Mostafavi, Mahdiyeh et al. published their research in Biochemical Engineering Journal in 2022 | CAS: 10238-21-8

5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides are not in general accessible by the direct condensation of amines with carboxylic acids for two reasons: first, both components are readily deactivated by a transfer of a proton from the acid to the amine and second, the hydroxy unit on the carbonyl of the acid is a relatively poor leaving group. Nevertheless, the formation of five- and six-membered rings is often surprisingly simple provided that other factors can be brought into play to assist in the condensation.Electric Literature of C23H28ClN3O5S

Development of a bioluminescence assay for BIR2- caspase3 interaction through split luciferase complementary assay was written by Mostafavi, Mahdiyeh;Ataei, Farangis;Hamidieh, Amir Ali;Hosseinkhani, Saman. And the article was included in Biochemical Engineering Journal in 2022.Electric Literature of C23H28ClN3O5S This article mentions the following:

Harnessing of firefly luciferase biochem. enabled to develop bioluminescent assay for different intracellular protein-protein interactions. Caspase-3 and XIAP are two important players in maintaining the balance of cell death and survival. Linker-BIR2 from XIAP is known to be sufficient for inhibition of caspase-3. Here, caspase-3 and Linker-BIR2 were used for designing two reporters based on split luciferase complementary assay to develop a luminescent probe. Caspase3-CLuc and BIR2-NLuc fragments were ligated in pET-28a (+), overexpressed in E. coli BL21 (DE3) and purified to homogeneity. Interaction between caspase-3 and BIR2 was evaluated by different exptl. methods such as luciferase and caspase-3 activity, directed mutagenesis and ELISA, which approved their specific interaction. Next, the developed reporters were employed for analyzing four compounds (Sulphadimidin, Glyburide, Carboplatin and Cetirizine) that showed effects on caspase-3 and BIR2 interaction with the largest effect for Carboplatin. Mol. docking was also showed the largest binding affinity for Glyburide. In conclusion, the developed assay showed specific interaction with light generation, and ability to analyze some chems. in vitro. In the experiment, the researchers used many compounds, for example, 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8Electric Literature of C23H28ClN3O5S).

5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides are not in general accessible by the direct condensation of amines with carboxylic acids for two reasons: first, both components are readily deactivated by a transfer of a proton from the acid to the amine and second, the hydroxy unit on the carbonyl of the acid is a relatively poor leaving group. Nevertheless, the formation of five- and six-membered rings is often surprisingly simple provided that other factors can be brought into play to assist in the condensation.Electric Literature of C23H28ClN3O5S

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics