Purineless death as a link between growth rate and cytotoxicity by methotrexate was written by Hryniuk, William M.. And the article was included in Cancer Research in 1972.Product Details of 7413-34-5 This article mentions the following:
In murine S-phase lymphoblasts, Na methotrexate (Na I) [7413-34-5] produces a purineless state, the degree of which depends upon the growth rate of the population. The purineless state probably contributes to the lethal effect of I, and may explain in part the relation between growth rate and cytotoxicity of antifolates. I decreased cloning efficiency and inhibited DNA, RNA, and protein synthesis in L5178Y murine lymphoblasts from cultures proliferating at different rates. The effects of I increased with the proliferative rate. Hypoxanthine [68-94-0] completely prevented the biochem. effects of I and partially protected the cells against lethal effects of I. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Product Details of 7413-34-5).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. Compared to amines, amides are very weak bases and do not have clearly defined acid–base properties in water. On the other hand, amides are much stronger bases than esters, aldehydes, and ketones. In simple aromatic amides, fragmentation occurs on both sides of the carbonyl group. If a hydrogen is available in N-substituted aromatic amides, it tends to migrate and form an aromatic amine and the loss of a ketene.Product Details of 7413-34-5
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics