On July 15, 2013, Gagne, Sebastien published an article.Reference of 2-(4-Methylbenzamido)acetic acid The title of the article was Achieving greater selectivity for the analysis of o-, m-, p-methylhippuric acids in workers’ urine by ultra performance liquid chromatography coupled with tandem mass spectrometry. And the article contained the following:
A selective method analyzing sep. o-, m- and p-methylhippuric acid isomers in workers’ urine samples has been developed using ultra performance liquid chromatog. coupled with tandem mass spectrometry. Chromatog. separation has been optimized to resolve the three isomers at baseline. Combined with this optimal separation, the mass spectrometer allowed rapid switching from MRM scan to full scan and product ion scan within the chromatog. peak. This feature allowed the retention of analyte chem. structure information for the three methylhippuric acid isomers in parallel with the simultaneous acquisition of quant. data. Such an approach is unequaled for the reliability of the data generated and it can be applied to each isomer sep. The method was adjusted to a dynamic range between 0.2 mM and 8.12 mM for o-methylhippuric acid and p-methylhippuric acid, and between 0.41 mM and 16.23 mM for m-methylhippuric acid to cover the biol. exposure index. A negligible matrix effect was observed with the conditions used. Also, intra-day and inter-day precisions were both <6% for all the concentration levels tested and the accuracy was evaluated at 97%. The inclusion of simultaneous full scan acquisitions did not prevent the robustness of the quant. data. The method applied to the determination of inter-laboratory proficient test samples led to results in the tolerated range. Moreover, urine samples from workers were robustly quantified and the MHA levels were all below the biol. exposure index reference value. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid
The Article related to xylene exposure methylhippurate urine analysis worker, Toxicology: Methods (Including Analysis) and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid
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