《Bioorthogonal labelling of living bacteria using unnatural amino acids containing nitrones and a nitrone derivative of vancomycin》 was written by MacKenzie, Douglas A.; Sherratt, Allison R.; Chigrinova, Mariya; Kell, Arnold J.; Pezacki, John Paul. Quality Control of tert-Butyl N,N’-diisopropylcarbamimidateThis research focused onunnatural amino acid vancomycin derivative bacterial labeling. The article conveys some information:
Unnatural D-amino acids bearing endocyclic nitrones were developed for live-cell labeling of the bacterial peptidoglycan layer. Metabolic incorporation of D-Lys and D-Ala derivatives bearing different endocyclic nitrones was observed in E. coli, L. innocua, and L. lactis. The incorporated nitrones of these bacteria then rapidly underwent strain-promoted alkyne-nitrone cycloaddition (SPANC) reactions affording chem. modified bacteria. In the experimental materials used by the author, we found tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8Quality Control of tert-Butyl N,N’-diisopropylcarbamimidate)
tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8) belongs to anime. Left-handed and right-handed forms (mirror-image configurations, known as optical isomers or enantiomers) are possible when all the substituents on the central nitrogen atom are different (i.e., the nitrogen is chiral). With amines, there is extremely rapid inversion in which the two configurations are interconverted.Quality Control of tert-Butyl N,N’-diisopropylcarbamimidate
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics