Fractionation of the photochemical systems of photosynthesis. I. Chlorophyll contents and photochemical activities of particles isolated from spinach chloroplasts was written by Anderson, Jan M.;Boardman, N. K.. And the article was included in Biochimica et Biophysica Acta, Biophysics Including Photosynthesis in 1966.Reference of 730-25-6 The following contents are mentioned in the article:
Spinach chloroplasts were fragmented by incubation with digitonin and the resulting chlorophyll-containing particles separated by differential centrifugation. Particles pelleted by forces of 1000 g and 10,000 g had a lower ratio of chlorophyll a/chlorophyll b (chl a/chl b) than the chloroplasts, whereas the smaller particles which either sedimented at 50,000-144,000 g or remained in the 144,000-g supernatant had higher ratios than the chloroplasts. Chl a/chl b ratios were usually determined spectrophotometrically in 80% acetone, but essentially the same ratios were obtained after transfer of the pigments to ether or after separation of the chlorophylls by thin-layer chromatography. The small particles were inactive in the Hill reaction, either with ferricyanide, trichloroindophenol, or NADP as oxidant, but they photoreduced NADP if provided with the electron-donor couple, Na ascorbate, and dichloroindophenol, and both ferredoxin and NADP reductase. The 1000-g and 10,000-g fractions showed Hill activity, but the rate of reduction of NADP in the presence of ferredoxin and NADP reductase was lower than the rates of reduction of ferricyanide and trichloroindophenol. The particles with the high chl a/chl b ratios appear to be representative of System I (the “long wavelength” system) of photosynthesis, whereas the 1000-g and 10,000-g fractions appear to be enriched in particles representative of System 2. Chloroplasts were also fragmented by Triton X-100 and Nonidet P-40; in contrast to the digiton treatment, the particles so obtained were photochem. inactive. This study involved multiple reactions and reactants, such as N-(3,4-Dichlorophenyl)octanamide (cas: 730-25-6Reference of 730-25-6).
N-(3,4-Dichlorophenyl)octanamide (cas: 730-25-6) belongs to amides. Compared to amines, amides are very weak bases and do not have clearly defined acid–base properties in water. On the other hand, amides are much stronger bases than esters, aldehydes, and ketones. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Reference of 730-25-6
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics