Distribution and degradation of [3H]-methotrexate after intravenous and cerebral intraventricular injection in primates was written by Kimelberg, Harold K.;Biddlecome, Sandra M.;Bourke, Robert S.. And the article was included in Cancer Research in 1977.Electric Literature of C20H20N8Na2O5 This article mentions the following:
Four h after either a single injection or continuous infusion of Na methotrexate (Na I) [7413-34-5] plus purified [3′,5′,9(n3-3H]I in cynomolgus or rhesus monkeys, 80 to 98% of the 3H radioactivity present in the plasma was found not to represent intact I. The percentage of 3H-containing I products in the urine after 4 h was considerably less, although more variable. This variability seemed to be related variability in the amount of the total dose excreted. Non-I products were also found in selected tissues and the percentage of intact I found 4 h after i.v. injection varied from 2 to 26%. The percentage of intact I was routinely measured by comparing the values obtained using the dihydrofolate reductase assay with values based on the specific activity of [3′,5′,9(n)-3H]I. Results obtained by diethylaminoethyl column chromatog. on a few samples, however, showed good agreement with results from the reductase assay. [3′,5′,9(N)-3H]I products appeared in peaks eluting from the diethylaminoethyl column both earlier and later than the I peak, with the earlier peaks being present in only small amounts in the urine. After continuous i.v. infusion, only 2% or less of the radioactivity found in the cerebrospinal fluid after 4 h represented intact I, with the remaining radioactivity eluting much earlier than I. In contrast, after direct injection into the left lateral ventricle, all the 3H radioactivity in both cerebrospinal fluid and brain tissue represented intact I for up to 4 h after injection. The appearance of I products in the plasma and selected tissues of these primates a short time after i.v. injection is compared to other work in exptl. animals and man and suggests a greater metabolism of I than was previously suspected. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Electric Literature of C20H20N8Na2O5).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Electric Literature of C20H20N8Na2O5
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics