Category: 27115-50-0

Yamamoto, Priscila A.; de Moraes, Natalia V.; Silva, Vinicius P.; Lepera, Jose S. published an article in 2016, the title of the article was Simultaneous analysis of biological indicators of exposure to ethylbenzene, styrene, toluene and xylene solvents in urine by HPLC-UV.Recommanded Product: 2-(4-Methylbenzamido)acetic acid And the article contains the following content:

A method for simultaneous anal. of phenylglyoxylic (PGA), mandelic (MA), hippuric (HA), ortho-, meta- and para-methylhippuric (o-, m- and p-MHA) acids in urine, by high performance liquid chromatog. with UV detection was developed and validated. The substances are biotransformation products used as biol. indicators to assess the exposure of workers, resp., to ethylbenzene, styrene, toluene and xylene. The urinary metabolites were separated using a C18 column and a mixture of 5 mM phosphate buffer pH 2.6:acetonitrile (92:8, volume/volume) as mobile phase. Urine samples were extracted with dichloromethane:ethyl acetate (70:30, volume/volume). The method used phenacetin as internal standard and was linear in the interval of 100-500 μg mL-1 for PGA and MA and 150-700 μg mL-1 for HA and MHA. The detection limits in μg mL-1, were 19.8 for PGA, 1.7 for MA, 4.1 for HA, 3.9 for o-MHA, 3.3 for m-MHA and 7.3 for p-MHA. Intra- and inter-assay precisions (as relative standard deviation) were all less than 15% and accuracy (as relative standard error) did not exceed 12.3%. The recovery was higher than 65% for all metabolites. The developed method will be applied to biol. evaluation of workers exposure to these solvents. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Recommanded Product: 2-(4-Methylbenzamido)acetic acid

The Article related to volatile organic compound exposure urine analysis hplc uv, Toxicology: Methods (Including Analysis) and other aspects.Recommanded Product: 2-(4-Methylbenzamido)acetic acid

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On July 15, 2013, Gagne, Sebastien published an article.Reference of 2-(4-Methylbenzamido)acetic acid The title of the article was Achieving greater selectivity for the analysis of o-, m-, p-methylhippuric acids in workers’ urine by ultra performance liquid chromatography coupled with tandem mass spectrometry. And the article contained the following:

A selective method analyzing sep. o-, m- and p-methylhippuric acid isomers in workers’ urine samples has been developed using ultra performance liquid chromatog. coupled with tandem mass spectrometry. Chromatog. separation has been optimized to resolve the three isomers at baseline. Combined with this optimal separation, the mass spectrometer allowed rapid switching from MRM scan to full scan and product ion scan within the chromatog. peak. This feature allowed the retention of analyte chem. structure information for the three methylhippuric acid isomers in parallel with the simultaneous acquisition of quant. data. Such an approach is unequaled for the reliability of the data generated and it can be applied to each isomer sep. The method was adjusted to a dynamic range between 0.2 mM and 8.12 mM for o-methylhippuric acid and p-methylhippuric acid, and between 0.41 mM and 16.23 mM for m-methylhippuric acid to cover the biol. exposure index. A negligible matrix effect was observed with the conditions used. Also, intra-day and inter-day precisions were both <6% for all the concentration levels tested and the accuracy was evaluated at 97%. The inclusion of simultaneous full scan acquisitions did not prevent the robustness of the quant. data. The method applied to the determination of inter-laboratory proficient test samples led to results in the tolerated range. Moreover, urine samples from workers were robustly quantified and the MHA levels were all below the biol. exposure index reference value. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid

The Article related to xylene exposure methylhippurate urine analysis worker, Toxicology: Methods (Including Analysis) and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid

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On October 31, 2012, Alwis, K. Udeni; Blount, Benjamin C.; Britt, April S.; Patel, Dhrusti; Ashley, David L. published an article.Category: amides-buliding-blocks The title of the article was Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS). And the article contained the following:

Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore, VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiol. half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatog. (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20 ng mL-1) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p < 0.0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Category: amides-buliding-blocks

The Article related to urine voc metabolite uplc ms tobacco smoke biomarker, Toxicology: Methods (Including Analysis) and other aspects.Category: amides-buliding-blocks

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On December 29, 2017, Lunn, Daniel B.; Yun, Young J.; Jorgenson, James W. published an article.Product Details of 27115-50-0 The title of the article was Retention and effective diffusion of model metabolites on porous graphitic carbon. And the article contained the following:

The study of metabolites in biol. samples is of high interest for a wide range of biol. and pharmaceutical applications. Reversed phase liquid chromatog. is a common technique used for the separation of metabolites, but it provides little retention for polar metabolites. An alternative to C18 bonded phases, porous graphitic carbon has the ability to provide significant retention for both non-polar and polar analytes. The goal of this work is to study the retention and effective diffusion properties of porous graphitic carbon, to see if it is suitable for the wide injection bands and long run times associated with long, packed capillary-scale separations The retention of a set of standard metabolites was studied for both stationary phases over a wide range of mobile phase conditions. Porous graphitic carbon benefits from significantly increased retention (often >100 fold) under initial gradient conditions for these metabolites, suggesting much improved ability to focus a wide injection band at the column inlet. The effective diffusion properties of these columns were studied using peak-parking experiments with the standard metabolites under a wide range of retention conditions. Under the high retention conditions, which can be associated with retention after injection loading for gradient separations, Deff/Dm ∼ 0.1 for both the C18-bonded and porous graphitic carbon columns. As C18 bonded particles are widely, and successfully used for long gradient separations without issue of increasing peak width from longitudinal diffusion, this suggests that porous graphitic carbon should be amenable for long runtime gradient separations as well. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Product Details of 27115-50-0

The Article related to metabolite retention diffusion porous graphitic carbon chromatog, effective diffusion, metabolites, porous graphitic carbon, sample pre-concentration, Biochemical Methods: Chromatographic and other aspects.Product Details of 27115-50-0

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Ghamari, Farhad; Bahrami, Abdulrahman; Yamini, Yadollah; Shahna, Farshid Ghorbani; Moghimbeigi, Abbas published an article in 2017, the title of the article was Hollow-fiber liquid-phase microextraction based on carrier-mediated transport for determination of urinary methyl hippuric acids.Reference of 2-(4-Methylbenzamido)acetic acid And the article contains the following content:

Hollow-fiber liquid-phase microextraction based on carrier-mediated transport for anal. of Me hippuric acids in aqueous samples is described. Under optimized conditions, relative recoveries of 83%-98% and limits of detection of 2-3μg L-1 were obtained, with linear calibration curves for the three isomers in the range of 10-50,000μg L-1. The method was applied for the determination of urine samples of volunteers at various working environments. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid

The Article related to urinary methyl hippuric acid microextraction, Biochemical Methods: Chromatographic and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid

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On March 20, 2013, Liu, Jie; Mei, Yong; Wang, Ya-nan; Gu, Ying-ying; Wang, Qian; Jiang, Jin-feng published an article.Category: amides-buliding-blocks The title of the article was Standardized study on determination of methylhippuric acids in urine by HPLC. And the article contained the following:

The aim of this paper is to get a better separation of methylhippuric acid isomers and establish a standardized method for the determination of methylhippuric acids in urine by HPLC. C18 column was used to determine the methylhippuric acid. The mobile phase was methanol-0.1% acetic acid (33:67). The detector was performed at λ=230 nm, at 0.7 mVmin. Calibration curves of three methylhippuric acids were linear in the range of 25μg/mL∼1000μg/mL, with a correlation coefficient more than 0.999. The average recovery was in the range of 91.36%∼101.36% and the average RSD was in the range of 2.0%∼9.6%. The min. detection concentrations of 2-MHA, 3-MHA, 4-MHA were resp. 0.47μg/mL, 0.46μg/mL, 0.41μg/mL. The separation of 4-methylhippuric acid and 3-methylhippuric acid was up to 1.44. The method is able to make 3-methylhippuric acid and 4-methylhippuric acid baseline separated, and the methodol. indicators are in line with the relevant requirements of the method for determination of chems. in the occupational health standard-setting Guide Part V. So it can be used to determine methylhippuric acids in urine of xylene exposure workers. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Category: amides-buliding-blocks

The Article related to hplc methylhippuric acid determination, Biochemical Methods: Chromatographic and other aspects.Category: amides-buliding-blocks

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Gika, Helen G.; Ji, Cheng; Theodoridis, Georgios A.; Michopoulos, Filippos; Kaplowitz, Neil; Wilson, Ian D. published an article in 2012, the title of the article was Investigation of chronic alcohol consumption in rodents via ultra-high-performance liquid chromatography-mass spectrometry based metabolite profiling.SDS of cas: 27115-50-0 And the article contains the following content:

Alc. consumption in man, when seen in its extreme form of alcoholism, is a complex and socially disruptive disorder that can result in significant levels of liver injury. Here the rodent “intragastric feeding model” was used together with UHPLC-TOFMS anal. to determine changes in global metabolite profiles for plasma and urine from alc. treated rats and mice compared to control animals. Multivariate statistical anal. (using principal components anal., PCA) revealed robust differences between profiles from control and alc.-treated animals from both species. A large number of metabolites were seen to differ between control and alc.-treated animals, for both biofluids. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).SDS of cas: 27115-50-0

The Article related to investigation chronic alc consumption rodents ultra high performance liquid, Toxicology: Ethanol and Alcoholism and other aspects.SDS of cas: 27115-50-0

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On May 31, 2022, Dehghan Haghighi, Javid; Hormozi, Maryam; Payandeh, Abolfazl published an article.Electric Literature of 27115-50-0 The title of the article was Blood serum levels of selected biomarkers of oxidative stress among printing workers occupationally exposed to low-levels of toluene and xylene. And the article contained the following:

Printing workers (PWs) are exposed to a mixture of solvents, yet the health risks associated with such exposuer are unknown. This study aimed to compare the serum levels of selected biomarkers of oxidative stress among occupationally exposed PWs to low-level of toluene and xylenes and unexposed controls. Associations between levels of such biomarkers and occupational exposures to toluene and xylene were also investigated. Urinary levels of hippuric acid (HA) and Me hippuric acids (MHAs) as exposure biomarkers of toluene and xylenes, resp., and serum levels of oxidative stress biomarkers, including total antioxidant capacity (TAC), malondialdehyde (MDA), and the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), were measured among the 84 subjects, comprising 44 PWs and 40 unexposed subjects. Mean concentrations of urinary HA and MHAs of PWs showed a significant increase compared with the unexposed controls. Although levels of urinary biomarkers of exposure to toluene (HA) and xylenes (MHAs) were well below the biol. exposure indexes (BEIs; ACGHI), PWs presented significantly increased serum levels of MDA, and significantly decreased serum activities of SOD and GPx compared to the unexposed controls. However, for serum TAC and CAT activity, no significant difference was observed between the two groups. Correlation analyses indicated that urinary levels of HA and MHAs were pos. correlated with MDA levels and neg. correlated with GPx and SOD. Our study suggested that the alterations evidenced in serum levels of MDA, SOD, and GPx could be involved in the oxidative stress caused by co-exposure to low levels of toluene and xylene. Further investigation is needed to clarify the effect of low-level occupational exposure to solvents among PWs. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Electric Literature of 27115-50-0

The Article related to toluene xylene blood serum printing worker biomarker oxidative stress, occupational exposure, antioxidants, oxidative stress, printing workers, toluene, xylene, Placeholder for records without volume info and other aspects.Electric Literature of 27115-50-0

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On April 20, 2022, Pal, Vineet Kumar; Li, Adela Jing; Zhu, Hongkai; Kannan, Kurunthachalam published an article.HPLC of Formula: 27115-50-0 The title of the article was Diurnal variability in urinary volatile organic compound metabolites and its association with oxidative stress biomarkers. And the article contained the following:

Volatile organic compounds (VOCs) are ubiquitous environmental pollutants that are associated with birth defects, leukemia, neurocognitive deficits, reproductive impairment and cancer in humans exposed to these compounds Exposure to VOCs can be assessed by measuring their metabolites in urine. Little is known, however, about the temporal variability in urinary VOC metabolite (VOCM) concentrations within- and between-individuals. In this study, we determined the variability in the concentrations of 38 VOCMs in urine samples collected from 19 healthy individuals across a period of 44 days. We also measured seven biomarkers of oxidative stress (lipid, protein and DNA damage) in urine to assess the relationship of VOC exposure to oxidative stress. Seventeen VOCMs had detection frequencies (DFs) of >60% in urine, and we limited further data anal. to those compounds The creatinine-adjusted geometric mean concentrations of VOCMs ranged from 2.70 渭g/g to 327 渭g/g in spot and 2.60 渭g/g to 551 渭g/g in first morning void (FMV) urine samples. Calculation of the intra-class correlation coefficients (ICCs) for 17 VOCM concentrations to assess their predictability and repeatability in urinary measurements showed ranges of 0.080-0.425 in spot and 0.050-0.749 in FMV urine samples, revealing notable within-individual variability. Our results suggest that taking only single measurements of VOCM concentrations in urine in epidemiol. investigations may lead to exposure misclassification. In addition, VOCM concentrations were significantly and pos. correlated with oxidative stress biomarkers. This study thus provides important information for formulating sampling strategies in the biomonitoring of VOC exposure in human populations. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).HPLC of Formula: 27115-50-0

The Article related to volatile organic compound metabolite oxidative stress biomarker, benzene, biomonitoring, intra-class correlation coefficients, oxidative stress, voc, variability, Placeholder for records without volume info and other aspects.HPLC of Formula: 27115-50-0

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On March 31, 2013, Barati, Behjat; Moghadam, Majid; Rahmati, Abbas; Mirkhani, Valiollah; Tangestaninejad, Shahram; Mohammadpoor-Baltork, Iraj published an article.Recommanded Product: 27115-50-0 The title of the article was Direct oxidation of alcohols to carboxylic acids over ruthenium hydride catalyst with diphenyl sulfoxide oxidant. And the article contained the following:

Direct oxidation of alcs. to their corresponding carboxylic acids with di-Ph sulfoxide oxidant over RuHCl(CO)(PPh3)3 catalyst was investigated. Mild reaction conditions, short reaction times and excellent yields make this method as an appealing way for preparation of carboxylic acids. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Recommanded Product: 27115-50-0

The Article related to alc diphenyl sulfoxide oxidation ruthenium catalyst, carboxylic acid preparation, General Organic Chemistry: Synthetic Methods and other aspects.Recommanded Product: 27115-50-0

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