Acute toxicity of methotrexate and cyclophosphamide in underfed mice was written by Graczyk, Julita. And the article was included in Bromatologia i Chemia Toksykologiczna in 1980.Synthetic Route of C20H20N8Na2O5 This article mentions the following:
The i.p. LD50 values of Na methotrexate (I Na salt) [7413-34-5] and cyclophosphamide (II) [50-18-0] in mice fed normal diets were 122 and 460 mg/kg, resp. In animals maintained on 10 and 4% protein diets, the toxicity of I was increased by 3.5- and 20-fold, resp., and that of II was increased by 4- and 8-fold, resp. At LD10 doses, I and II decreased serum total proteins levels in mice on normal diets from 72.3 to 49.1 and 46.8 g/dm3, resp. The latter result may possibly explain the enhancement of I and II toxicity by protein-deficient diets. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Synthetic Route of C20H20N8Na2O5).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides are not in general accessible by the direct condensation of amines with carboxylic acids for two reasons: first, both components are readily deactivated by a transfer of a proton from the acid to the amine and second, the hydroxy unit on the carbonyl of the acid is a relatively poor leaving group. Nevertheless, the formation of five- and six-membered rings is often surprisingly simple provided that other factors can be brought into play to assist in the condensation.Synthetic Route of C20H20N8Na2O5
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics