Month: January 2023

The overexpression of the SAPB of Bacillus pumilus CBS and mutated sapB-L31I/T33S/N99Y alkaline proteases in Bacillus subtilis DB430: New attractive properties for the mutant enzyme was written by Zarai Jaouadi, Nadia;Jaouadi, Bassem;Aghajari, Nushin;Bejar, Samir. And the article was included in Bioresource Technology in 2012.Related Products of 10543-57-4 This article mentions the following:

The sapB gene encoding for Bacillus pumilus CBS protease (SAPB) and the triple mutated sapB-L31I/T33S/N99Y gene were cloned and overexpressed in the protease-deficient Bacillus subtilis DB430 using an Escherichia coli-Bacillus shuttle vector pBSMuL2. The 34,625.13 and 34,675.11-Da enzymes were purified from the culture supernatant of B. subtilis expressing the wild-type and mutated genes, resp. The purified proteases showed the same N-terminal sequences and biochem. properties of those expressed in E. coli. Further investigations demonstrated that, compared to wild-type and other proteases, SAPB-L31I/T33S/N99Y had the highest catalytic efficiency and the best degree of hydrolysis. The mutant enzyme was also noted to exhibit a number of newly explored properties that are highly valued in the marketplace, namely considerable stability to detergents, higher resistance towards organic solvents, and potent dehairing ability. Overall, the findings indicated that SAPB-L31I/T33S/N99Y is a promising candidate for future use in a wide range of industrial and com. applications. In the experiment, the researchers used many compounds, for example, N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4Related Products of 10543-57-4).

N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Related Products of 10543-57-4

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Functional Amphiphilic Poly(2-oxazoline) Block Copolymers as Drug Carriers: the Relationship between Structure and Drug Loading Capacity was written by Dong, Si;Ma, Sheng;Liu, Zhi-Lin;Ma, Li-Li;Zhang, Yu;Tang, Zhao-Hui;Deng, Ming-Xiao;Song, Wan-Tong. And the article was included in Chinese Journal of Polymer Science in 2021.COA of Formula: C18H17NO5 This article mentions the following:

Poly(2-oxazoline) (POx) is a kind of polymeric amides that can be viewed as conformational isomers of polypeptides with excellent cyto- and hemo-compatibility, and is promising to be used as drug carriers. However, the drug loading capacity (DLC) of POx for many drugs is still low except several hydrophobic ones including paclitaxel (PTX). Herein, we prepared a series of amphiphilic POx block copolymers with various functional groups, and investigated the relationship between functional structures and the DLC. Functional POxs with benzyl, carboxyl, and amino groups in the side-chain were synthesized based on a poly(2-methyl-2-oxazoline)-block-poly(2-butyl-2-oxazoline-co-2-butenyl-2-oxazoline) (PMeOx-P(nBuOx-co-ButenOx), PMBEOx) precursor, followed by click reaction between vinyl and the 2-phenylethanethiol, thioglycolic acid and cysteamine. Using thin-film hydration method, eight commonly used drugs with various characteristics were encapsulated within these functional POx polymers. We found that amine-containing drugs were more easily encapsulated by POx with carboxyl groups, while amine functionalities in POx enhanced the loading capacity of drugs with carboxyl groups. In addition, ;π;π interactions resulted in enhanced DLC of most drugs, except several hydrophobic drugs with aromatic to total carbon ratios less than 0.5. In general, we could successfully encapsulate all the selected drugs with a DLC% over 10% using properly selected functional POxs. The above results confirm that the DLC of polymeric carriers can be adjusted by modifying the functional groups, and the prepared series of functional POxs provide an option for various drug loadings. In the experiment, the researchers used many compounds, for example, 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8COA of Formula: C18H17NO5).

2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8) belongs to amides. Amides include many other important biological compounds, as well as many drugs like paracetamol, penicillin and LSD. Low-molecular-weight amides, such as dimethylformamide, are common solvents. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.COA of Formula: C18H17NO5

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Risk assessment approach for prioritizing danube basin-specific pollutants: a case study in the novi sad region was written by Milovanovic, Dusan;Spanik, Ivan;Miloradov, Mirjana Vojinovic;Mihajlovic, Ivana;Radonic, Jelena;Machynakova, Andrea;Petrovic, Maja. And the article was included in Polish Journal of Environmental Studies in 2019.Electric Literature of C10H16N2O4 This article mentions the following:

The aim of this work was to determine the most relevant pollutants in Danube surface water and wastewater in the city of Novi Sad, and to conduct a risk assessment approach on substances for the optimization of future monitoring programs. According to the requirements of Serbian law, obligatory and operating monitoring was focused on physico-chem. and biol. parameters while the expanded monitoring programs have not been applied – often due to practical circumstances. Novi Sad, with a population of more than 300.000 inhabitants, does not have a wastewater treatment plant, and about 2 m3 of wastewater is discharged directly to the Danube river every day. Screening analyses of the water within the Danube basin around city of Novi Sad included more than 300 different organic substances, while target anal. was conducted for all WFD pollutants. The methodol. for generating the list of priority substances was applied for the first time in the Danubian region around the city of Novi Sad. By applying the prioritization procedure, 86 organic substances were determined in screening analyses and 27 substances obtained within target analyses were identified as the most relevant. In addition, seasonal variation anal. was conducted to determine the occurrence trends of specific compounds in different seasons. In the experiment, the researchers used many compounds, for example, N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4Electric Literature of C10H16N2O4).

N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4) belongs to amides. Because of the greater electronegativity of oxygen, the carbonyl (C=O) is a stronger dipole than the N–C dipole. The presence of a C=O dipole and, to a lesser extent a N–C dipole, allows amides to act as H-bond acceptors. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Electric Literature of C10H16N2O4

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Improving cellulose nanofibrillation of non-wood fiber using alkaline and bleaching pre-treatments was written by Fonseca, Alessandra S.;Panthapulakkal, Suhara;Konar, Samir K.;Sain, Mohini;Bufalino, Lina;Raabe, Joabel;Miranda, Ires Paula A.;Martins, Maria Alice;Tonoli, Gustavo Henrique D.. And the article was included in Industrial Crops and Products in 2019.Product Details of 10543-57-4 This article mentions the following:

This study aimed to investigate the effect of alk. and bleaching pre-treatments on nanofibrillation of jute fibers and on mech. performance of the ensuing cellulose films. Fibers were submitted to mild or strong alk. pre-treatments. Then, only after mild alk. pre-treatment, the fibers were pre-treated with two different bleaching pathways. Succeeding, cellulose nanofibrils were produced by mech. defibrillation of the pre-treated fibers, while cellulose films were obtained by dewatering (suction) of the cellulose suspensions. Com. bleached wood (Eucalyptus and Pinus) pulps were used as reference sources of cellulose fibers. Transmission electron microscopy (TEM) was used to investigate the morphol. of the obtained cellulose nanofibrils. Cellulose films were compared by their tensile properties. Defibrillation process was highly influenced by chem. pre-treatments. By the morphol. analyses, the most effective nanofibrillation was observed for fibers pre-treated with tetraacetylethylenediamine (TAED), leading to nanofibrils with average diameter of around 20 nm. The crystalline index (CI) was higher for nanofibrils from pre-treated fibers (CI > 80%) when compared to the raw fibers (CI = 61%). Jute fibers pre-treated with TAED bleaching led to higher nanofibrillation, resulting on films with higher tensile strength and Young’s modulus, which were similar to mech. properties of those from com. wood pulp. In the experiment, the researchers used many compounds, for example, N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4Product Details of 10543-57-4).

N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.Product Details of 10543-57-4

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Controlled synthesis of N,N-dimethylarylsulfonamide derivatives as nematicidal agents was written by Chen, Gen-Qiang;Xia, Yan-Fei;Yang, Jin-Ming;Che, Zhi-Ping;Sun, Di;Li, Shen;Tian, Yue-E.;Liu, Sheng-Ming;Jiang, Jia;Lin, Xiao-Min. And the article was included in Journal of Asian Natural Products Research in 2020.HPLC of Formula: 383-31-3 This article mentions the following:

Gramine could be intelligently and efficiently supplied with N,N-dimethylamino group and then reacted with the corresponding sulfonyl chlorides to synthesize N,N-dimethylarylsulfonamides I [R = Ph, 4-FC₆H₄, 2-thienyl, etc.]. Herein designed and controlled synthesis of N,N-dimethylarylsulfonamide derivatives and first reported the results of the nematicidal activity of N,N-dimethylarylsulfonamide compounds against Meloidogyne incongnita in vitro, resp. Among all of the N,N-dimethylarylsulfonamide derivatives, compounds I [R = Ph, 4-MeOC₆H₄, 4-O₂NC₆H₄, 8-quinolyl] exhibited potent nematicidal activity with median lethal concentration (LC₅₀) values ranging from 0.22 to 0.26 mg/L. Most noteworthy, compounds I [R = 4-MeOC₆H₄, 8-quinolyl] showed the best promising and pronounced nematicidal activity, with LC₅₀ values of 0.2381 and 0.2259 mg/L, resp. In the experiment, the researchers used many compounds, for example, 4-Fluoro-N,N-dimethylbenzenesulfonamide (cas: 383-31-3HPLC of Formula: 383-31-3).

4-Fluoro-N,N-dimethylbenzenesulfonamide (cas: 383-31-3) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.HPLC of Formula: 383-31-3

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Nanomicelle-generating microneedles loaded with tranilast for treatment of hypertrophic scars in a rabbit model was written by Chien, Pham Ngoc;Jeong, Jae Heon;Nam, Sun Young;Lim, Su Yeon;Van Long, Nguyen;Zhang, Xin Rui;Jeong, Ji Hoon;Heo, Chan Yeong. And the article was included in In Vivo in 2022.Category: amides-buliding-blocks This article mentions the following:

Background/Aim: Hypertrophic scars (HS) are the result of pathol. wound healing characterized by a red, raised scar formation. The goal of this research was development of a new method for treatment of HS formation. Materials and Methods: A tranilast-loaded microneedle (TMN) was developed and applied in a rabbit ear model to treat an induced HS. Scar elevation index, the thickness of dorsal skin by hematoxylin and eosin staining, collagen deposition by Masson trichrome staining and expression of myofibroblast biomarker proteins were evaluated. Results: The 12×12 array of the TMN containing 2.9 μg tranilast per needle released more than 80% of the drug within 30 min. During the procedure, control, non-loaded MN and TMN loaded with three different doses of tranilast (low: 2.5-3, medium: 25-30, and high: 100-150 μg) were applied to the HS in rabbit ears. High-level TMN led to a clear and natural appearance of skin, a decrease in scar elevation index by 47% and decline in the thickness of the epidermis from 69.27 to 15.92 μm when compared to the control group. Moreover, the collagen d. also decreased in groups treated with medium- or high-level TMNs, by 10.2% and 9.06%, resp. Furthermore, the expression of transforming growth factor-β, collagen-1, and α-smooth muscle actin proteins was reduced in TMN-treated HSs compared to the control. Conclusion: The findings show the overall efficacy of TMNs in inhibiting HS. Thus, use of TMN is a simple and cosmetic remedy for HS, with good protection and reliability. In the experiment, the researchers used many compounds, for example, 2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8Category: amides-buliding-blocks).

2-(3-(3,4-Dimethoxyphenyl)acrylamido)benzoic acid (cas: 53902-12-8) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. The presence of the amide group –C(=O)N– is generally easily established, at least in small molecules. It can be distinguished from nitro and cyano groups in IR spectra. Amides exhibit a moderately intense νCO band near 1650 cm−1. By 1H NMR spectroscopy, CONHR signals occur at low fields. In X-ray crystallography, the C(=O)N center together with the three immediately adjacent atoms characteristically define a plane.Category: amides-buliding-blocks

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

α-Keto Amides and 1,2-Diketones from N,N’-Dimethoxy-N,N’-dimethylethanediamide. A Synthetic and Mechanistic Investigation was written by Sibi, Mukund P.;Marvin, Mali;Sharma, Rajiv. And the article was included in Journal of Organic Chemistry in 1995.Reference of 106675-70-1 This article mentions the following:

Title diamide (MeONMeCO)₂ (1), a 1,2-dicarbonyl synthon prepared from oxalyl chloride, undergoes nucleophilic displacements with Grignard reagents to provide α-keto amides in 28-90% yields. The synthon also undergoes double nucleophilic displacements with organolithium reagents to furnish sym. 1,2-diketones in 15-84% yields. A mechanism accounting for all the products from the reaction of 1 with nucleophiles has been proposed. Several control experiments were carried out to support the proposed mechanism. In the experiment, the researchers used many compounds, for example, N1,N2-Dimethoxy-N1,N2-dimethyloxalamide (cas: 106675-70-1Reference of 106675-70-1).

N1,N2-Dimethoxy-N1,N2-dimethyloxalamide (cas: 106675-70-1) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.Reference of 106675-70-1

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Construction of triblock copolyesters via one-step switchable terpolymerization of epoxides, phthalic anhydride and ε-caprolactone using dual urea/organic base catalysts was written by Lin, Limiao;Chen, Xin;Xiang, Huanxin;Chang, Min;Xu, Yonghang;Zhao, Hongting;Meng, Yuezhong. And the article was included in Polymer Chemistry in 2022.Quality Control of 1,3-Dicyclohexylurea This article mentions the following:

In this study, triblock copolyesters were synthesized via a switchable terpolymn. of mixed monomers of epoxides, phthalic anhydride (PA) and ε-caprolactone (CL) by using cost-effective dual urea/organic base catalysts. Different combinations of urea/organic base pairs were evaluated in order to optimize the catalytic activity and switchable selectivity, and different types of epoxides were employed to obtain diverse copolyesters. The sequence structures of the obtained copolyesters were analyzed by gel permeation chromatog. and diffusion-ordered NMR spectroscopy (DOSY) measurements. The switchable mechanism of the ROP of lactones and ROAC of epoxide/anhydride cycles was investigated via in situ anal. of ¹H NMR, GPC and ATR-IR spectroscopy. The results showed that the N,N’-dicyclohexylurea (U1)/bis(triphenylphosphine)iminium chloride (PPNCl) pair with more alk. urea exhibited the highest catalytic activity for the mixture of cyclohexene oxide, PA and CL in both ring-opening alternating copolymerization (ROAC) and ring-opening polymerization (ROP) reaction cycles as compared to those previously reported. It was found that the existence/exhaustion of PA could be considered to be the switch between ROAC and ROP reaction cycles, and that the superior catalytic selectivity contributed to the successful formation of the desired triblock sequence structures. In the experiment, the researchers used many compounds, for example, 1,3-Dicyclohexylurea (cas: 2387-23-7Quality Control of 1,3-Dicyclohexylurea).

1,3-Dicyclohexylurea (cas: 2387-23-7) belongs to amides. The solubilities of amides and esters are roughly comparable. Typically amides are less soluble than comparable amines and carboxylic acids since these compounds can both donate and accept hydrogen bonds. Tertiary amides, with the important exception of N,N-dimethylformamide, exhibit low solubility in water. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Quality Control of 1,3-Dicyclohexylurea

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Exploiting a “Beast” in Carbenoid Chemistry: Development of a Straightforward Direct Nucleophilic Fluoromethylation Strategy was written by Parisi, Giovanna;Colella, Marco;Monticelli, Serena;Romanazzi, Giuseppe;Holzer, Wolfgang;Langer, Thierry;Degennaro, Leonardo;Pace, Vittorio;Luisi, Renzo. And the article was included in Journal of the American Chemical Society in 2017.Quality Control of N-Methoxy-N-methyl-4-(trifluoromethyl)benzamide This article mentions the following:

The first direct and straightforward nucleophilic fluoromethylation of organic compounds is reported. The tactic employs a “fleeting” lithium fluorocarbenoid (LiCH₂F) generated from com. available fluoroiodomethane. Precise reaction conditions were developed for the generation and synthetic exploitation of such a labile species. The versatility of the strategy is showcased in ca. 50 examples involving a plethora of electrophiles. Highly valuable chems. such as fluoroalcs., fluoroamines, and fluoromethylated oxygenated heterocycles could be prepared in very good yields through a single synthetic operation. The scalability of the reaction and its application to complex mol. architectures (e.g., steroids) are documented. PhSOCH₂F. In the experiment, the researchers used many compounds, for example, N-Methoxy-N-methyl-4-(trifluoromethyl)benzamide (cas: 116332-61-7Quality Control of N-Methoxy-N-methyl-4-(trifluoromethyl)benzamide).

N-Methoxy-N-methyl-4-(trifluoromethyl)benzamide (cas: 116332-61-7) belongs to amides. Amides include many other important biological compounds, as well as many drugs like paracetamol, penicillin and LSD. Low-molecular-weight amides, such as dimethylformamide, are common solvents. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Quality Control of N-Methoxy-N-methyl-4-(trifluoromethyl)benzamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

RVX-208, an inhibitor of BET transcriptional regulators with selectivity for the second bromodomain was written by Picaud, Sarah;Wells, Christopher;Felletar, Ildiko;Brotherton, Deborah;Martin, Sarah;Savitsky, Pavel;Diez-Dacal, Beatriz;Philpott, Martin;Bountra, Chas;Lingard, Hannah;Fedorov, Oleg;Muller, Susanne;Brennan, Paul E.;Knapp, Stefan;Filippakopoulos, Panagis. And the article was included in Proceedings of the National Academy of Sciences of the United States of America in 2013.SDS of cas: 63920-73-0 This article mentions the following:

Bromodomains have emerged as attractive candidates for the development of inhibitors targeting gene transcription. Inhibitors of the bromo and extraterminal (BET) family recently showed promising activity in diverse disease models. However, the pleiotropic nature of BET proteins regulating tissue-specific transcription has raised safety concerns and suggested that attempts should be made for domain-specific targeting. Here, we report that RVX-208, a compound currently in phase II clin. trials, is a BET bromodomain inhibitor specific for second bromodomains (BD2s). Cocrystal structures revealed binding modes of RVX-208 and its synthetic precursor, and fluorescent recovery after photobleaching demonstrated that RVX-208 displaces BET proteins from chromatin. However, gene-expression data showed that BD2 inhibition only modestly affects BET-dependent gene transcription. Our data demonstrate the feasibility of specific targeting within the BET family resulting in different transcriptional outcomes and highlight the importance of BD1 in transcriptional regulation. In the experiment, the researchers used many compounds, for example, 2-Amino-4,6-dimethoxybenzamide (cas: 63920-73-0SDS of cas: 63920-73-0).

2-Amino-4,6-dimethoxybenzamide (cas: 63920-73-0) belongs to amides. Amides include many other important biological compounds, as well as many drugs like paracetamol, penicillin and LSD. Low-molecular-weight amides, such as dimethylformamide, are common solvents. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.SDS of cas: 63920-73-0

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics