Pinto, Brunella published the artcileSynthesis and label free characterization of a bimolecular PNA homo quadruplex, Formula: C40H35N7O8, the publication is Biochimica et Biophysica Acta, General Subjects (2017), 1861(5_Part_B), 1222-1228, database is CAplus and MEDLINE.
G-quadruplex DNA is involved in many physiol. and pathol. processes. Both clin. and exptl. studies on DNA G-quadruplexes are slowed down by their enzymic instability. In this frame, more stable chem. modified analogs are needed. The bis-end-linked-(gggt)2 PNA mol. (BEL-PNA) was synthesized using in solution and solid phase synthetic approaches. Quadruplex formation was assessed by CD (CD) and surface enhanced Raman scattering (SERS). An unprecedented bimol. PNA homo quadruplex is here reported. To achieve this goal, we developed a bifunctional linker that once functionalized with gggt PNA strands and annealed in K+ buffer allowed the obtainment of a PNA homo quadruplex. 4The identification of the strong SERS band at ∼ 1481 cm– 1, attributable to vibrations involving the quadruplex diagnostic Hoogsteen type hydrogen bonds, confirmed the formation of the PNA homo quadruplex. By tethering two G-rich PNA strands to the two ends of a suitable bifunctional linker it is possible to obtain bimol. PNA homo quadruplexes after annealing in K+-containing buffers. The formation of such CD-unfriendly complexes can be monitored, even at low concentrations, by using the SERS technique. Given the importance of DNA G-quadruplexes in medicine and nanotechnol., the obtainment of G-quadruplex analogs provided with enhanced enzymic stability, and their monitoring by highly sensitive label-free techniques are of the highest importance. This article is part of a Special Issue entitled “G-quadruplex” – guest edited by Dr. Concetta Giancola and Dr. Daniela Montesarchio.
Biochimica et Biophysica Acta, General Subjects published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Formula: C40H35N7O8.
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https://en.wikipedia.org/wiki/Amide,
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