using quant. PCR, western blots and [Ca²⁺]_i measurements, we explored the effects of CBD and CBDV on the differentiation of both murine and human skeletal muscle cells as well as their potential interaction with TRP channels. After treatment, locomotor tests and biochem. analyses were used to evaluate their effects on inflammation and autophagy. Key Results : CBD and CBDV promoted the differentiation of murine C2C12 myoblast cells into myotubes by increasing [Ca²⁺]_i mostly via TRPV1 activation, an effect that undergoes rapid desensitization. In primary satellite cells and myoblasts isolated from healthy and/or DMD donors, not only CBD and CBDV but also THCV promoted myotube formation, in this case, mostly via TRPA1 activation. In mdx mice, CBD (60 mg·kg^-1) and CBDV (60 mg·kg^-1) prevented the loss of locomotor activity, reduced inflammation and restored autophagy. Conclusion and Implications : We provide new insights into plant cannabinoid interactions with TRP channels in skeletal muscle, highlighting a potential opportunity for novel co-adjuvant therapies to prevent muscle degeneration in DMD patients. In the experiment, the researchers used many compounds, for example, 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8Recommanded Product: 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid).

2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. The presence of the amide group –C(=O)N– is generally easily established, at least in small molecules. It can be distinguished from nitro and cyano groups in IR spectra. Amides exhibit a moderately intense νCO band near 1650 cm−1. By 1H NMR spectroscopy, CONHR signals occur at low fields. In X-ray crystallography, the C(=O)N center together with the three immediately adjacent atoms characteristically define a plane.Recommanded Product: 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Chernykh, Anton V. published the artcileA stereochemical journey around spirocyclic glutamic acid analogs, Name: (S)-2-Methylpropane-2-sulfinamide, the main research area is glutamic acid spirocyclic analog library enantioselective diastereoselective regioselective synthesis; silylated hydroxymethyl cyclobutanone Tebbe olefination; dichloroketene addition Meinwald oxirane rearrangement ketone Strecker reaction; chiral auxiliary Ellman sulfinamide glutamic acid; crystal structure spirocycle Helicobacter pylori glutamate racemase inhibitor.

A practical divergent synthetic approach is reported for the library of regio- and stereoisomers of glutamic acid analogs built on the spiro[3.3]heptane scaffold. Formation of the spirocyclic scaffold was achieved starting from a common precursor – an O-silylated 2-(hydroxymethyl)cyclobutanone derivative Its olefination required using the titanium-based Tebbe protocol since the standard Wittig reaction did not work with this particular substrate. The construction of the second cyclobutane ring of the spirocyclic system was achieved through either subsequent dichloroketene addition or Meinwald oxirane rearrangement as the key synthetic steps, depending on the substitution patterns in the target compounds (1,6- or 1,5-, resp.). Further modified Strecker reaction of the resulting racemic spirocyclic ketones with the Ellman′s sulfinamide as a chiral auxiliary had low to moderate diastereoselectivity; nevertheless, all stereoisomers were isolated in pure form via chromatog. separation, and their absolute configuration was confirmed by X-ray crystallog. Members of the library were tested for the inhibitory activity against H. pylori glutamate racemase.

Organic & Biomolecular Chemistry published new progress about Chiral auxiliary. 343338-28-3 belongs to class amides-buliding-blocks, name is (S)-2-Methylpropane-2-sulfinamide, and the molecular formula is C4H11NOS, Name: (S)-2-Methylpropane-2-sulfinamide.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On July 31, 2022, Sainz-Diaz, C. Ignacio; de la Luz, Alexander Perez; Barrientos-Salcedo, Carolina; Francisco-Marquez, Misaela; Soriano-Correa, Catalina published an article.Quality Control of N-((4-Aminophenyl)sulfonyl)acetamide The title of the article was Crystal polymorphism and spectroscopical properties of sulfonamides in solid state by means of First Principles calculations. And the article contained the following:

Sulfonamides are an important class of therapeutic agents. The increase in the number of new sulfonamide derivatives makes it necessary to study more rationally the chem. structure, because the solid forms often display different mech., thermal and physicochem. properties that can influence the bioavailability and stability of the drugs; consequently, the polymorphic structures are of great interest to the pharmaceutical industry because of their ability to modify the phys. properties of the active pharmaceutical ingredient. The mol. interactions of these drugs in their crystal lattice are important for the stability of the crystals and polymorphism and for preparing composite complexes for optimizing the use of these drugs. In this work, the crystal structure of these drugs and crystal polymorphism is investigated. So, the crystal forms of antibiotics derivatives of the sulfonamides, sulfamethoxazole, sulfamethazine, sulfachloropyridazine, and sulfacetamide are studied at the mol. and supramol. level by using computational modeling approach at quantum mech. level. The spectroscopic properties of these systems are also studied explaining assignments of previous exptl. data. The results of DFT calculations reproduce the crystal structures of sulfonamides determined exptl. and the polymorphism in these mols. have been clarified. Likewise, the main intermol. interactions in all crystal forms of these sulfonamides are H-bonds among the sulfonic and amino groups and SNH groups, and also some π-π interactions. Also, these 3-D periodical models allow the exploration of the intermol. interactions included in the crystal structures and some of these interactions can alter the vibration modes of the mols. Therefore, the use of these models can be useful for exptl. spectroscopy studies where use actual crystal solids. The experimental process involved the reaction of N-((4-Aminophenyl)sulfonyl)acetamide(cas: 144-80-9).Quality Control of N-((4-Aminophenyl)sulfonyl)acetamide

The Article related to sulfonamide solid state crystal polymorphism spectroscopy, dft calculations, sulfonamides, hydrogen bonds, infrared spectroscopy, polymorphism, Placeholder for records without volume info and other aspects.Quality Control of N-((4-Aminophenyl)sulfonyl)acetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On August 31, 2020, Kim, Yoseop; Yeo, Injoon; Kim, Hyunsoo; Son, Minsoo; Kim, Youngsoo published an article.Quality Control of 2-Chloroacetamide The title of the article was Preparation of Tissue Samples for Large-scale Quantitative Mass Spectrometric Analysis. And the article contained the following:

Tissues contain more tumor-type specific information than biofluids, such as blood, rendering them valuable resources for biomarker studies. However, considering the characteristics of tissue homogenization, it is difficult to obtain reproducible samples and analyze many samples simultaneously. To address these issues, we developed a robust and reproducible method for preparing tissues for targeted proteomics-multiple reaction monitoring-mass spectrometry (MRM-MS)-using a Bioruptor Pico sonicator. This approach uses sodium deoxycholate (SDC) as a detergent and can extract proteins from up to 20 mg of tissue using a lysis buffer volume of 300μL and a sonication time of 30 s, with 30 on/off cycles. The tryptic digestion was optimized as follows: digestion base buffer, ammonium bicarbonate (ABC); reduction and alkylation reagent, dithiothreitol (DTT) and iodoacetamide (IAA), resp.; and trypsin amount and incubation time, 1:50 (enzyme: substrate) and 10 h, resp. With regard to reproducibility, the intra-assay and inter-assay CVs for the target peptides were less than 20% (intra-CV, 0.87% to 19.13%; inter-CV, 2.3% to 13.62%). Our method was robust and reproducible in the quant. anal. of tissue by MRM-MS, rendering it applicable to the large-scale study of tissue-based biomarkers. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Quality Control of 2-Chloroacetamide

The Article related to tissue sample mass spectrometric analysis, Biochemical Methods: Spectral and Related Methods and other aspects.Quality Control of 2-Chloroacetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Hou, Qin-zheng;Wang, Yu-pei;Fan, Bao-qiang;Sun, Kun;Liang, Jun-yu;Feng, Han-qing;Jia, Ling-yun published 《Extracellular ATP affects cell viability, respiratory O₂ uptake, and intracellular ATP production of tobacco cell suspension culture in response to hydrogen peroxide-induced oxidative stress》 in 2020. The article was appeared in 《Biologia (Cham, Switzerland)》. They have made some progress in their research.Synthetic Route of C7H7NO3 The article mentions the following:

Abstract: Extracellular ATP (ATP) is a well-known signalling mol. in plants and plays important roles during plant stress response. In the present work, the treatment of tobacco cell suspension culture with exogenous hydrogen peroxide (H₂O₂) caused the decreases of respiratory O₂ uptake, intracellular ATP production, extracellular ATP level, and reduction of cell viability. Combining this observation with the finding that the oxidative phosphorylation uncoupler or the respiratory inhibitor not only decreased intracellular ATP production but also decreased the extracellular ATP level and cell viability, it is suggested that the decrease of extracellular ATP level and cell viability under H₂O₂ stress could be a result of the suppressed production of intracellular ATP. Treatment with ATP-degrading enzyme, apyrase, also caused the decreases of cell viability, respiratory O₂ uptake, and intracellular ATP production More importantly, addition of exogenous ATP alleviated the H₂O₂-induced decreases of cell viability, respiratory O₂ uptake, and production of intracellular ATP. These results indicate that extracellular ATP could be an important effector in regulating the cell viability, respiratory O₂ uptake, and intracellular ATP production of tobacco cell suspension culture under H₂O₂ stress.N,2-Dihydroxybenzamide (cas: 89-73-6) were involved in the experimental procedure.

N,2-Dihydroxybenzamide(cas: 89-73-6) can be used:To prepare phenylboronic acid-based bioconjugates for chromatographic applications;As a ligand to synthesize Fe(III), Cu(II), Ni(II) and Zn(II) complexes.Synthetic Route of C7H7NO3

Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Electric Literature of 2564-07-0, The chemical industry reduces the impact on the environment during synthesis 2564-07-0, name is 2-Chloro-N-(2,3-dimethylphenyl)acetamide, I believe this compound will play a more active role in future production and life.

The procedure described in Example 8 was followed, substituting the product from Example 17A for N-chloroacetyl-3-nitroaniline, to provide the title compound (32% yield as a white solid. mp 124-126 C.; 1H NMR (300 MHz, DMSO-d6) ? 2.12 (s, 3H), 2.26 (s, 3H), 2.72 (m, 4H), 3.21 (s, 2H), 3.69 (m, 4H), 6.94 (dd, 1H, J=7.8, 4.8 Hz), 6.99 (br d, 1H, J=7.4 Hz), 7.07 (dd, 1H, J=7.4, 7.4 Hz), 7.45 (br d, 1H, J=7.8 Hz), 8.08 (dd, 1H, J=7.8, 2.0 Hz), 8.42 (dd, 1H, 4.8, 2.1 Hz), 9.42 (br s, 1H); MS (DCI/NH3) m/e 350 (M+H)+; Anal. calcd for C20H23N50.0.10H2O: C, 68.39; H, 6.66; N, 19.94. Found: C, 68.74; H, 6.58; N, 19.56.

In the field of chemistry, the synthetic routes of compounds are constantly being developed and updated. I will also mention this compound in other articles, 2-Chloro-N-(2,3-dimethylphenyl)acetamide, other downstream synthetic routes, hurry up and to see.

Reference:
Patent; Stewart, Andrew O.; Kolasa, Teodozyj; US2003/232836; (2003); A1;,
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Reference of 104060-23-3, In the next few decades, the world population will flourish. As the population grows rapidly and people all over the world use more and more resources, all industries must consider their environmental impact. 104060-23-3, name is N-Boc-2-(4-Aminophenyl)ethanol belongs to amides-buliding-blocks compound, it is a common compound, a new synthetic route is introduced below.

Example 4: Preparation of compounds according to the invention.Diphenylphosphonylated compounds according to the invention (as e.g. in example 2) were prepared according to Scheme 1 , by a fast and convenient synthetic route, affording the opportunity to obtain large and diverse series of compounds. The aminophosphonate building block was prepared from fe/t-butylcarbamate protected 4- aminophenylacetaldehyde (12), prepared from the corresponding alcohol (11) with Dess- Martin periodinane (Dess, D. B. and Martin, J. C. Readily Accessible 12-1-5 Oxidant for the Conversion of Primary and Secondary Alcohols to Aldehydes and Ketones. J. Org. Chem., 1983, 48, 4155-4156.). An amidoalkylation reaction with benzylcarbamate and triphenylphosphite using copper triflate as catalyst, afforded lambda/-benzyloxycarbonyl protected diphenyl phosphonate 13 (Van der Veken, P.; El Sayed, I.; Joossens, J.; Stevens, C. V.; Augustyns, K. and Haemers, A.; Lewis Acid Catalyzed Synthesis of N- Protected Diphenyl 1-Aminoalkyl-Phosphonates. Synthesis, 2005, 4, 634-638). Acidolysis removed the terf-butyl carbamate protecting group. lambda/,lambda/’-bis(te/-butoxycarbonyl)-1- guanylpyrazole was used to introduce the protected guanidine group (Drake, B.; Patek, M.; Lebl, M., A Convenient Preparation of Monosubstituted N,N’-Di(Boc)-Protected Guanidines. Synthesis, 1994, 579-582.). Compound 14 was subsequently deprotected under hydrogenolytic conditions. Small non-peptide guanidyl compounds (7) were made by coupling compound (15) with selected sulfonyl chlorides or acylchlorides in pyridine. EPO The p-acetylaminophenyl phosphonates (as e.g. in example 3) were prepared following scheme 1 using tri-p-acetylaminophenylphosphite (Belyaev et al. A. Structure-Activity Relationship of Diaryl Phosphonate Esters as Potent Irreversible Dipeptidyl Peptidase IV Inhibitors. J.Med. Chem., 1999, 42, 1041-1052) (22).4-(tert-Butyloxycarbonylamino)phenylacetaldehyde (intermediate No. 12 in scheme1 ).Yield: 58%1H NMR (CDCI3, 400MHz) delta 1.5 (s, 9H)1 3.75 (d, 2H), 7.10 (d, 2H), 7.30 (m, 2H), 9.6 (t, 1 H)MS (ESI): m/z (M+Na)258, (M+Me0H+Na) 290Dess-Martin Oxidation: To a stirred suspension of alcohol (1 eq.) in DCM (80ml) at -78 0C, a solution of Dess-Martin periodane (1.5 eq from a 15wt% solution) was added. The suspension was stirred for 3h at room temperature. The resulting solution was poured into a vigorously stirred saturated NaHCO3 and Na2S2O3 solution (1 :1 100ml). The organic layer was separated and washed with brine and dried over Na2SO4. The crude product was obtained by removing the solvent in vacuo.tert-Butyl 4-(2-oxoethyl)phenylcarbamate (12)2-(4-Aminophenyl)ethanol (13.7 g, 0.1 mol) was dissolved in dioxane (120 ml). Triethylamine (10.1 g, 0.1 mol) was added followed by addition of Boc2O (21.8 g, 0.1 mol). The reaction mixture was stirred overnight. After evaporation under vacuum, the residue EPO was dissolved in ethylacetate and washed with HCI (2N) and brine. The organic layer was dried over Na2SO4 and evaporated. Purification by flash chromatography afforded the tert- butyl 4-(2-hydroxyethyl)phenylcarbamate as a white solid (19 g, 80 mmol, 80%). To a stirred solution of this alcohol (1eq) in dichloromethane, a solution of Dess-Martin periodane (1.2 eq from 15% wt solution) was added. The suspension was stirred for 4 h at room temperature. The resulting solution was poured into a vigorously stirred saturated solution of NaHCO3 and Na2S2O3 (1 : 1, 100 ml). The organic layer was separated and washed with brine and dried over anhydrous Na2SO4. This crude aldehyde 12 was used directly for further reaction.

The synthetic route of N-Boc-2-(4-Aminophenyl)ethanol has been constantly updated, and we look forward to future research findings.

Reference:
Patent; UNIVERSITEIT ANTWERPEN; WO2007/45496; (2007); A1;,
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Adding a certain compound to certain chemical reactions, such as: 16313-66-9, name is 2-Amino-5-bromobenzamide, belongs to amides-buliding-blocks compound, can increase the reaction rate and produce products with better performance than those obtained under traditional synthetic methods. Here is a downstream synthesis route of the compound 16313-66-9, category: amides-buliding-blocks

General procedure: A dry 50 mL flask was charged with 2-aminobenzamide 1 (1.1 mmol), 5,5-dimethyl-1,3-cyclohexanedione 2 (1.0 mmol), iodine (0.026 g, 0.01 mmol) and toluene (10.0 mL). The mixture was stirred at 80 C until the reactant 1 was consumed. Then, another equivalent of 2-aminobenzamide was added to the mixture, and refluxed for a few hours. After the completion of the reaction monitored by TLC, toluene was recovered by distillation under reduced pressure, and the residue was purified by silica column chromatography using ethyl acetate and petroleum ether (1:2) as the eluent to give products 6.

If you are interested in these compounds, you can also browse my other articles.Thank you for taking the time to read this article. I hope you enjoyed it.

Reference:
Article; Lu, Lian; Zhang, Mei-Mei; Jiang, Hong; Wang, Xiang-Shan; Tetrahedron Letters; vol. 54; 8; (2013); p. 757 – 760;,
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

In the chemical reaction process, reaction time, type of solvent, can easily affect the result of the reaction, thereby determining the yield and properties of the reaction product. An updated downstream synthesis route of 15351-42-5 as follows. COA of Formula: C14H10N2O2

After 20 g (0.13 mol) of methyl 2-aminobenzoate was put into a 500 mL of flask and then dissolved with THF, 6 g (0.26 mol) of sodium hydride was added thereto. The resulting mixture was stirred at about 50 C. for about 48 hours, 0.1M HC1 was added thereto to terminate the reaction, followed by extraction with dichlorimethane. The resulting organic layer was separated, and dried under reduced pressure to remove the solvent. The residue was washed with water and methanol, and dried for about 24 hours to obtain 23 g of Intermediate 18-(1) (Yield: 75%).

According to the analysis of related databases, 15351-42-5, the application of this compound in the production field has become more and more popular.

Reference:
Patent; Samsung Display Co., Ltd; Gyeongsang National University cademic Cooperation; Kim, Mi Gyoung; Kim, Yun Hee; Kwon, Sungi; Chu, Chang Ung; (138 pag.)KR2015/142709; (2015); A;,
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics