Neuropharmacological effects of methotrexate perfused through the cerebrospinal fluid system of the Rhesus monkey was written by Shepard, Robert F.;Merker, Philip C.;Walker, Michael D.;Gilles, Floyd H.. And the article was included in Cancer Research in 1976.Safety of Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate This article mentions the following:
Thirteen adult Rhesus monkeys were repeatedly perfused through the ventriculocisternal or ventriculolumbar spaces with Elliott’s B solution containing various concentrations of Na methotrexate (I Na salt) [7413-34-5] and trace amounts of 3H-I and 14C-inulin. The concentrations of I ranged from 4.8 to 0.15 mg/ml representing perfusion dosages of 551 to 16 mg/m2. The average steady-state concentration out-concentration in (Co/Ci) value for I was 0.78 for the ventriculocisternal and 0.66 for the ventriculolumbar routes. I treatments did not significantly affect mean inulin steady-state Co/Ci values or CSF formation rate. With the exception of a monkey perfused with I at an inflow concentration of 4.8 mg/ml, body weight, food intake, and urine output, analyzed at weekly intervals, generally were not remarkably affected by I perfusions. In 5 monkeys perfused with I in concentrations of 4.8 to 0.6 mg/ml, gross neurol. toxicity was observed, principally in the form of seizures and hypokinesia during perfusion series with occasional residual motor deficit. Significant cerebral damage was associated with the brains of two monkeys perfused with I at concentrations of 2.4 and 0.6 mg/ml and 2 monkeys perfused at concentrations of 1.2 and 0.3 mg/ml; 3 of the 4 animals displayed signs of gross neurotoxicity, and 2 animals developed permanent motor deficits. However, the extent to which neurotoxic signs could be attributed solely to I was difficult to judge because some changes in central nervous system morphol. were associated with the mechanical aspects of the procedure. Overall behavioral performance as measured by a visual pattern discrimination reinforced by avoidance or escape from an elec. shock was not significantly affected by repeated perfusions of I (0.6 mg/ml) in 2 monkeys not otherwise studied in detail. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Safety of Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. Amides include many other important biological compounds, as well as many drugs like paracetamol, penicillin and LSD. Low-molecular-weight amides, such as dimethylformamide, are common solvents. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Safety of Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate
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Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics