Hu, Yang-mei-qing published the artcileThe preparation of polyclonal antibody against chlordimeform and establishment of detection by indirect competitive ELISA, Product Details of C2H5NO, the main research area is polyclonal antibody chlordimeform indirect competitive ELISA; Chlordimeform; IC-ELISA; artificial antigen; polyclonal antibody.
Chlordimeform is a chem. pesticide that is highly carcinogenic and toxic. The purpose of this study was to establish an ELISA (ELISA) method for the detection of chlordimeform in aquaculture and fish farming. Chlordimeform was coupled with bovine serum albumin (BSA) and ovalbumin (OVA) as carrier proteins. A chlordimeform-BSA conjugate was used as an immunogen, and chlordimeform-OVA was used as a coating antigen. Chlordimeform-BSA was used to immunize rabbits, and a polyclonal antibody was prepared An indirect competitive ELISA (IC-ELISA) was established to detect chlordimeform. The working range of the established IC-ELISA method for chlordimeform detection was 1-20 ng/mL. The IC50 was 3.126 ng/mL, and the lower limit of detection (LOD) of chlordimeform was 0.637 ng/mL. The recovery of chlordimeform from spiked water samples ranged from 81% to 107%. An anti-chlordimeform polyclonal antibody was successfully developed, and a novel IC-ELISA was established to detect chlordimeform in aquaculture.
Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes published new progress about Antiserums. 123-39-7 belongs to class amides-buliding-blocks, name is N-Methylformamide, and the molecular formula is C2H5NO, Product Details of C2H5NO.
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics