In 2018,Yu, Chenfei; Tang, Juan; Loredo, Axel; Chen, Yuda; Jung, Sung Yun; Jain, Antrix; Gordon, Aviva; Xiao, Han published 《Proximity-Induced Site-Specific Antibody Conjugation》.Bioconjugate Chemistry published the findings.Safety of tert-Butyl N,N’-diisopropylcarbamimidate The information in the text is summarized as follows:
Site-specific antibody conjugates with a well-defined structure and superb therapeutic index are of great interest for basic research, disease diagnostics, and therapy. Here, we develop a novel proximity-induced antibody conjugation strategy enabling site-specific covalent bond formation between functional moieties and native antibodies without antibody engineering or addnl. UV/chem. treatment. A high conjugation efficiency and specificity was achieved with IgGs from different species and subclasses. The utility of this approach was demonstrated by site-specific conjugation of the small-mol. fluorophore to a native antibody and in vitro characterization of its activities. The results came from multiple reactions, including the reaction of tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8Safety of tert-Butyl N,N’-diisopropylcarbamimidate)
tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8) belongs to anime. The reaction of alkyl halides, R―X, where X is a halogen, or analogous reagents with ammonia (or amines) is useful with certain compounds. Not all alkyl halides are effective reagents; the reaction is sluggish with secondary alkyl groups and fails with tertiary ones. Its usefulness is largely confined to primary alkyl halides (those having two hydrogen atoms on the reacting site).Safety of tert-Butyl N,N’-diisopropylcarbamimidate
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics