NAD+ -boosting molecules suppress mast cell degranulation and anaphylactic responses in mice was written by Kim, Hyun-Woo;Ryoo, Ga-Hee;Jang, Hyun-Young;Rah, So-Young;Lee, Dong Hyun;Kim, Do-Kyun;Bae, Eun Ju;Park, Byung-Hyun. And the article was included in Theranostics in 2022.Synthetic Route of C11H15N2O8P The following contents are mentioned in the article:
NAD (NAD +) acts as a cofactor for multiple biol. processes. While previous research has revealed that the NAD + declines associated with aging contributes to an impairment of immune cells, its role in mast cell function, especially in response to an anaphylactic condition, has remained unexplored. Author tested whether the restoration of cellular NAD + concentration by the supplementation of NAD + boosting mols. prevented mast cell degranulation and anaphylactic responses. Bone marrow derived mast cells (BMMCs) and human cord blood derived mast cells were treated with NAD + precursors NMN (NMN) and nicotinamide riboside (NR), and FceRI downstream signaling was assessed. Animal models of passive systemic anaphylaxis (PSA) and passive cutaneous anaphylaxis (PCA) were used to investigate the effects of NAD + precursors in the anaphylactic responses of mice. Treatment of murine BMMCs and human cord blood derived mast cells with NAD + precursors repressed intracellular signaling downstream of FceRI, as well as the release of inflammatory cytokines and lipid mediators. The i.p. administration of NMN or NR also markedly attenuated IgE-mediated anaphylactic responses in mouse models of PSA and PCA. These beneficial effects of NAD + precursors, however, were attenuated in mast cell-specific Sirt6 knockout mice, indicating a Sirt6 dependency for their action. NAD + precursors may serve as an effective therapeutic strategy that limits mast cell-mediated anaphylactic responses. This study involved multiple reactions and reactants, such as ((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7Synthetic Route of C11H15N2O8P).
((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7) belongs to amides. The solubilities of amides and esters are roughly comparable. Typically amides are less soluble than comparable amines and carboxylic acids since these compounds can both donate and accept hydrogen bonds. Tertiary amides, with the important exception of N,N-dimethylformamide, exhibit low solubility in water. Amides are not in general accessible by the direct condensation of amines with carboxylic acids for two reasons: first, both components are readily deactivated by a transfer of a proton from the acid to the amine and second, the hydroxy unit on the carbonyl of the acid is a relatively poor leaving group. Nevertheless, the formation of five- and six-membered rings is often surprisingly simple provided that other factors can be brought into play to assist in the condensation.Synthetic Route of C11H15N2O8P
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics