Validation of the CULTEX Radial Flow System for the assessment of the acute inhalation toxicity of airborne particles was written by Tsoutsoulopoulos, Amelie;Gohlsch, Katrin;Moehle, Niklas;Breit, Andreas;Hoffmann, Sebastian;Krischenowski, Olaf;Mueckter, Harald;Gudermann, Thomas;Thiermann, Horst;Aufderheide, Michaela;Steinritz, Dirk. And the article was included in Toxicology In Vitro in 2019.COA of Formula: C10H16N2O4 This article mentions the following:
The CULTEX Radial Flow System (RFS) is a modular in vitro system for the homogenous exposure of cells to airborne particles at the air-liquid interface (ALI). A former pre-validation study successfully demonstrated the general applicability of the CULTEX RFS and its transferability, stability and reproducibility. Based on these results, the methodol. was optimized, validated and prediction models for acute inhalation hazards were established. Cell viability of A549 cells after ALI exposure to 20 pre-selected test substances was assessed in three independent laboratories Cytotoxicity of test substances was compared to the resp. incubator controls and used as an indicator of toxicity. Substances were considered to exert an acute inhalation hazard when viability decreased below 50% (prediction model (PM) 50%) or 75% (PM 75%) at any of three exposure doses (25, 50 or 100 μg/cm2). Results were then compared to existing in vivo data and revealed an overall concordance of 85%, with a specificity of 83% and a sensitivity of 88%. Depending on the applied PM, the within-laboratory and between-laboratory reproducibility ranged from 90 to 100%. In summary, the CULTEX RFS was proven as a transferable, reproducible and well predictive screening method for the qual. assessment of the acute pulmonary cytotoxicity of airborne particles. In the experiment, the researchers used many compounds, for example, N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4COA of Formula: C10H16N2O4).
N,N-(Ethane-1,2-diyl)bis(N-acetylacetamide) (cas: 10543-57-4) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.COA of Formula: C10H16N2O4
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics