Month: January 2023

Pharmacological research on new salicylic and gentisic acid derivatives. I. Toxicity was written by Preziosi, P.. And the article was included in Archivio Italiano di Scienze Farmacologiche in 1955.Category: amides-buliding-blocks This article mentions the following:

The L.D.₅₀ of N-diethylsalicylamide (I), o-ethoxybenzamide (II), o-diethylaminoethoxybenzamide (III), salicoyl hydrazide (IV), N-acetylsalicoyl hydrazide (V), gentisamide (VI) and diacetylgentisic acid (VII) on rats, subcutaneously, was 121, 111, 70, 10, 181, 101, and 148%, resp., that of salicylamide (VIII); and on rabbits, intravenously, 92, 106, 107, 116, 145, 212, and 139%, resp. In experiments of chronic toxicity, with doses 1.51-3.02 times the therapeutic ones, the above behavior was confirmed: I, V, VI and VII gave no unfavorable effect, VIII was toxic in 50-100 mg. doses, II inhibited rat growth. None of the above drugs affected the blood crasis. On yeast respiration, the most toxic drug was IV, followed by I and V. The influence of the different groups (carboxyl, hydrazine, acetyl) on the drug toxicity is stressed. In the experiment, the researchers used many compounds, for example, N,N-Diethylsalicylamide (cas: 19311-91-2Category: amides-buliding-blocks).

N,N-Diethylsalicylamide (cas: 19311-91-2) belongs to amides. Amides include many other important biological compounds, as well as many drugs like paracetamol, penicillin and LSD. Low-molecular-weight amides, such as dimethylformamide, are common solvents. The presence of the amide group –C(=O)N– is generally easily established, at least in small molecules. It can be distinguished from nitro and cyano groups in IR spectra. Amides exhibit a moderately intense νCO band near 1650 cm−1. By 1H NMR spectroscopy, CONHR signals occur at low fields. In X-ray crystallography, the C(=O)N center together with the three immediately adjacent atoms characteristically define a plane.Category: amides-buliding-blocks

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

The combined effect of tranilast 8% liposomal gel on the final cosmesis of acne scarring in patients concomitantly treated by isotretinoin: prospective, double-blind, split-face study. was written by Weinstein, A;Koren, A;Sprecher, E;Zur, E;Mehrabi, J N;Artzi, O. And the article was included in Clinical and experimental dermatology in 2020.Electric Literature of C18H17NO5 This article mentions the following:

BACKGROUND: Tranilast [N-(3,4-dimethoxycinnamoyl) anthranilic acid] has never been investigated for the prevention and treatment of acne scars. AIM: To evaluate the efficacy and safety of tranilast 8% gel in improving the final appearance of patients with acne scarring concomitantly treated by isotretinoin. METHODS: This was a prospective, double-blind, split-face study, which enrolled 40 otherwise healthy participants (aged 18-49 years) with facial acne scars. For each patient, one half of the face were treated with tranilast 8% liposomal gel and the other half with a water-based placebo. Using the Global Aesthetic Improvement Scale (GAIS), acne scars were evaluated by two dermatologists and by the patients, and the patients also rated their satisfaction with the treatment and reported adverse effects. RESULTS: In total, 32 participants completed the trial. The mean GAIS scores at 5 months post-treatment were significantly lower (better outcome) for the tranilast-treated side than the placebo-treated areas in patients concomitantly treated with isotretinoin (P < 0.001). All the isotretinoin-treated patients reported greater satisfaction and better general improvement in the skin’s appearance and texture, and also greater improvement of pigment and redness on the tranilast 8% gel-treated side compared with the control side. CONCLUSION: Combined topical application of tranilast 8% gel twice daily with oral isotretinoin treatment in the active phase of acne vulgaris may result in fewer scars, finer skin texture and enhanced appearance. In the experiment, the researchers used many compounds, for example, 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8Electric Literature of C18H17NO5).

2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8) belongs to amides. The solubilities of amides and esters are roughly comparable. Typically amides are less soluble than comparable amines and carboxylic acids since these compounds can both donate and accept hydrogen bonds. Tertiary amides, with the important exception of N,N-dimethylformamide, exhibit low solubility in water. Amides can be recrystallised from large quantities of water, ethanol, ethanol/ether, aqueous ethanol, chloroform/toluene, chloroform or acetic acid. The likely impurities are the parent acids or the alkyl esters from which they have been made. The former can be removed by thorough washing with aqueous ammonia followed by recrystallisation, whereas elimination of the latter is by trituration or recrystallisation from an organic solvent.Electric Literature of C18H17NO5

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Design, Synthesis, Molecular Modeling and Anti-Hyperglycemic Evaluation of Quinazoline-Sulfonylurea Hybrids as Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) and Sulfonylurea Receptor (SUR) Agonists was written by El-Zahabi, Mohamed Ayman;Bamanie, Faida H.;Ghareeb, Salah;Alshaeri, Heba K.;Alasmari, Moudi M.;Moustafa, Mohamed;Al-Marzooki, Zohair;Zayed, Mohamed F.. And the article was included in International Journal of Molecular Sciences in 2022.Application In Synthesis of 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide This article mentions the following:

New quinazoline-sulfonylurea hybrids were prepared and examined for their in vivo anti-hyperglycemic activities in STZ-induced hyperglycemic rats using glibenclamide as a reference drug. Compounds VI-6-a, V, IV-4, VI-4-c, IV-6, VI-2-a, IV-1, and IV-2 were more potent than the reference glibenclamide. They induced significant reduction in the blood glucose levels of diabetic rats: 78.2, 73.9, 71.4, 67.3, 62, 60.7, 58.4, and 55.9%, resp., while the reference glibenclamide had 55.4%. Compounds IV-1, VI-2-a, IV-2, V, and IV-6 showed more prolonged antidiabetic activity than glibenclamide. Moreover, mol. docking and pharmacokinetic studies were performed to examine binding modes of the prepared compounds against peroxisome proliferator-activated receptor gamma (PPARγ). The highest active compounds exhibited good binding affinity with high free energy of binding against PPARγ. In silico absorption, distribution, metabolism, elimination and toxicity (ADMET) studies were performed to investigate pharmacokinetics and safety of the synthesized compounds They showed considerable human intestinal absorption with low toxicity profile. In the experiment, the researchers used many compounds, for example, 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8Application In Synthesis of 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide).

5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. In simple aromatic amides, fragmentation occurs on both sides of the carbonyl group. If a hydrogen is available in N-substituted aromatic amides, it tends to migrate and form an aromatic amine and the loss of a ketene.Application In Synthesis of 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Pharmacological and therapeutic properties of carrier bound methotrexate against tumor confined to a third space body compartment was written by Chu, Barbara C. F.;Howell, Stephen B.. And the article was included in Journal of Pharmacology and Experimental Therapeutics in 1981.Recommanded Product: 7413-34-5 This article mentions the following:

The pharmacokinetics and therapeutic effectiveness of Na methotrexate (MTX)(I Na salt) [7413-34-5] and MTX covalently bound to bovine serum albumin (MTX-BSA) and poly-l-lysine, MW 3,000 (MTX-PLL 3K) or MW 40,000 to 60,000 (MTX-PLL 40-60K) were compared when these drugs were injected directly into the pleural cavities of BDF, mice containing the L1210 tumor. Simultaneous measurements of drug levels in both pleural fluid and blood after a single dose demonstrated that free MTX and MTX-PLL 3K were cleared from the pleural cavity and blood within 4 h, MTX-PLL 40K-60K was cleared within 2 h, and MTX-BSA was still present in the tumor compartment at 48 h. The coupling of MTX to these carriers increased its toxicity by extending the half-life of MTX-BSA within the animal and by incorporating a toxic PLL derivative as a carrier. At equitoxic doses, a single dose of MTX-BSA gave a peak increase in lifespan (ILS) of 50% (at 35 mg/kg) compared with a peak ILS of 30 to 35% for both free drug (at 95 mg/kg) and the MTX-PLL derivatives (at 1.4-6 mg/kg). Systemic administration of sufficient Ca leucovorin [1492-18-8] to provide partial marrow protection compromised the antitumor activity of both MTX and MTX-BSA in the pleural cavity, and although leucovorin permitted higher doses to be used, this resulted in only a small increase in peak ILS for MTX-BSA on a single dose schedule. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Recommanded Product: 7413-34-5).

Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Recommanded Product: 7413-34-5

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Introduction of Z-GP scaffold into procarbazine reduces spermatoxicity and myelosuppression was written by Wang, Rikang;Zhang, Chao;Zheng, Chaojun;Li, Huilan;Xie, Xinshu;Jin, Yi;Liu, Zhijun;Chen, Heru. And the article was included in Bioorganic Chemistry in 2019.Synthetic Route of C11H13NO2 This article mentions the following:

Incorporation of carbobenzoxy-glycylprolyl (Z-GP) to either α or β position of the hydrazine moiety in procarbazine (Pcb) has been carried on in 5-steps process. The overall yield was 32.7%. The new entity Z-GP-Pcb was confirmed targeting to fibroblast activation protein-α (FAPα). Z-GP-Pcb may be hydrolyzed by either isolated rhFAPα or tumor homogenate. It was shown far less cytotoxicity against NCI-H460 cell line than Pcb. Z-GP-Pcb was displayed the potency to reduce spermatoxcity in H22-bearing mice. The mechanism may be ascribed to the blockade of dehydrogenation by α-glycerolphosphate dehydrogenase. This candidate was further proved equal antitumor activity to Pcb. However, the introduction of Z-GP scaffold decreased myelosuppression. All the evidences support that Z-GP-Pcb is a better antitumor agent than Pcb. In the experiment, the researchers used many compounds, for example, 4-Formyl-N-isopropylbenzamide (cas: 13255-50-0Synthetic Route of C11H13NO2).

4-Formyl-N-isopropylbenzamide (cas: 13255-50-0) belongs to amides. Because of the greater electronegativity of oxygen, the carbonyl (C=O) is a stronger dipole than the N–C dipole. The presence of a C=O dipole and, to a lesser extent a N–C dipole, allows amides to act as H-bond acceptors. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Synthetic Route of C11H13NO2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

The design and development of potent small molecules as anticancer agents targeting EGFR TK and tubulin polymerization was written by Ihmaid, Saleh;Ahmed, Hany E. A.;Zayed, Mohamed F.. And the article was included in International Journal of Molecular Sciences in 2018.Computed Properties of C18H17NO5 This article mentions the following:

Some novel anthranilate diamides derivatives 4a-e, 6a-c and 9a-d were designed and synthesized to be evaluated for their in vitro anticancer activity. Structures of all newly synthesized compounds were confirmed by infra-red (IR), high-resolution mass (HR-MS) spectra, ¹H NMR (NMR) and ¹³C NMR (NMR) analyses. Cytotoxic screening was performed according to (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) assay method using erlotinib as a reference drug against two different types of breast cancer cells. The mol. docking study was performed for representative compounds against two targets, epidermal growth factor receptor (EGFR) and tubulin in colchicine binding site to assess their binding affinities in order to rationalize their anticancer activity in a qual. way. The data obtained from the mol. modeling was correlated with that obtained from the biol. screening. These data showed considerable anticancer activity for these newly synthesized compounds Biol. data for most of the anthranilate diamide showed excellent activity with nanomolar or sub nanomolar half maximal inhibitory concentration (IC₅₀) values against tumor cells. EGFR tyrosine kinase (TK) inhibition assay, tubulin inhibition assay and apoptosis anal. were performed for selected compounds to get more details about their mechanism of action. Extensive structure activity relationship (SAR) analyses were also carried out. In the experiment, the researchers used many compounds, for example, 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8Computed Properties of C18H17NO5).

2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8) belongs to amides. Compared to amines, amides are very weak bases and do not have clearly defined acid–base properties in water. On the other hand, amides are much stronger bases than esters, aldehydes, and ketones. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Computed Properties of C18H17NO5

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Experimental and modeling investigation of Glibenclamide solubility in supercritical carbon dioxide was written by Esfandiari, Nadia;Sajadian, Seyed Ali. And the article was included in Fluid Phase Equilibria in 2022.Product Details of 10238-21-8 This article mentions the following:

Optimized production of micro and nanoparticles using the supercritical fluid technique requires information on the solubility of pharmaceutical materials in the supercritical fluid. In this article, Glibenclamide solubility in the SC-CO₂ was investigated for the first time at the temperature range 0f 308-338 K and pressure range of 12-30 MPa. Under this condition, the mole fraction of Glibenclamide was determined in the range 0.8 x 10^-6 to 8.03 x 10^-5. Furthermore, the six empirical models (i.e. Chrastil, Bartle et al., Mendez-Santiago and Teja (MST), Sparks et al., Bian et al., Sodeifian et al.) and PR-EoS with vdW2 mixing rule were utilized to correlate the solubility The results confirmed that Sodeifian et al. and MST models exhibited the highest accuracy with resp. R_adj of 0.996 and 0.9859. Finally, the total (41.65 kJ mol^-1), vaporization (65.5 kJ mol^-1), and solvation. (-23.85 kJ mol^-1) enthalpies were estimated by Chrastil’s model and Bartle et al. In the experiment, the researchers used many compounds, for example, 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8Product Details of 10238-21-8).

5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. The presence of the amide group –C(=O)N– is generally easily established, at least in small molecules. It can be distinguished from nitro and cyano groups in IR spectra. Amides exhibit a moderately intense νCO band near 1650 cm−1. By 1H NMR spectroscopy, CONHR signals occur at low fields. In X-ray crystallography, the C(=O)N center together with the three immediately adjacent atoms characteristically define a plane.Product Details of 10238-21-8

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Bioaccumulation and bioamplification of pharmaceuticals and endocrine disruptors in aquatic insects was written by Veseli, Marina;Rozman, Marko;Vilenica, Marina;Petrovic, Mira;Previsic, Ana. And the article was included in Science of the Total Environment in 2022.Category: amides-buliding-blocks This article mentions the following:

Environmental fate of emerging contaminants such as pharmaceuticals and endocrine disrupting compounds at the aquatic terrestrial boundary are largely unexplored. Aquatic insects connect aquatic and terrestrial food webs as their life cycle includes aquatic and terrestrial life stages, thus they represent an important inter-habitat linkage not only for energy and nutrient flow, but also for contaminant transfer to terrestrial environments. We measured the concentrations of pharmaceuticals and endocrine disrupting compounds in the larval and adult tissues (last larval stages and teneral adults) of five Odonata species sampled in a wastewater-impacted river, in order to examine their bioaccumulation and bioamplification at different taxonomic levels. Twenty different compounds were bioaccumulated in insect tissues, with majority having higher concentrations (up to 90% higher) in aquatic larvae compared to terrestrial adults (reaching 88 ng/g for 1H-benzotriazole). However, increased concentration in adults was observed for seven compounds in at least one suborder (41% of the accumulated), confirming contaminants bioamplification across the metamorphosis. Both, bioaccumulation and bioamplification differed at various taxa levels; the order (Odonata), suborder (Anisoptera and Zygoptera) and species level. Highest variability was observed between Anisoptera and Zygoptera, due to the underlying differences in their ecol. Generally, Zygoptera had higher concentrations of contaminants in both larvae and adults. Addnl., we aimed at predicting effects of contaminant properties on bioaccumulation and bioamplification patterns using the commonly used physicochem. and pharmacokinetic descriptors on both order and suborder levels, however, neither of the two processes could be consistently predicted with simple linear models. Our study highlights the importance of taxonomy in studies aiming at advancing the understanding of contaminant exchange between aquatic and terrestrial food webs, as higher taxonomic categories include ecol. diverse groups, whose contribution to “the dark side of subsidies” could substantially differ. In the experiment, the researchers used many compounds, for example, 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8Category: amides-buliding-blocks).

5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8) belongs to amides. Compared to amines, amides are very weak bases and do not have clearly defined acid–base properties in water. On the other hand, amides are much stronger bases than esters, aldehydes, and ketones. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.Category: amides-buliding-blocks

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Synthesis and biological evaluation of N-(cyclopropyl)benzamide-benzophenone hybrids as novel and selective p38 mitogen activated protein kinase (MAPK) inhibitors was written by Heo, Jinyuk;Shin, Hanbo;Lee, Jun;Kim, Taelim;Inn, Kyung-Soo;Kim, Nam-Jung. And the article was included in Bioorganic & Medicinal Chemistry Letters in 2015.Recommanded Product: 4-Bromo-N-methoxy-N-methylbenzamide This article mentions the following:

A series of hybrid mols. consisting of benzophenone derivatives and N-cyclopropyl-3-methylbenzamide derivatives were synthesized and biol. evaluated as novel p38 mitogen activated protein kinase (MAPK) inhibitors. In particular, the authors found that one compound displayed potent p38α MAPK inhibitory activity (IC₅₀ = 0.027 μM), high kinase selectivity, and significant antiinflammatory activity in THP-1 monocyte cells [i.e., human monocytic leukemia cell line]. The synthesis of the target compounds was achieved by a reaction of N-cyclopropyl-4-methyl-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzamide with [(hydroxyethoxy)phenyl](bromophenyl)methanone derivatives and analogs. In the experiment, the researchers used many compounds, for example, 4-Bromo-N-methoxy-N-methylbenzamide (cas: 192436-83-2Recommanded Product: 4-Bromo-N-methoxy-N-methylbenzamide).

4-Bromo-N-methoxy-N-methylbenzamide (cas: 192436-83-2) belongs to amides. The amide group is called a peptide bond when it is part of the main chain of a protein, and an isopeptide bond when it occurs in a side chain, such as in the amino acids asparagine and glutamine. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.Recommanded Product: 4-Bromo-N-methoxy-N-methylbenzamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Age-related differences in transient receptor potential vanilloid 1 and 2 expression patterns in the trigeminal ganglion neurons contribute to changes in the palatal mucosal heat pain sensitivity was written by Oto, Tatsuki;Urata, Kentaro;Hayashi, Yoshinori;Hitomi, Suzuro;Shibuta, Ikuko;Iwata, Koichi;Iinuma, Toshimitsu;Shinoda, Masamichi. And the article was included in Tohoku Journal of Experimental Medicine in 2022.Safety of 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid This article mentions the following:

Aging affects various sensory functions of the body. However, the effect on the oral mucosal nociception has remain unclear, so this elucidation is very important. Therefore, this study aimed to evaluate the effect of age-related changes in transient receptor potential vanilloid 1 (TRPV1) and TRPV2 expression in the trigeminal ganglion (TG) neurons on intraoral mucosal heat sensitivity in the senescence-accelerated mouse prone 8 (SAMP8) model. We used 23-wk-old (aged) and 7-wk-old (young) SAMP8 mice. Heat stimulation was applied to the palatal mucosa under light anesthesia; moreover, the heat head withdrawal threshold (HHWT) was measured. We counted the number of TRPV1-immunoreactive (IR) and TRPV2-IR TG neurons innervating the palatal mucosa. Addnl., we investigated changes in HHWT when TRPV1 or TRPV2 antagonists (SB366791 or Tranilast) were administered to the palatal mucosa. Aged SAMP8 mice showed a higher HHWT than young SAMP8 mice. Compared with the aged SAMP8 mice, young SAMP8 mice showed a larger number of TRPV1-IR small-diameter neurons and a smaller number of TRPV2-IR medium-sized neurons innervating the palatal mucosa. SB366791 administration increased the HHWT in young, but not aged SAMP8 mice. Contrastingly, Tranilast administration increased the HHWT in aged, but not young SAMP8 mice. These results suggest that the modulation of heat pain sensitivity in the oral mucosa due to aging is dependent on changes in the TRPV1 and TRPV2 expression patterns in the TG neurons innervating the palatal mucosa. In the experiment, the researchers used many compounds, for example, 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8Safety of 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid).

2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8) belongs to amides. The solubilities of amides and esters are roughly comparable. Typically amides are less soluble than comparable amines and carboxylic acids since these compounds can both donate and accept hydrogen bonds. Tertiary amides, with the important exception of N,N-dimethylformamide, exhibit low solubility in water. The presence of the amide group –C(=O)N– is generally easily established, at least in small molecules. It can be distinguished from nitro and cyano groups in IR spectra. Amides exhibit a moderately intense νCO band near 1650 cm−1. By 1H NMR spectroscopy, CONHR signals occur at low fields. In X-ray crystallography, the C(=O)N center together with the three immediately adjacent atoms characteristically define a plane.Safety of 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics