Inhibition of methotrexate transport from cerebrospinal fluid by probenecid was written by Spector, Reynold;Levy, Peter. And the article was included in Cancer Treatment Reports in 1976.Reference of 7413-34-5 This article mentions the following:
In rabbits, the efflux of intraventricularly injected Na methotrexate (I Na salt) [7413-34-5] from the cerebrospinal fluid was retarded by pretreatment with probenecid [57-66-9] (200 mg/kg, i.p.). In vitro, the ability of the isolated choroid plexus to concentrate methotrexate was depressed by the inclusion of probenecid in the incubation medium. These exptl. results are consistent with the hypothesis that probenecid depresses the clearance of methotrexate from the cerebrospinal fluid by blocking the transport of methotrexate from cerebrospinal fluid to blood via the choroid plexus. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Reference of 7413-34-5).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. Amides can be viewed as a derivative of a carboxylic acid RC(=O)OH with the hydroxyl group –OH replaced by an amine group −NR′R″; or, equivalently, an acyl (alkanoyl) group RC(=O)− joined to an amine group. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Reference of 7413-34-5
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics