In 2019,Journal of the American Chemical Society included an article by Du, Kang; Thorarinsdottir, Agnes E.; Harris, T. David. Synthetic Route of C2H4BrNO. The article was titled 《Selective Binding and Quantitation of Calcium with a Cobalt-Based Magnetic Resonance Probe》. The information in the text is summarized as follows:
The authors report a cobalt-based paramagnetic chem. exchange saturation transfer (PARACEST) magnetic resonance (MR) probe that is able to selectively bind and quantitate the concentration of Ca2+ under physiol. conditions. The parent LCoII complex features an uncoordinated crown ether moiety in close proximity to the CoII center. Addition of Na+ or Ca2+ leads to binding of these metal ions within the crown ether. Single-crystal x-ray diffraction and solid-state magnetic measurements reveal the presence of a metal-specific coordination environment and magnetic anisotropy at Co, with the axial zero-field splitting parameter of the Na+ and Ca2+ complex differing by over 90%. Owing to these differences, solution-based measurements under physiol. conditions indicate reversible binding of Na+ and Ca2+ to give well-separated CEST peaks at 69 and 80 ppm, resp. Dissociation constants for different cation-bound complexes of LCo, as determined by 1H NMR spectroscopy, demonstrate high selectivity toward Ca2+. This finding, in conjunction with the large excess of Na+ in physiol. environments, minimizes interference from related cations, such as Mg2+ and K+. Finally, variable-[Ca2+] CEST spectra establish the ratio between the CEST peak intensities for the Ca2+- and Na+-bound probes (CEST80 ppm/CEST69 ppm) as a measure of [Ca2+], providing the first example of a ratiometric quantitation of Ca2+ concentration using PARACEST. Taken together, these results demonstrate the ability of transition metal PARACEST probes to afford a concentration-independent measure of [Ca2+], and provide a new approach for designing MR probes for cation sensing. The experimental part of the paper was very detailed, including the reaction process of 2-Bromoacetamide(cas: 683-57-8Synthetic Route of C2H4BrNO)
2-Bromoacetamide(cas: 683-57-8) can be used in preparation of (2-carbamoylmethoxy-5-chloro-benzyl)-carbamic acid tert-butyl ester. It was aslo used as precursor to dehydropeptidase I inactivator.Synthetic Route of C2H4BrNO
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics