《Nicotinamide and structurally related compounds show halting activity against zoospores of the phytopathogenic fungus Aphanomyces cochlioides》 was written by Shimai, Takashi; Islam, Tofazzal Md.; Fukushi, Yukiharu; Hashidoko, Yasuyuki; Yokosawa, Ryozo; Tahara, Satoshi. HPLC of Formula: 89281-13-0 And the article was included in Zeitschrift fuer Naturforschung, C: Journal of Biosciences on April 30 ,2002. The article conveys some information:
In a survey of plant secondary metabolites regulating the behavior of phytopathogenic Aphanomyces cochlioides zoospores, we found that leaf extracts of Amaranthus gangeticus and cotyledon extracts of pea (Pisum sativum) remarkably halted the motility of zoospores. Bioassay-directed fractionation of A. gangeticus and pea constituents revealed that the halting activity was dependent on a single chem. factor (halting factor). The active principle was identified as nicotinamide (1) by comparing its biol. activity and spectroscopic properties with those of the authentic compound Nicotinamide (1) showed potent halting activity toward the zoospores of A. cochlioides and A. euteiches, but it exhibited very less activity against other Oomycetes, Pythium aphanidermatum and Phytophthora infestans zoospores. Interestingly, the zoospores halted by nicotinamide (1) encysted within 10-15 min and then the resulting cystospores regenerated zoospores instead of germination. Nicotinamide (1) and related compounds were subjected to the halting activity bioassay to elucidate the structure-activity relationships. These bioassays revealed that part structures of (A) the aromatic ring containing at least one nitrogen atom, (B) carbonyl-like group adjacent to the aromatic ring and (C) hydrogen atoms on the amide group are responsible for the strong activity. So far, this is the first report of halting activity of nicotinamide (1) against fungal zoospores. In the experiment, the researchers used 2,6-Dichloroisonicotinamide(cas: 89281-13-0HPLC of Formula: 89281-13-0)
2,6-Dichloroisonicotinamide(cas: 89281-13-0) belongs to anime. Reduction of nitro compounds, RNO2, by hydrogen or other reducing agents produces primary amines cleanly (i.e., without a mixture of products), but the method is mostly used for aromatic amines because of the limited availability of aliphatic nitro compounds. Reduction of nitriles and oximes (R2C=NOH) also yields primary amines.HPLC of Formula: 89281-13-0
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics