Bruun, L. published the artcileA quantitative enzyme-linked immunoassay for the detection of 2,6-dichlorobenzamide (BAM), a degradation product of the herbicide dichlobenil, Name: 2,4-Dichlorobenzamide, the publication is Journal of Immunological Methods (2000), 240(1-2), 133-142, database is CAplus and MEDLINE.
2,6-Dichlorobenzamide (BAM) is the dominant degradation product in soil of the widely used herbicide dichlobenil. To detect BAM in water, a highly sensitive and specific ELISA was developed. As an alternative to conventional coating of ELISA plates, the assay is based on direct covalent immobilization. A surface was achieved which requires a short time for the immobilization of ligand, is stable under dry storage, and which permits assays with a low CV. The performance of the assay was demonstrated by an inter-well CV that was generally less than 6%, a detection limit (DL15) of 0.02 μg/l and an IC50 of 0.19 μg/l. Cross-reactivity was measured against nine analytes with structural homol. to BAM. The highest degree of cross-reactivity (10.8%) was seen with 2,6-dichlorothiobenzamide (Chlorthiamid). Considering an EU-limit of 0.1 μg/l as the permissible maximum for the presence of pesticides in drinking water, this ELISA-procedure is suitable for large-scale screening of water samples suspected of being contaminated with BAM.
Journal of Immunological Methods published new progress about 2447-79-2. 2447-79-2 belongs to amides-buliding-blocks, auxiliary class Chloride,Amine,Benzene,Amide, name is 2,4-Dichlorobenzamide, and the molecular formula is C7H5Cl2NO, Name: 2,4-Dichlorobenzamide.
Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics