Distribution of methotrexate in rat tissues was written by Newbold, P. C. H.. And the article was included in British Journal of Dermatology in 1974.Quality Control of Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate This article mentions the following:
Following i.p. injection of tritiated methotrexate di-Na salt (I di-Na salt) [7413-34-5], the amounts of I taken up by the skin and liver of rats were doubled by exptl. folic acid [59-30-3] depletion. In contrast, folate deficiency did not significantly increase I uptake by the kidneys or intestines. Skin uptake of I was increased about 6-fold by prior stripping (psoriasis model); hepatic uptake was still marked in this model. I taken up by the tissues was partially displaced by pteridines with an infinity for dehydrofolate reductase. Displacement was most efficient with I itself, but also significant with both dihydrofolate [4033-27-6] and folic acid and measurable with leucovorin [58-05-9]; 5-methyl folate [53464-60-1] had virtually no effect. Thus, a risk of hepatic injury is probably inseparable from the therapeutic action of I in psoriasis. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Quality Control of Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Quality Control of Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics