In Vitro Cytotoxic Activity of Clinacanthus nutans Leaf Extracts Against HeLa Cells was written by Haron, Nor Hasyimah;Toha, Zaleha Md;Abas, Rafedah;Hamdan, Mohammad Razak;Azman, Nizuwan;Khairuddean, Melati;Arsad, Hasni. And the article was included in Asian Pacific Journal of Cancer Prevention in 2019.Related Products of 2387-23-7 This article mentions the following:
This study was conducted to investigate the antiproliferative activity of extracts of Clinacanthus nutans leaves against human cervical cancer (HeLa) cells. C. nutans leaves were subjected to extraction using 80% methanol or water. The methanol extract was further extracted to obtain hexane, dichloromethane (DCM), and aqueous fractions. The antiproliferative activity of the extracts against HeLa cells was determined The most cytotoxic extract was furthered analyzed by apoptosis and cell cycle assays, and the phytochem. constituents were screened by gas chromatog.-mass spectrometry (GC-MS). All of the extracts were antiproliferative against HeLa cells, and the DCM fraction had the lowest IC 50 value of 70 μg/mL at 48 h. Microscopic studies showed that HeLa cells exposed to the DCM fraction exhibited marked morphol. features of apoptosis. The flw cytometry study also confimed that the DCM fraction induced apoptosis in HeLa cells, with cell cycle arrest at the S phase. GC-MS anal. revealed the presence of at least 28 compounds in the DCM fraction, most of which were fatty acids. The DCM fraction obtained using the extraction method described herein had a lower IC50 value than those reported in previous studies that characterized the anticancer activity of C. nutans against HeLa cells. In the experiment, the researchers used many compounds, for example, 1,3-Dicyclohexylurea (cas: 2387-23-7Related Products of 2387-23-7).
1,3-Dicyclohexylurea (cas: 2387-23-7) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.Related Products of 2387-23-7
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics