Yin, Yaguang; Kong, Xiuqi; Li, Min; Wang, Jingchao; Dai, Xiaoyu; Zhang, Yunyan; Lin, Weiying published the artcile< Development of an esterase fluorescent probe based on naphthalimide-benzothiazole conjugation and its applications for qualitative detection of esterase in orlistat-treated biosamples>, Application of C29H53NO5, the main research area is ESIPT; Esterase fluorescent probe; Fast response; Orlistat-treated cell imaging; Zebrafish imaging.
Esterase is a large hydrolysis family, and widely distributed in many kinds of cells. It is responsible for multiple physiol. and pathol. functions including metabolism, gene expression. While abnormality of esterase is associated with many pathol. activities in obesity, Wolman′s disease, and cancer. Thereby, it is essential to design an effective tool for esterase in situ detection in biol. systems. Herein, a novel fluorescent probe Y-1 for monitoring esterase in living cells was rationally designed. Probe Y-1 was synthesized by the conjugation between an acetylation of 4-hydroxy naphthalimide and benzothiazole group. Benzothiazole moiety is a typical Excited-state intramol. proton transfer (ESIPT) controller. Acetate group was selected as the responsive site and ESIPT initiator. As the acetate group could block the ESIPT effect, the probe emits no fluorescence under the excitation of 455 nm. When binding with esterase, Y-1 shows distinct fluorescence with the peak at 560 nm with short time when ESIPT is on. Y-1 displays high sensitivity (LOD is 0.216 x 10-3 U/mL), fast response (within 5 min), high selectivity and photostability towards esterase. Furthermore, the %RSD (relative standard deviation) of within-day and day-to-day precision was no more than 13.0% and the accuracy ranged from -6.5 to -12.3%. Kinetics performance of Y-1 indicates that esterase has high affinity and hydrolysis to Y-1. For biol. applications, our probe is a time-dependent visualizing esterase in living HepG2 and CoLo205 cells within 15 min. After the treatment of orlistat (1 and 5 μM) for inhibiting the activity of esterase, the bright fluorescence has also been detected using our probe. Furthermore, it has been successful in monitoring the esterase in zebrafish, the data were consistent with cellular phenomena. Therefore, all these findings indicate that the robust probe Y-1 is a useful qual. tool for detecting esterase in biol. systems.
Analytica Chimica Acta published new progress about 96829-58-2. 96829-58-2 belongs to class amides-buliding-blocks, and the molecular formula is C29H53NO5, Application of C29H53NO5.
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics