Kaiser, Emil Thomas; Lo, Kwok-Wing published an article in 1969, the title of the article was Carbonic anhydrase catalyzed hydrolysis of 2-hydroxy-5-nitro-α-toluenesulfonic acid sultone.Application of 97-09-6 And the article contains the following content:
An ionizable group in bovine carbonic anhydrase (BCA) with a pK of 7.3 appears to be involved in the enzyme-catalyzed hydrolysis of 2-hydroxy-5-nitro-α-toluenesulfonic acid sultone (I). Similar observations have been reported previously for the pH-rate behavior of the BCA-catalyzed hydration of CO2, hydration of carbonyl compounds, and hydrolysis of nitrophenyl esters of carboxylic acids. The BCA-catalyzed hydrolysis of I is subject to sulfonamide inhibition as are the other reactions mentioned above. Also, human carbonic anhydrases B and C have been demonstrated to be effective catalysts for the hydrolysis of I. On the basis of observations taken in conjunction with those of other investigations, it is proposed that a Zn-bound hydroxide ion is the active catalytic species in carbonic anhydrase action, and a cyclic mechanism for the carbonic anhydrase catalyzed solvolysis of I, involving no net proton transfer to the solvent is suggested. The experimental process involved the reaction of 3-Nitro-4-chlorobenzenesulfonamide(cas: 97-09-6).Application of 97-09-6
The Article related to carbonic anhydrase, bovine carbonic anhydrase, catalytic species carbonic anhydrase, reaction kinetics and other aspects.Application of 97-09-6
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