In 2017,Lin, Tse-Hsueh; Lin, Cin-Hao; Liu, Ying-Jie; Huang, Chun Yi; Lin, Yen-Cheng; Wang, Sheng-Kai published 《Controlling Ligand Spacing on Surface: Polyproline-Based Fluorous Microarray as a Tool in Spatial Specificity Analysis and Inhibitor Development for Carbohydrate-Protein Interactions》.ACS Applied Materials & Interfaces published the findings.Safety of tert-Butyl N,N’-diisopropylcarbamimidate The information in the text is summarized as follows:
Multivalent carbohydrate-protein interactions are essential for many biol. processes. Convenient characterization for multivalent binding property of proteins will aid the development of mols. to manipulate these processes. The authors exploited the polyproline helix II (PPII) structure as mol. scaffolds to adjust the distances between glycan ligand attachment sites at 9, 18, and 27 Å on a peptide scaffold. Optimized fluorous groups were also introduced to the peptide scaffold for immobilization to the microarray surface through fluorous interaction to control the orientation of the helical scaffolds. Using lectin LecA and antibody 2G12 as model proteins, the binding preference to the 27 Å glycopeptide scaffold, matched the distance of 26 Å between its two galactose binding sites on LecA and 31 Å spacing between oligomannose binding sites on 2G12, resp. The authors further demonstrate this microarray system can aid the development of inhibitors by transforming the selected surface-bound scaffold into multivalent ligands in solution This strategy can be extended to analyze proteins that lacking structural information to speed up the design of potent and selective multivalent ligands.tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8Safety of tert-Butyl N,N’-diisopropylcarbamimidate) was used in this study.
tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8) belongs to anime. Nitrous acid converts secondary amines (aliphatic or aromatic) to N-nitroso compounds (nitrosamines): R2NH + HNO2 → R2N―NO. Some nitrosamines are potent cancer-inducing substances, and their possible formation is a serious consideration when nitrites, which are salts of nitrous acid, are present in foods or pharmaceutical preparations. Tertiary amines give rise to nitrosamines more slowly; an alkyl group is eliminated as an aldehyde or ketone, along with nitrous oxide, N2O.Safety of tert-Butyl N,N’-diisopropylcarbamimidate
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics