On March 20, 2013, Liu, Jie; Mei, Yong; Wang, Ya-nan; Gu, Ying-ying; Wang, Qian; Jiang, Jin-feng published an article.Category: amides-buliding-blocks The title of the article was Standardized study on determination of methylhippuric acids in urine by HPLC. And the article contained the following:
The aim of this paper is to get a better separation of methylhippuric acid isomers and establish a standardized method for the determination of methylhippuric acids in urine by HPLC. C18 column was used to determine the methylhippuric acid. The mobile phase was methanol-0.1% acetic acid (33:67). The detector was performed at λ=230 nm, at 0.7 mVmin. Calibration curves of three methylhippuric acids were linear in the range of 25μg/mL∼1000μg/mL, with a correlation coefficient more than 0.999. The average recovery was in the range of 91.36%∼101.36% and the average RSD was in the range of 2.0%∼9.6%. The min. detection concentrations of 2-MHA, 3-MHA, 4-MHA were resp. 0.47μg/mL, 0.46μg/mL, 0.41μg/mL. The separation of 4-methylhippuric acid and 3-methylhippuric acid was up to 1.44. The method is able to make 3-methylhippuric acid and 4-methylhippuric acid baseline separated, and the methodol. indicators are in line with the relevant requirements of the method for determination of chems. in the occupational health standard-setting Guide Part V. So it can be used to determine methylhippuric acids in urine of xylene exposure workers. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Category: amides-buliding-blocks
The Article related to hplc methylhippuric acid determination, Biochemical Methods: Chromatographic and other aspects.Category: amides-buliding-blocks
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics