Smith, Christine C. published the artcileThe synthesis and application of a diazirine-modified uridine analogue for investigating RNA-protein interactions, COA of Formula: C6H10F3NO, the publication is RSC Advances (2014), 4(89), 48228-48235, database is CAplus.
The roles played by many ncRNAs remain largely unknown. Similarly, relatively little is known about the RNA binding proteins involved in processing ncRNA. Identification of new RNA/RNA binding protein (RBP) interactions may pave the way to gain a better understanding of the complex events occurring within cells during gene expression and ncRNA biogenesis. The development of chem. tools for the isolation of RBPs is of paramount importance. In this context, we report on the synthesis of the uridine phosphoramidite UDz that bears a diazirine moiety on the nucleobase. RNA probes containing UDz units were irradiated in the presence of single-stranded DNA binding protein (SSB), which is also known to bind ssRNAs, and shown to efficiently (15% yield) and selectively cross-link to the protein. The corresponding diazirine-modified uridine triphosphate UDzTP was synthesized and its capacity to act as a substrate for the T7 RNA polymerase was tested in transcription assays. UDzTP was accepted with a maximum yield of 38% for a 26mer RNA containing a single incorporation and 28% yield for triple consecutive incorporations. Thus, this uridine analog represents a convenient biochem. tool for the identification of RNA binding proteins and unraveling the role and function played by ncRNAs.
RSC Advances published new progress about 360-92-9. 360-92-9 belongs to amides-buliding-blocks, auxiliary class Trifluoromethyl,Fluoride,Amine,Aliphatic hydrocarbon chain,Amide, name is N,N-Diethyl-2,2,2-trifluoroacetamide, and the molecular formula is C7H13ClNNaO5S, COA of Formula: C6H10F3NO.
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