On March 31, 2019, Hu, Cong; Yang, Zhen; Yan, Fuhua; Sun, Biancheng published an article.Formula: C10H11NO3 The title of the article was Extraction of the toluene exposure biomarkers hippuric acid and methylhippuric acid using a magnetic molecularly imprinted polymer, and their quantitation by LC-MS/MS. And the article contained the following:
A magnetic polymer was molecularly imprinted with hippuric acid (HA) to obtain a nanomaterial with an architecture of type Fe3O4@SiO2@MIP. It was used as a sorbent for magnetic solid phase extraction of HA and methylhippuric acids (2-MHA, 3-MHA, 4-MHA) from urine samples. The resp. imprinting factor are 4.6, 2.7, 2.0 and 1.8, resp., and the selectivity coefficients are 1.7, 2.3 and 2.6. The effects of adsorbent amount, extraction time, pH value, ionic strength, desorption solvent and desorption time on the extraction of HA and MHA were investigated. Following elution with 1 mM NaOH solution, the 4 HAs were quantified by ultra-performance liquid chromatog. with mass spectrometric detection. Under the optimal exptl. conditions, the limits of detection (at S/N = 3) range between 89 ng·L-1 (for HA) and 170 ng·L-1 (for 4-MHA), the limits of quantification (at S/N = 10) range between 300 ng·L-1 (for HA) and 570 ng·L-1 (for 4-MHA), the linear range extends from 500 ng·L-1 to 10 mg·L-1, the relative standard deviations of intra-day range between 6.4 and 9.6% (for n = 6 at 10μg·L-1) and inter-day range between 9.2 and 11.5% (for n = 6 at 10μg·L-1). The method was successfully applied to the anal. of HA and MHA in (spiked) urine, and good recoveries were obtained. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Formula: C10H11NO3
The Article related to lc ms toluene biomarker hippuric methylhippuric acid polymer china, fourier transform infrared spectrometry, magnetic solid phase extraction, transmission electron microscopy, ultrasonic liquid desorption, urine analysis, vortex-assisted extraction, x-ray photoelectron spectroscopy and other aspects.Formula: C10H11NO3
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