Quantification of drugs encapsulated in liposomes by 1H NMR was written by Guimaraes, Diana;Noro, Jennifer;Loureiro, Ana;Cavaco-Paulo, Artur;Nogueira, Eugenia. And the article was included in Colloids and Surfaces, B: Biointerfaces in 2019.HPLC of Formula: 7413-34-5 This article mentions the following:
Liposomes are one of the most important and extensively studied drug delivery system due to their ability to encapsulate different kinds of drugs. Exploiting the advantages of 1H NMR (NMR) spectrometry, we established a rapid and easy method for quantification of drugs encapsulated in liposomes. An internal standard, pyridine, was used for quant. determination of drug concentration Two different drugs were involved in this work, one hydrophilic, methotrexate disodium salt, and another hydrophobic, tamoxifen. The specificity and selectivity of the suggested method were evaluated by the absence of overlapping of at least one signal of each drug with pyridine in the NMR spectrum. The accuracy and precision of the method were assessed by adding a known amount of each drug to unloaded liposomes. Results obtained by quant. NMR (qNMR) were validated and confirmed by comparing with two other traditional techniques, UV-Visible (UV-vis) spectrophotometry and High-Performance Liquid Chromatog. (HPLC). It was found that the results were consistent with the ones obtained from our proposed qNMR method. Considering all the experiments conducted in this study, we deliberate that qNMR can be a suitable tool for the determination of drugs encapsulated in liposomes. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5HPLC of Formula: 7413-34-5).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. The solubilities of amides and esters are roughly comparable. Typically amides are less soluble than comparable amines and carboxylic acids since these compounds can both donate and accept hydrogen bonds. Tertiary amides, with the important exception of N,N-dimethylformamide, exhibit low solubility in water. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.HPLC of Formula: 7413-34-5
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics