The author of 《Exploring Pairwise Chemical Crosslinking To Study Peptide-Receptor Interactions》 were Seidel, Lisa; Zarzycka, Barbara; Katritch, Vsevolod; Coin, Irene. And the article was published in ChemBioChem in 2019. Category: amides-buliding-blocks The author mentioned the following in the article:
Pairwise crosslinking is a powerful technique to characterize interactions between G protein coupled receptors and their ligands in the live cell. In this work, the “”thiol trapping”” method, which exploits the proximity-enhanced reaction between haloacetamides and cysteine, is examined to identify intermol. pairs of vicinal positions. By incorporating cysteine into the corticotropin-releasing factor receptor and either a-chloro- or a-bromoacetamide groups into its ligands, it is shown that thiol trapping provides highly reproducible signals and a low background, and represents a valid alternative to classical “”disulfide trapping””. The method is advantageous if reducing agents are required during sample anal. Moreover, it can provide partially distinct spatial constraints, thus giving access to a wider dataset for mol. modeling. Finally, by applying recombinant mini-Gs, GTPS, and Gas-depleted HEK293 cells to modulate Gs coupling, it is shown that yields of crosslinking increase in the presence of elevated levels of Gs. In the experiment, the researchers used many compounds, for example, 2-Bromoacetamide(cas: 683-57-8Category: amides-buliding-blocks)
2-Bromoacetamide(cas: 683-57-8) can be used in preparation of (2-carbamoylmethoxy-5-chloro-benzyl)-carbamic acid tert-butyl ester. It was aslo used as precursor to dehydropeptidase I inactivator.Category: amides-buliding-blocks
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics