《Evidence That Trimethyllysine Hydroxylase Catalyzes the Formation of (2S,3S)-3-Hydroxy-Nε-trimethyllysine》 was written by Reddy, Y. Vijayendar; Al Temimi, Abbas H. K.; White, Paul B.; Mecinovic, Jasmin. Reference of tert-Butyl N,N’-diisopropylcarbamimidateThis research focused ontrimethyllysine hydroxylase stereoselective hydroxylation 2S 3S hydroxy trimethyllysine. The article conveys some information:
Trimethyllysine hydroxylase (TMLH) is an Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase involved in the biomedically important carnitine biosynthesis pathway. A combination of synthetic and NMR studies provides direct evidence that human TMLH catalyzes the stereoselective conversion of (2S)-Nε-trimethyllysine to (2S,3S)-3-hydroxy-Nε-trimethyllysine. In the experiment, the researchers used many compounds, for example, tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8Reference of tert-Butyl N,N’-diisopropylcarbamimidate)
tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8) belongs to anime. Reaction with nitrous acid (HNO2), which functions as an acylating agent that is a source of the nitrosyl group (―NO), converts aliphatic primary amines to nitrogen and mixtures of alkenes and alcohols corresponding to the alkyl group in a complex process. This reaction has been used for analytical determination of primary amino groups in a procedure known as the Van Slyke method.Reference of tert-Butyl N,N’-diisopropylcarbamimidate
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics