Abdul, Shiraazkhan; Dekkers, Dick H. W.; Ariens, Robert A. S.; Leebeek, Frank W. G.; Rijken, Dingeman C.; Uitte de Willige, Shirley published an article in 2020, the title of the article was On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form.COA of Formula: C2H4ClNO And the article contains the following content:
The C-terminus of α2AP is crucial for the initial interaction with plasmin(ogen) and the rapid inhibitory mechanism. Approx. 35% of circulating α2AP has lost its C-terminus (non-plasminogen binding α2AP/NPB-α2AP) and thereby its rapid inhibitory capacity. The C-terminal cleavage site of α2AP is still unknown. A com. available monoclonal antibody against α2AP (TC 3AP) detects intact but not NPB-α2AP, suggesting that the cleavage site is located N-terminally from the epitope of TC 3AP. For epitope mapping of TC 3AP, com. available plasma purified α2AP was enzymically digested with Asp-N, Glu-C, or Lys-N. The resulting peptides were immunoprecipitated using TC 3AP-loaded Dynabeads Protein G. Bound peptides were eluted and analyzed by liquid chromatog.-tandem mass spectometry (LC-MS/MS). To localize the C-terminal cleavage site precisely, α2AP (intact and NPB) was purified from plasma and analyzed by LC-MS/MS after enzymic digestion with Arg-C. LC-MS/MS data from plasma purified α2AP showed that NPB-α2AP results from cleavage at Gln421-Asp422 as preferred site, but also after Leu417, Glu419, Gln420, or Asp422. The C-terminal cleavage site of human α2AP is located N-terminally from the TC 3AP epitope. Because C-terminal cleavage of α2AP can occur after multiple residues, different proteases may be responsible for the generation of NPB-α2AP. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).COA of Formula: C2H4ClNO
The Article related to alpha 2 antiplasmin amino acid proteolysis protein localization, alpha-2-antiplasmin, epitope mapping, mass spectrometry, proteolysis, western blot, Placeholder for records without volume info and other aspects.COA of Formula: C2H4ClNO
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