Category: 186046-83-3

Fortunati, Simone published the artcileNovel amperometric genosensor based on peptide nucleic acid (PNA) probes immobilized on carbon nanotubes-screen printed electrodes for the determination of trace levels of non-amplified DNA in genetically modified (GM) soy, HPLC of Formula: 186046-83-3, the publication is Biosensors & Bioelectronics (2019), 7-14, database is CAplus and MEDLINE.

A novel amperometric genosensor based on PNA probes covalently bound on the surface of Single Walled Carbon Nanotubes – Screen Printed Electrodes (SWCNT-SPEs) was developed and validated in samples of non-amplified genomic DNA extracted from genetically modified (GM)-Soy. The sandwich assay is based on a first recognition of a 20-mer portion of the target DNA by a complementary PNA Capture Probe (CP) and a second hybridization with a PNA Signalling Probe (SP), with a complementary sequence to a different portion of the target DNA. The SP was labeled with biotin to measure current signal by means of a final incubation of an Alk. Phosphatase-streptavidin conjugate (ALP-Strp). The electrochem. detection was carried out using hydroquinone diphosphate (HQDP) as enzymic substrate. The genoassay provided a linear range from 250 pM to 2.5 nM, LOD of 64 pM and LOQ of 215 pM Excellent selectivity towards one base mismatch (1-MM) or scrambled (SCR) sequences was obtained. A simple protocol for extraction and anal. of non-amplified soybean genomic DNA without sample treatment was developed and validated. Our study provides insight into how the outstanding recognition efficiency of PNAs can be combined with the unique properties of CNTs in terms of signal response enhancement for direct detection of genomic DNA samples at the level of interest without previous amplification.

Biosensors & Bioelectronics published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, HPLC of Formula: 186046-83-3.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Grossmann, Tom N. published the artcileTarget-catalyzed transfer reactions for the amplified detection of RNA, Formula: C40H35N7O8, the publication is Angewandte Chemie, International Edition (2008), 47(37), 7119-7122, database is CAplus and MEDLINE.

Strong signals: The RNA-catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme-based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 500 attomol HIV RNA. The flexibility requirements of DNA- and RNA-catalyzed transfer reactions were tested.

Angewandte Chemie, International Edition published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Formula: C40H35N7O8.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Fischbach, Melanie published the artcileProtease Probes that Enable Excimer Signaling upon Scission, SDS of cas: 186046-83-3, the publication is Angewandte Chemie, International Edition (2014), 53(44), 11955-11959, database is CAplus and MEDLINE.

Peptide-based probes that fluoresce upon proteolytic cleavage are invaluable tools for monitoring protease activity. The read-out of protease activity through pyrene excimer signaling would be a valuable asset because the large Stokes shift and the long lifetime of the excimer emission facilitate measurements in autofluorescent media such as blood serum. However, proteolytic cleavage abolishes rather than installs the proximity relations required for excimer signaling. Herein, the authors introduce a new probe architecture to enable the switching on of pyrene excimer emission upon proteolytic scission. The method relies on hairpin-structured peptide nucleic acid (PNA)/peptide hybrids with pyrene units and anthraquinone-based quencher residues positioned in a zipper-like arrangement within the PNA stem. The excimer hairpin peptide beacons afforded up to a 50-fold enhancement of the pyrene excimer emission. Time-resolved measurements allowed the detection of matrix metalloprotease 7 in human blood serum.

Angewandte Chemie, International Edition published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, SDS of cas: 186046-83-3.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Nokihara, Kiyoshi published the artcileImproved coupling methods for the difficult amide-bond formation in solid-phase assembly, Formula: C40H35N7O8, the publication is Peptide Science (2013), 167-170, database is CAplus.

A symposium report. In the solid-phase peptide synthesis certain peptides are difficult to assemble because of steric hindrance and aggregation of mols., more over in such case removal of Nα-Fmoc group (Fmoc = 9-fluorenylmethoxycarbonyl) is also hindered. Yield and purity of desired peptides after cleavage are important for economical aspects, especially amide bond formation with costly unnatural amino acids as building blocks. The present report describes amide bond formation by elevated temperature to overcome poor coupling efficiency and to realize shorter coupling times. Addnl. racemization during Fmoc-removal at elevated temperature is also investigated.

Peptide Science published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Formula: C40H35N7O8.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Gasser, Gilles published the artcileSynthesis, characterisation and bioimaging of a fluorescent rhenium-containing PNA bioconjugate, COA of Formula: C40H35N7O8, the publication is Dalton Transactions (2012), 41(8), 2304-2313, database is CAplus and MEDLINE.

A new rhenium tricarbonyl complex of a bis(quinoline)-derived ligand (2-azido-N,N-bis((quinolin-2-yl)methyl)ethanamine, L-N₃), namely [Re(CO)₃(L-N₃)]Br was synthesized and characterized in-depth, including by x-ray crystallog. [Re(CO)₃(L-N₃)]Br exhibits a strong UV absorbance in the range 300-400 nm with a maximum at 322 nm, and upon photoexcitation, shows two distinct emission bands at about 430 and 560 nm in various solvents (water, ethylene glycol). [Re(CO)₃(L-N₃)]Br could be conjugated, on a solid phase, to a peptide nucleic acid (PNA) oligomer using the copper(I)-catalyzed azide-alkyne cycloaddition reaction (Cu-AAC, “click” chem.) and an alkyne-containing PNA building block to give Re-PNA. It was demonstrated that upon hybridization with a complementary DNA strand (DNA), the position of the maxima and emission intensity for the hybrid Re-PNA·DNA remained mainly unchanged compared to those of the single strand Re-PNA. The rhenium-containing PNA oligomer Re-PNA could be then mediated in living cells where they have been shown to be nontoxic contrary to the general notion that organometallic compounds are usually unstable under physiol. conditions and/or cytotoxic. Furthermore, Re-PNA could be detected in living cells using fluorescent microscopy.

Dalton Transactions published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, COA of Formula: C40H35N7O8.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Lin, Yiyang published the artcilePlasmonic Chirality Imprinting on Nucleobase-Displaying Supramolecular Nanohelices by Metal-Nucleobase Recognition, Recommanded Product: 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, the publication is Angewandte Chemie, International Edition (2017), 56(9), 2361-2365, database is CAplus and MEDLINE.

Supramol. self-assembly is an important process that enables the conception of complex structures mimicking biol. motifs. Herein, we constructed helical fibrils through chiral self-assembly of nucleobase-peptide conjugates (NPCs), where achiral nucleobases are helically displayed on the surface of fibrils, comparable to polymerized nucleic acids. Selective binding between DNA and the NPC fibrils was observed with fluorescence polarization. Taking advantage of metal-nucleobase recognition, we highlight the possibility of deposition/assembly of plasmonic nanoparticles onto the fibrillar constructs. In this approach, the supramol. chirality of NPCs can be adaptively imparted to metallic nanoparticles, covering them to generate structures with plasmonic chirality that exhibit significantly improved colloidal stability. The self-assembly of rationally designed NPCs into nanohelices is a promising way to engineer complex, optically diverse nucleobase-derived nanomaterials.

Angewandte Chemie, International Edition published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Recommanded Product: 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Browne, Elisse C. published the artcileEfficacy of peptide nucleic acid and selected conjugates against specific cellular pathologies of amyotrophic lateral sclerosis, Name: 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, the publication is Bioorganic & Medicinal Chemistry (2016), 24(7), 1520-1527, database is CAplus and MEDLINE.

Cellular studies have been undertaken on a nonamer peptide nucleic acid (PNA) sequence, which binds to mRNA encoding superoxide dismutase 1, and a series of peptide nucleic acids conjugated to synthetic lipophilic vitamin analogs including a recently prepared menadione (vitamin K) analog. Reduction of both mutant superoxide dismutase 1 inclusion formation and endoplasmic reticulum stress, two of the key cellular pathol. hallmarks in amyotrophic lateral sclerosis, by two of the prepared PNA oligomers is reported for the first time.

Bioorganic & Medicinal Chemistry published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Name: 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Kirin, Srecko I. published the artcileCellular uptake quantification of metalated peptide and peptide nucleic acid bioconjugates by atomic absorption spectroscopy, Quality Control of 186046-83-3, the publication is Angewandte Chemie, International Edition (2008), 47(5), 955-959, database is CAplus and MEDLINE.

At. absorption spectroscopy (AAS) of cobalt atoms is used as an accurate method to determine the cellular uptake and nuclear localization of metal bioconjugates. Surprisingly, the PNA conjugates show highest uptake efficiency, and an accumulation 150% higher than in the culture medium is achieved.

Angewandte Chemie, International Edition published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Quality Control of 186046-83-3.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Ogami, Keisuke published the artcileA pot-economical liquid-phase peptide nucleic acid synthesis enabled by a soluble tag-assisted method, Computed Properties of 186046-83-3, the publication is Chemistry Letters (2018), 47(2), 138-140, database is CAplus.

In a peptide nucleic acid (PNA), the base sequences are arranged onto a backbone of repeating units of N-(2-aminoethyl)glycine instead of on phosphodiester-linked riboses. PNA is a promising platform for the design of novel chem. probes because PNAs can form complementary base pairs with DNA and RNA that exhibit high enzymic stability. Described herein is a pot-economical liquid-phase PNA synthesis achieved using a soluble tag-assisted method. Intramol. trapping of the activated PNA monomer occurs much faster than the undesired intermol. “double hit”. Consequently, excess PNA monomers do not have to be rinsed away after each coupling, enabling one-pot coupling and deprotection without the need for precipitation

Chemistry Letters published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Computed Properties of 186046-83-3.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Ok, Tae-Dong published the artcileGNA/aegPNA Chimera Loaded with RNA Binding Preference, COA of Formula: C40H35N7O8, the publication is Chemistry – An Asian Journal (2011), 6(8), 1996-1999, database is CAplus and MEDLINE.

The authors have found a very simple strategy to improve binding preference of aegPNA [aeg = N-(2-aminoethyl)glycine] to RNA/DNA. Inspired by the structure of the GNA (glycol nucleic acid) monomer, a highly simple chiral PNA monomer was designed. By incorporating a few chiral acyclic γ-amino acid mols. into the achiral aegPNA backbone, the authors were able to make a chimeric PNA (chiPNA) with better RNA selectivity as well as antiparallel selectivity. The authors feel, to the best of their knowledge, this is the simplest monomeric unit that induces aegPNA to discriminate between RNA and DNA. Finally, the authors demonstrate that the chip-based competition-binding assay is an alternative tool to the quant. anal. of binding selectivities of modified PNAs. The authors believe that chiPNAs will expand the utility of PNAs and find diverse applications related to RNA targets in the future.

Chemistry – An Asian Journal published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, COA of Formula: C40H35N7O8.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics