《Initiation of protein synthesis with non-canonical amino acids in vivo》 was published in Angewandte Chemie, International Edition in 2020. These research results belong to Tharp, Jeffery M.; Ad, Omer; Amikura, Kazuaki; Ward, Fred R.; Garcia, Emma M.; Cate, Jamie H. D.; Schepartz, Alanna; Soell, Dieter. Quality Control of H-Lys(Boc)-OH The article mentions the following:
By transplanting identity elements into E. coli tRNAfMet, we have engineered an orthogonal initiator tRNA (itRNATy2) that is a substrate for Methanocaldococcus jannaschii TyrRS. We demonstrate that itRNATy2 can initiate translation in vivo with aromatic non-canonical amino acids (ncAAs) bearing diverse sidechains. Although the initial system suffered from low yields, deleting redundant copies of tRNAfMet from the genome afforded an E. coli strain in which the efficiency of non-canonical initiation equals elongation. With this improved system we produced a protein containing two distinct ncAAs at the first and second positions, an initial step towards producing completely unnatural polypeptides in vivo. This work provides a valuable tool to synthetic biol. and demonstrates remarkable versatility of the E. coli translational machinery for initiation with ncAAs in vivo. The experimental process involved the reaction of H-Lys(Boc)-OH(cas: 2418-95-3Quality Control of H-Lys(Boc)-OH)
H-Lys(Boc)-OH(cas: 2418-95-3) belongs to amino acids. Amino acids are not generally considered to be electrochemically active because products of the oxidation accumulate on the electrode surface and prevent it from participating in any further electrochemical processes.Quality Control of H-Lys(Boc)-OH
Referemce:
Amide – Wikipedia,
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