The effect of methotrexate on chemical carcinogenesis of hamster buccal pouch was written by Shklar, Gerald;Cataldo, Edmund;Fitzgerald, Arthur L.. And the article was included in Cancer Research in 1966.Electric Literature of C20H20N8Na2O5 This article mentions the following:
Na methotrexate, injected subcutaneously 3 times weekly at 0.0625 mg., on the same days that the right buccal pouch was painted with 0.5% 9,10-dimethyl-1,2-benzanthracene, augmented neoplastic formation induced by the carcinogen in hamsters. The tumors were also more anaplastic and of greater size in the group given Na methotrexate. Na methotrexate was not carcinogenic by itself, and the drug treatments did not result in toxicity, decreased food consumption, or loss of body weight 16 references. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Electric Literature of C20H20N8Na2O5).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. Compared to amines, amides are very weak bases and do not have clearly defined acid–base properties in water. On the other hand, amides are much stronger bases than esters, aldehydes, and ketones. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Electric Literature of C20H20N8Na2O5
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics