Wang, Xiaoran published the artcileAquaporin 4 differentially modulates osmotic effects on vasopressin neurons in rat supraoptic nucleus, Name: N-(1,3,4-Thiadiazol-2-yl)nicotinamide, the publication is Acta Physiologica (2021), 232(3), e13672, database is CAplus and MEDLINE.
Aim : Glial fibrillary acidic protein (GFAP) molecularly associates with aquaporin 4 (AQP4) in astrocytic plasticity. Here, we further examined how AQP4 modulates osmotic effects on vasopressin (VP) neurons in rat supraoptic nucleus (SON) through interactions with GFAP in astrocytes. Methods : Brain slices from adult male rats were kept under osmotic stimulation. Western blot, co-immunoprecipitation, immunohistochem. and patch-clamp recordings were used for anal. of expressions and interactions between GFAP and AQP4, astrocyte-specific proteins in the SON, as well as their influence on VP neuronal activity. Data were analyzed using SPSS software. Results : Hyposmotic challenge (HOC) of acute SON slices caused an early (within 5 min) and transient increase in the colocalization of AQP4 with GFAP filaments. This effect was prominent at astrocytic processes surrounding VP neuron somata and was accompanied by inhibition of VP neuronal activity. Similar HOC effect was seen in the SON isolated from rats subjected to in vivo HOC, wherein a transiently increased mol. association between GFAP and AQP4 was detected using co-immunoprecipitation The late stage rebound excitation (10 min) of VP neurons in brain slices subjected to HOC and the associated astrocytic GFAP’s ‘return to normal’ were both hampered by 2-(nicotinamide)-1,3,4-thiadiazole, a specific AQP4 channel blocker that itself did not influence VP neuronal activity. Moreover, this agent prevented hyperosmotic stress-evoked excitation of VP neurons and associated reduction in GFAP filaments. Conclusion : These findings indicate that osmotically driven increase in VP neuronal activity requires the activation of AQP4, which determines a retraction of GFAP filaments.
Acta Physiologica published new progress about 51987-99-6. 51987-99-6 belongs to amides-buliding-blocks, auxiliary class Pyridine,Thiadiazole,Amine,Amide,Inhibitor, name is N-(1,3,4-Thiadiazol-2-yl)nicotinamide, and the molecular formula is C5H7F3O3, Name: N-(1,3,4-Thiadiazol-2-yl)nicotinamide.
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