The author of 《Site-Specific Encoding of Photoactivity in Antibodies Enables Light-Mediated Antibody-Antigen Binding on Live Cells》 were Bridge, Thomas; Shaikh, Saher A.; Thomas, Paul; Botta, Joaquin; McCormick, Peter J.; Sachdeva, Amit. And the article was published in Angewandte Chemie, International Edition in 2019. Safety of H-Lys(Boc)-OH The author mentioned the following in the article:
Antibodies found applications in several fields, including, medicine, diagnostics, and nanotechnol., yet methods to modulate antibody-antigen binding using an external agent remain limited. Here, the authors have developed photoactive antibody fragments by genetic site-specific replacement of single tyrosine residues with photocaged tyrosine, in an antibody fragment, 7D12. A simple and robust assay is adopted to evaluate the light-mediated binding of 7D12 mutants to its target, epidermal growth factor receptor (EGFR), on the surface of cancer cells. Presence of photocaged tyrosine reduces 7D12-EGFR binding affinity by over 20-fold in two out of three 7D12 mutants studied, and binding is restored upon exposure to 365 nm light. Mol. dynamics simulations explain the difference in effect of photocaging on 7D12-EGFR interaction among the mutants. Finally, the authors demonstrate the application of photoactive antibodies in delivering fluorophores to EGFR-pos. live cancer cells in a light-dependent manner. In the experimental materials used by the author, we found H-Lys(Boc)-OH(cas: 2418-95-3Safety of H-Lys(Boc)-OH)
H-Lys(Boc)-OH(cas: 2418-95-3) belongs to amino acids. In addition to subunits of proteins, amino acids have many other functions as well, including osmoregulation (proline), neurotransmitters (gamma-aminobutyric acid), metabolic intermediates (ornithine and citrulline), and inhibitors (dehydroproline).Safety of H-Lys(Boc)-OH
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics