Rawson, Frankie J.;Downard, Alison J.;Baronian, Keith H. published 《Electrochemical detection of intracellular and cell membrane redox systems in Saccharomyces cerevisiae》 in 2014. The article was appeared in 《Scientific Reports》. They have made some progress in their research.Application of 329-89-5 The article mentions the following:
Redox mediators can interact with eukaryote cells at a number of different cell locations. While cell membrane redox centers are easily accessible, the redox centers of catabolism are situated within the cytoplasm and mitochondria and can be difficult to access. We have systematically investigated the interaction of thirteen commonly used lipophilic and hydrophilic mediators with the yeast Saccharomyces cerevisiae. A double mediator system is used in which ferricyanide is the final electron acceptor (the reporter mediator). After incubation of cells with mediators, steady state voltammetry of the ferri/ferrocyanide redox couple allows quantitation of the amount of mediator reduced by the cells. The plateau current at 425 mV vs Ag/AgCl gives the anal. signal. The results show that five of the mediators interact with at least three different trans Plasma Membrane Electron Transport systems (tPMETs), and that four mediators cross the plasma membrane to interact with cytoplasmic and mitochondrial redox mols. Four of the mediators inhibit electron transfer from S. cerevisiae. Catabolic inhibitors were used to locate the cellular source of electrons for three of the mediators. To complete the study, the researchers used 6-Aminonicotinamide (cas: 329-89-5) .
6-Aminonicotinamide (cas:329-89-5)Application of 329-89-5 is an inhibitor of the NADP+-dependent enzyme, PGD (6-phosphogluconate dehydrogenase). Studies have also shown that 6-aminonicotinamide induces apoptosis in tumor cells and causes glial cell degeneration.
Reference:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics