On September 25, 2013, Xiao, Run-mei; Sun, Jing-zhi; Mei, Yong; Liu, Jie; Chen, Yong published an article.COA of Formula: C10H11NO3 The title of the article was Simultaneous determination of four urinary metabolites of hippuric acid and methyl hippuric acid by HPLC. And the article contained the following:
A high performance liquid chromatog.(HPLC) method with diode array detection was established for simultaneous determination of hippuric acid (HA), Me hippuric acid(2-MHA, 3-MHA, 4-MHA) in urine. The target analytes in 0.5 mL urine sample were extracted with 0.5 mL Et acetate as extraction solvent in 3 min after the addition of 75 mg NaCL. After centrifugation, the four metabolites in sample were well separated on symmetry C18 (150 mm*4.6 mm, 5μm) column with mobile phase consisting of methanol: water: glacial acetic acid=30:70:0.1, column temperature at 35°C, the wavelength was set at 235 nm. Under the optimal conditions, the calibration curves of the four metabolites were linear in the range of 20mg/L∼500mg/L and the correlation coefficients were not less than 0.9997. The detection limits (S/N=3) were in the range of 21μg/L-200μg/L. The spiked recoveries were in the range of 97%∼104% with relative standard deviations (RSD) less than 5%. The method was sensitive, accurate and it could meet the needs of biol. monitoring on the metabolites of harmful substances in urine. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).COA of Formula: C10H11NO3
The Article related to urinary metabolite methyl hippuric acid hplc, Food and Feed Chemistry: Analysis and other aspects.COA of Formula: C10H11NO3
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