《An in-line capillary electrophoresis assay for the high-throughput screening of histone deacetylase inhibitors》 was written by Li, Yue; Fang, Hao; Hou, Zhun; Sang, Lihong; Yang, Xinying. Recommanded Product: H-Lys(Boc)-OH And the article was included in Journal of Chromatography A on April 26 ,2019. The article conveys some information:
Histone deacetylases (HDACs) are important enzymes that cause chromatin structure contraction and transcription repression, which can downregulate some cancer-suppression genes and lead to the occurrence of cancer. HDAC-specific inhibition is an effective approach to cancer therapy. Hence, a method with which to investigate HDAC activity is needed. We developed an in-line capillary electrophoresis method based on electrophoretically mediated microanal. The optimized conditions were thoroughly validated, and the method was applied to determine the enzyme’s kinetic parameters and the inhibition characteristics of three potent probe inhibitors. The obtained values were comparable to the literature data. Hence, the presented method, with its advantages of miniaturization and full automation, could be used for kinetic and inhibition studies of HDACs, which are targets for drug discovery, in the early stages of new drug development. In the experiment, the researchers used many compounds, for example, H-Lys(Boc)-OH(cas: 2418-95-3Recommanded Product: H-Lys(Boc)-OH)
H-Lys(Boc)-OH(cas: 2418-95-3) belongs to amino acids. Amino acids are not generally considered to be electrochemically active because products of the oxidation accumulate on the electrode surface and prevent it from participating in any further electrochemical processes.Recommanded Product: H-Lys(Boc)-OH
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