Perry, Carolina Trindade published the artcileAmides as cryoprotectants for the freezing of Brycon orbignyanus sperm, Safety of N-Methylformamide, the main research area is Brycon sperm freezing amide cryoprotectant.
The objective of this study was to evaluate the use of different amides as an alternative for the cryopreservation of sperm from the endangered species, Bryconorbignyamus, an important fish for aquaculture in Brazil. The cryoprotectants which were combined with Beltsville Thawing Solution extender, each at the concentrations of 2.5%, 5%, 7.5%, and 10%, were as follows: (1) dimethylacetamide (DMA), (2) DMF (DMF), and (3) methylformamide (MF). The cyoprotectant methylglycol (MG, 10%) was used as a control group. Therefore, 13 treatment groups were performed. In order to assess DNA fragmentation index, lipid peroxidation, cell integrity, integrity of plasma membrane, membrane fluidity, mitochondrial functionality, and reactive oxygen species, flow cytometry was used. The best results were obtained with 7.5% DMF, among all the amides tested, with sperm motility (66%) significantly higher than the other cryoprotectants evaluated (P < .05). It also exhibited high membrane integrity (84.5%) and cell integrity (98.7%), and low membrane fluidity (20.3%) (P < .05). MG (10%) exhibited only 38% motility, lower mitochondrial protection (39.8%), and higher DNA fragmentation index (0.07), despite the high membrane integrity observed (85.6%). The DMF concentration of 7.5% demonstrated greater protection of sperm function and cellular structure, and is therefore recommended for the cryopreservation of B. orbignyamus spermatozoa. Aquaculture published new progress about Amides Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 123-39-7 belongs to class amides-buliding-blocks, name is N-Methylformamide, and the molecular formula is C2H5NO, Safety of N-Methylformamide.
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics