On January 31, 2020, Doellinger, Joerg; Schneider, Andy; Hoeller, Marcell; Lasch, Peter published an article.Application of 79-07-2 The title of the article was Sample preparation by easy extraction and digestion (SPEED) – a universal, rapid, and detergent-free protocol for proteomics based on acid extraction. And the article contained the following:
The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a manner that enables efficient protein digestion for subsequent mass spectrometric anal. Today’s sample preparation strategies are commonly conceptualized around the removal of detergents, which are essential for extraction but strongly interfere with digestion and LC-MS. These multi-step preparations contribute to a lack of reproducibility as they are prone to losses, biases and contaminations, while being time-consuming and labor-intensive. We report a detergent-free method, named Sample Preparation by Easy Extraction and Digestion (SPEED), which consists of three mandatory steps, acidification, neutralization and digestion. SPEED is a universal method for peptide generation from various sources and is easily applicable even for lysis-resistant sample types as pure trifluoroacetic acid (TFA) is used for highly efficient protein extraction by complete sample dissolution The protocol is highly reproducible, virtually loss-less, enables very rapid sample processing and is superior to the detergent/chaotropic agent-based methods FASP, ISD-Urea and SP3 for quant. proteomics. SPEED holds the potential to dramatically simplify and standardize sample preparation while improving the depth of proteome coverage especially for challenging samples. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Application of 79-07-2
The Article related to escherichia staphylococcus hela proteomics acid extraction speed, tfa, automation, bacteria, detergent-free, digestion, label-free quantification, lysis, mass spectrometry, microbiome, pathogens, protein denaturation, proteomics, sample preparation and other aspects.Application of 79-07-2
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