《Chemical synthesis of transactivation domain (TAD) of tumor suppressor protein p53 by native chemical ligation of three peptide segments》 was written by Baral, Abhishek; Asokan, Aromal; Bauer, Valentin; Kieffer, Bruno; Torbeev, Vladimir. Safety of 2-BromoacetamideThis research focused ontransactivation domain tumor suppressor protein p53 total synthesis NMR; solid phase peptide synthesis thioesterification native chem ligation alkylation; post translational modification protein folding binding NCBD conformation CD; NCBD nuclear coactivator binding domain CREB binding protein. The article conveys some information:
Chem. composition of tumor suppressor protein p53 is altered via multiple post-translational modifications which modulate its cellular lifetime and interactions with other biomols. Here we report total chem. synthesis of a 61-residue form of transactivation domain (TAD) of p53 based on native chem. ligation of three peptide segments. The experiments to characterize its binding to nuclear co-activator binding domain (NCBD) of CREB-binding protein confirmed native-like induced folding upon binding to NCBD. Thus, the synthetic approach described herein can be useful for the preparation of various post-translationally modified analogs of TAD-p53 for further functional biochem. and biophys. studies. The results came from multiple reactions, including the reaction of 2-Bromoacetamide(cas: 683-57-8Safety of 2-Bromoacetamide)
2-Bromoacetamide(cas: 683-57-8) can be used in preparation of (2-carbamoylmethoxy-5-chloro-benzyl)-carbamic acid tert-butyl ester. It was aslo used as precursor to dehydropeptidase I inactivator.Safety of 2-Bromoacetamide
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