Lu, Nan published the artcileNon-aqueous electrophoresis integrated with electrospray ionization mass spectrometry on a thiol-ene polymer-based microchip device, Synthetic Route of 123-39-7, the main research area is electrophoresis integrated electrospray ionization mass spectrometry thiol ene polymer; microchip device; Electrospray interface; Microfluidics; Non-aqueous electrophoresis; Thiol-ene polymers.
Abstract: Non-aqueous capillary electrophoresis (NACE) on microfluidic chips is still a comparatively little explored area, despite the inherent advantages of this technique and its application potential for, in particular, lipophilic compounds A main reason is probably the fact that implementation of NACE on microchips largely precluded the use of polymeric substrate materials. Here, we report non-aqueous electrophoresis on a thiol-ene-based microfluidic chip coupled to mass spectrometry via an on-chip ESI interface. Microchips with an integrated ESI emitter were fabricated using a double-molding approach. The durability of thiol-ene, when exposed to different organic solvents, was investigated with respect to swelling and decomposition of the polymer. Thiol-ene exhibited good stability against organic solvents such as methanol, ethanol, N-methylformamide, and formamide, which allows for a wide range of background electrolyte compositions The integrated ESI emitter provided a stable spray with RSD% of the ESI signal ≤8%. Separation efficiency of the developed microchip electrophoresis system in different non-aqueous buffer solutions was tested with a mixture of several drugs of abuse. Ethanol- and methanol-based buffers provided comparable high theor. plate numbers (≈ 6.6 x 104-1.6 x 105 m-1) with ethanol exhibiting the best separation efficiency. Direct coupling of non-aqueous electrophoresis to mass spectrometry allowed for fast anal. of hydrophobic compounds in the range of 0.1-5μg mL-1 and 0.2-10μg mL-1 and very good sensitivities (LOD ≈ 0.06-0.28μg mL-1; LOQ ≈ 0.20-0.90μg mL-1). The novel combination of non-aqueous CE on a microfluidic thiol-ene device and ESI-MS provides a mass-producible and highly versatile system for the anal. of, in particular, lipophilic compounds in a wide range of organic solvents. This offers promising potential for future applications in forensic, clin., and environmental anal.
Analytical and Bioanalytical Chemistry published new progress about Electrophoresis. 123-39-7 belongs to class amides-buliding-blocks, name is N-Methylformamide, and the molecular formula is C2H5NO, Synthetic Route of 123-39-7.
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