Sadutto, Daniele published the artcileAnalytical and semipreparative high performance liquid chromatography enantioseparation of bicalutamide and its chiral impurities on an immobilized polysaccharide-based chiral stationary phase, Name: N-(4-Cyano-3-(trifluoromethyl)phenyl)-3-((2-fluorophenyl)sulfonyl)-2-hydroxy-2-methylpropanamide, the main research area is liquid chromatog enantioseparation bicalutamide chiral impurity polysaccharide; Bicalutamide; Chiralpak IA; Enantiomers; Impurities; Semipreparative resolution; Temperature-variable HPLC.
Direct HPLC separation of enantiomers of Bicalutamide (BCT), a non-steroidal antiandrogen used for the treatment of prostate cancer, was performed by using the immobilized amylose-based Chiralpak IA chiral stationary phase (CSP). Enantioselective conditions were achieved using standard normal phase mixtures n-hexane-alc. (ethanol or 2-propanol) and a “”non-standard”” mobile phase containing Et acetate (EA). The chromatog. behavior of the IA CSP under these elution modes was evaluated and compared at different temperatures The eluent mixture n-hexane-EA-ethanol 100-30-5 (volume/volume/v) and the column temperature of 40 °C were identified as the best operational conditions to carry out semipreparative enantioseparations on a 1-cm I. D. IA column. Using this protocol, about 960 mg of (R)-BCT, which is the enantiomer with the almost entire anti-androgenic activity of BCT, per day could be isolated. The anal. and semipreparative HPLC resolution of chiral impurities of BCT, and their empiric absolute configuration assignment by CD correlation method are also presented.
Journal of Chromatography A published new progress about Chiral resolution. 1159977-36-2 belongs to class amides-buliding-blocks, name is N-(4-Cyano-3-(trifluoromethyl)phenyl)-3-((2-fluorophenyl)sulfonyl)-2-hydroxy-2-methylpropanamide, and the molecular formula is C18H14F4N2O4S, Name: N-(4-Cyano-3-(trifluoromethyl)phenyl)-3-((2-fluorophenyl)sulfonyl)-2-hydroxy-2-methylpropanamide.
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics