On December 29, 2017, Lunn, Daniel B.; Yun, Young J.; Jorgenson, James W. published an article.Product Details of 27115-50-0 The title of the article was Retention and effective diffusion of model metabolites on porous graphitic carbon. And the article contained the following:
The study of metabolites in biol. samples is of high interest for a wide range of biol. and pharmaceutical applications. Reversed phase liquid chromatog. is a common technique used for the separation of metabolites, but it provides little retention for polar metabolites. An alternative to C18 bonded phases, porous graphitic carbon has the ability to provide significant retention for both non-polar and polar analytes. The goal of this work is to study the retention and effective diffusion properties of porous graphitic carbon, to see if it is suitable for the wide injection bands and long run times associated with long, packed capillary-scale separations The retention of a set of standard metabolites was studied for both stationary phases over a wide range of mobile phase conditions. Porous graphitic carbon benefits from significantly increased retention (often >100 fold) under initial gradient conditions for these metabolites, suggesting much improved ability to focus a wide injection band at the column inlet. The effective diffusion properties of these columns were studied using peak-parking experiments with the standard metabolites under a wide range of retention conditions. Under the high retention conditions, which can be associated with retention after injection loading for gradient separations, Deff/Dm ∼ 0.1 for both the C18-bonded and porous graphitic carbon columns. As C18 bonded particles are widely, and successfully used for long gradient separations without issue of increasing peak width from longitudinal diffusion, this suggests that porous graphitic carbon should be amenable for long runtime gradient separations as well. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Product Details of 27115-50-0
The Article related to metabolite retention diffusion porous graphitic carbon chromatog, effective diffusion, metabolites, porous graphitic carbon, sample pre-concentration, Biochemical Methods: Chromatographic and other aspects.Product Details of 27115-50-0
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